In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
30489693
DOI
10.1002/cpnc.71
Knihovny.cz E-zdroje
- Klíčová slova
- DNA, eukaryotic cell, in-cell NMR spectroscopy, mammalian cell,
- MeSH
- buněčné jádro metabolismus MeSH
- cytosol metabolismus MeSH
- elektroporace MeSH
- HEK293 buňky MeSH
- HeLa buňky MeSH
- konfokální mikroskopie MeSH
- lidé MeSH
- nukleární magnetická rezonance biomolekulární metody MeSH
- nukleové kyseliny metabolismus MeSH
- průtoková cytometrie MeSH
- transfekce MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- nukleové kyseliny MeSH
In-cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near-to-atomic resolution. In this article, detailed instructions are described for setting up an in-cell NMR experiment for monitoring structures of DNA oligonucleotides introduced into nuclei of living human cells via tailored electroporation. Detailed step-by-step protocols for both the preparation of an in-cell NMR sample as well as protocols for conducting essential control experiments including flow cytometry and confocal microscopy are described. The strengths and limitations of in-cell NMR experiments are discussed. © 2018 by John Wiley & Sons, Inc.
Central European Institute of Technology Masaryk University Brno Czech Republic
Institute of Biophysics v v i Czech Academy of Sciences Brno Czech Republic
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