In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30489693
DOI
10.1002/cpnc.71
Knihovny.cz E-resources
- Keywords
- DNA, eukaryotic cell, in-cell NMR spectroscopy, mammalian cell,
- MeSH
- Cell Nucleus metabolism MeSH
- Cytosol metabolism MeSH
- Electroporation MeSH
- HEK293 Cells MeSH
- HeLa Cells MeSH
- Microscopy, Confocal MeSH
- Humans MeSH
- Nuclear Magnetic Resonance, Biomolecular methods MeSH
- Nucleic Acids metabolism MeSH
- Flow Cytometry MeSH
- Transfection MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nucleic Acids MeSH
In-cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near-to-atomic resolution. In this article, detailed instructions are described for setting up an in-cell NMR experiment for monitoring structures of DNA oligonucleotides introduced into nuclei of living human cells via tailored electroporation. Detailed step-by-step protocols for both the preparation of an in-cell NMR sample as well as protocols for conducting essential control experiments including flow cytometry and confocal microscopy are described. The strengths and limitations of in-cell NMR experiments are discussed. © 2018 by John Wiley & Sons, Inc.
Central European Institute of Technology Masaryk University Brno Czech Republic
Institute of Biophysics v v i Czech Academy of Sciences Brno Czech Republic
References provided by Crossref.org
A sodium/potassium switch for G4-prone G/C-rich sequences
A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins
