Ultraviolet-B (UV-B) radiation is a key environmental signal which initiates diverse responses that affect the metabolism, development, and viability of plants. In keeping with our previous studies, we concentrated primarily on how UV-B radiation affects Norway spruce [Picea abies (L.) Karst.] somatic embryo maturation and how phenolics and polyamines (PAs) are linked to the defense response invoked by UV-B irradiation. We treated clusters of Norway spruce embryogenic culture (EC) with UV-B during the five stages of embryo maturation (early, cylindrical, precotyledonary, cotyledonary, and mature embryos). For the first time, we take an advantage of the unique environmental scanning electron microscope AQUASEM II to characterize somatic embryos in their native state. The severity of the irradiation effect on embryonal cell viability was shown to be dependent on the intensity of radiation as well as the stage of embryo development, and might be related to the formation of protoderm. The response of early embryos was characterized by an increase in malondialdehyde (MDA), a marked decrease in PA contents and a decline in phenolics. The reduced ability to activate the defense system seems to be responsible not only for the severe cell damage and decrease in viability but also for the inhibition of embryo development. The significant reduction in spermidine (Spd), which has been reported to be crucial for the somatic embryo development of several coniferous species, may be causally linked to the limited development of embryos. The pronounced decrease in cell wall-bound ferulic acid might correspond to failure of somatic embryos to reach more advanced stages of development. Embryos at later stages of development showed stress defense responses that were more efficient against UV-B exposure.

Institute of Experimental Botany of the Czech Academy of Sciences Prague Czechia

Institute of Scientific Instruments of the Czech Academy of Sciences Brno Czechia

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Agati G., Tattini M. (2010). Multiple functional roles of flavonoids in photoprotection. PubMed DOI

Binarová P., Cvikrová M., Havlický T., Eder J., Plevková J. (1994). Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability. DOI

Booij-James I. S., Dube S. K., Jansen M. A. K., Edelman M., Mattoo A. K. (2000). Ultraviolet-B radiation impacts light-mediated turnover of the photosystem II reaction center heterodimer in PubMed DOI PMC

Bouchereau A., Aziz A., Larher F., Martin-Tanguy J. (1999). Polyamines and environmental challenges: recent development. DOI

Cvikrová M., Gemperlová L., Eder J., Zažímalová E. (2008). Excretion of polyamines in alfalfa and tobacco suspension-cultured cells and its possible role in maintenance of intracellular polyamine contents. PubMed DOI

Cvikrová M., Gemperlová L., Martincová O., Vačková R. (2013). Effect of drought and combined drought and heat stress on polyamine metabolism in proline-over-producing tobacco plants. PubMed DOI

Cvikrová M., Malá J., Hrubcová M., Martincová O., Cvrčková H., Lipavská H. (2010). Defence responses induced in embryogenic cultures of Norway spruce by two fractions of DOI

Cvikrová M., Meravý L., Macháčková I., Eder J. (1991). Phenylalanine ammonia-lyase, phenolic acids and ethylene in alfalfa ( PubMed DOI

Cvikrová M., Vondrákova Z., Eliášová K., Pešek B., Trávníčková A., Vágner M. (2016). The impact of UV-B irradiation applied at different phases of somatic embryo development in Norway spruce on polyamine metabolism. DOI

Eliášová K., Vondráková Z., Malbeck J., Trávníčková A., Pešek B., Vágner M., et al. (2017). Histological and biochemical response of Norway spruce somatic embryos to UV-B irradiation. DOI

Filonova L. H., von Arnold S., Daniel G., Bozhkov P. V. (2002). Programmed cell death eliminates all but one embryo in a polyembryonic plant seed. PubMed DOI

Fry S. C. (1987). Intracellular feruloylation of pectic polysaccharides. PubMed DOI

Gemperlová L., Fischerová L., Cvikrová M., Malá J., Vondráková Z., Martincová O., et al. (2009). Polyamine profiles and biosynthesis in somatic embryo development and comparison of germinating somatic and zygotic embryos of Norway spruce. PubMed DOI

Gill S. S., Tuteja N. (2010). Polyamines and abiotic stress tolerance in plants. PubMed DOI PMC

Gupta P. K., Durzan D. J. (1986). Somatic polyembryogenesis from callus of mature sugar pine embryos.

Hideg É., Jansen M. A. K., Strid Å. (2013). UV-B exposure, ROS, and stress: inseparable companions or loosely linked associates? PubMed DOI

Hu X. H., Zhang Y., Shi Y., Zhang Z., Zou Z. R., Zhang H., et al. (2012). Effect of exogenous spermidine on polyamine content and metabolism in tomato exposed to salinity–alkalinity mixed stress. PubMed DOI

Hussain S. S., Ali M., Ahmad M., Siddique K. H. M. (2011). Polyamines: natural and engineered abiotic and biotic stress tolerance in plants. PubMed DOI

Jansen M. A. K., Babu T. S., Heller D., Gaba V., Mattoo A. K., Edelman M. (1996). Ultraviolet-B effects on

Jansen M. A. K., Gaba V., Greenberg B. M. (1998). Higher plants and UV-B radiation: balancing damage, repair and acclimation.

Jansen M. A. K., Hectors K., O’Brien N. M., Guisez Y., Potters G. (2008). Plant stress and human health: do human consumers benefit from UV-B acclimated crops? DOI

Javelle M., Vernoud V., Rogowsky P. M., Ingram G. C. (2011). Epidermis: the formation and functions of a fundamental plant tissue. PubMed DOI

Kusano T., Berberich T., Tateda C., Takahashi Y. (2008). Polyamines: essential factors for growth and survival. PubMed DOI

Kuthanová A., Gemperlová L., Zelenková S., Eder J., Macháčková I., Opatrný Z., et al. (2004). Cytological changes and alterations in polyamine contents induced by cadmium in tobacco BY-2 cells. PubMed DOI

Laakso K., Sullivan J. H., Huttunen S. (2000). The effects of UV-B radiation on epidermal anatomy in loblolly pine ( DOI

Lozovaya V., Gorshkova T., Yablokova E., Zabotina O., Ageeva M., Rumyantseva N., et al. (1996). Callus cell wall phenolics and plant regeneration ability. DOI

Lütz C., Navakoudis E., Seidlitz H. K., Kotzabasis K. (2005). Simulated solar irradiation with enhanced UV-B adjust plastid- and thylakoid-associated polyamine changes for UV-B protection. PubMed DOI

Martin K. P., Madassery J. (2005). Direct and indirect somatic embryogenesis on cotyledon explants of DOI

Martínez-Abaigar J., Monforte L., Del-Castillo-Alonso M. Á., Fabón G., Tomás-Las-Heras R., Núñez-Olivera E. (2015). Ultraviolet-absorbing compounds from the cell walls of an aquatic liverwort are more efficiently extracted by alkaline than by enzymatic digestion. DOI

Minocha R., Minocha S. C., Long S. (2004). Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce ( DOI

Minocha R., Smith D. R., Reeves C., Steele K. D., Minocha S. C. (1999). Polyamine levels during the development of zygotic and somatic embryos of DOI

Moschou P. N., Paschalidis K. A., Delis I. D., Andriopoulou A. H., Lagiotis G. D., Yakoumakis D. I., et al. (2008). Spermidine exodus and oxidation in the apoplast induced by abiotic stress is responsible for H PubMed DOI PMC

Moschou P. N., Wu J., Cona A., Tavladoraki P., Angelini R., Roubelakis-Angelakis K. A. (2012). The polyamines and their catabolic products are significant players in the turnover of nitrogenous molecules in plants. PubMed DOI

Neděla V., Hřib J., Vooková B. (2012). Imaging of early conifer embryogenic tissues with the environmental scanning electron microscope. DOI

Neděla V., Konvalina I., Oral M., Hudec J. (2015a). The simulation of energy distribution of electrons detected by segmental ionization detector in high pressure conditions of ESEM. DOI

Neděla V., Tihlaříková E., Hřib J. (2015b). The low-temperature method for study of coniferous tissues in the environmental scanning electron microscope. PubMed DOI

Pál M., Szalai G., Janda T. (2015). Speculation: polyamines are important in abiotic stress signaling. PubMed DOI

Papadakis A. K., Roubelakis-Angelakis K. A. (2005). Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide. PubMed DOI

Reifenrath K., Müller C. (2007). Species-specific and leaf-age dependent effects of ultraviolet radiation on two Brassicaceae. PubMed DOI

Reis E., Batista M. T., Canhoto J. M. (2008). Effect and analysis of phenolic compounds during somatic embryogenesis induction in PubMed DOI

Santanen A., Simola L. K. (1992). Changes in polyamine metabolism during somatic embryogenesis in Picea abies. DOI

Schenkmayerová A., Bučko M., Gemeiner P., Trel’ová D., Lacík I., Chorvát D., et al. (2014). Physical and bioengineering properties of polyvinyl alcohol lens-shaped particles versus spherical polyelectrolyte complex microcapsules as immobilisation matrices for a whole-cell Baeyer–Villiger monooxygenase. PubMed DOI

Schweiger J., Lang M., Lichtenthaler H. K. (1996). Differences in fluorescence excitation spectra of leaves between stressed and non-stressed plants. DOI

Schweikert K., Sutherland J. E. S., Hurd C. L., Burritt D. J. (2011). UV-B radiation induces changes in polyamine metabolism in the red seaweed DOI

Semerdjieva S. I., Sheffield E., Phoenix G. K., Gwynn-Jones D., Callaghan T. V., Johnson G. N. (2003). Contrasting strategies for UV-B screening in sub-arctic dwarf shrubs. PubMed DOI

Sfichi-Duke L., Ioannidis N. E., Kotzabasis K. (2008). Fast and reversible response of thylakoid-associated polyamines during and after UV-B stress: a comparative study of the wild type and a mutant lacking chlorophyll b of unicellular green alga PubMed DOI

Sheahan J. J. (1996). Sinapate esters provide greater UV-B attenuation than flavonoids in DOI

Shein I. V., Andreeva O. N., Polyakova G. G., Zrazhevskaya G. K. (2003). Effect of pine callus elicitation by the DOI

Shein I. V., Polyakova G. G., Zrazhevskaya G. K., Pashenova N. V., Vetrova V. P. (2001). Accumulation of phenolic compounds in conifer callus cultures in response to wood blue-stain fungi. DOI

Shi H., Chan Z. (2014). Improvement of plant abiotic stress tolerance through modulation of the polyamine pathway. PubMed DOI

Silveira V., Floh E. I. S., Handro W., Guerra M. P. (2004). Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of DOI

Smith J., Burrit D., Bannister P. (2001). Ultraviolet-B radiation leads to a reduction in free polyamines in DOI

Stasolla C., Kong L., Yeung E. C., Thorpe T. A. (2002). Maturation of somatic embryos in conifers: morphogenesis, physiology, biochemistry, and molecular biology. DOI

Svobodová H., Albrechtová J., Kumstýřová L., Lipavská H., Vágner M., Vondráková Z. (1999). Somatic embryogenesis in Norway spruce: anatomical study of embryo development and influence of polyethylene glycol on maturation process. DOI

Takahashi T., Kakehi J. I. (2010). Polyamines: ubiquitous polycations with unique roles in growth and stress responses. PubMed DOI PMC

Takshak S., Agrawal S. B. (2015). Defence strategies adopted by the medicinal plant PubMed DOI

Vondráková Z., Cvikrová M., Eliášová K., Martincová O., Vágner M. (2010). Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures. PubMed DOI

Vondráková Z., Eliášová K., Vágner M. (2014). The anti-actin drugs latrunculin and cytochalasin affect the maturation of spruce somatic embryos in different ways. PubMed DOI

Vondráková Z., Eliášová K., Vágner M., Martincová O., Cvikrová M. (2015). Exogenous putrescine affects endogenous polyamine levels and the development of DOI

Yiu J.-C., Liu C. W., Fang D. Y. T., Lai Y. S. (2009). Waterlogging tolerance of Welsh onion ( PubMed DOI

Zhu T., Moschou P. N., Alvarez J. M., Sohlberg J. J., von Arnold S. (2016). WUSCHEL-RELATED HOMEOBOX 2 is important for protoderm and suspensor development in the gymnosperm Norway spruce. PubMed DOI PMC

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