Poly(d,l-lactide)/polyethylene glycol micro/nanofiber mats as paclitaxel-eluting carriers: preparation and characterization of fibers, in vitro drug release, antiangiogenic activity and tumor recurrence prevention
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
30813105
DOI
10.1016/j.msec.2019.01.046
PII: S0928-4931(18)30868-3
Knihovny.cz E-resources
- Keywords
- Antiangiogenesis, CAM assay, Local tumor recurrence, Needleless electrospinning, PLA/PEG micro/nanofibers, Paclitaxel quantification,
- MeSH
- Cell Death drug effects MeSH
- X-Ray Diffraction MeSH
- Angiogenesis Inhibitors pharmacology MeSH
- Chickens MeSH
- Humans MeSH
- Neoplasm Recurrence, Local pathology prevention & control MeSH
- Mice, Nude MeSH
- Cell Line, Tumor MeSH
- Nanofibers chemistry ultrastructure MeSH
- Drug Carriers chemistry MeSH
- Paclitaxel pharmacology MeSH
- Polyesters chemistry MeSH
- Polyethylene Glycols chemistry MeSH
- Body Weight MeSH
- Temperature MeSH
- Tumor Burden drug effects MeSH
- Drug Liberation * MeSH
- Cell Survival drug effects MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Angiogenesis Inhibitors MeSH
- Drug Carriers MeSH
- Paclitaxel MeSH
- poly(lactide) MeSH Browser
- Polyesters MeSH
- Polyethylene Glycols MeSH
Poly(d,l-lactide)/polyethylene glycol (PLA/PEG) micro/nanofibers loaded with paclitaxel (PTX, 10 wt%) were prepared by needless electrospinning technology, which allows large scale production for real medicinal practice. The fiber structure and properties were investigated by several methods including scanning electron microscopy, nitrogen adsorption/desorption isotherm measurements, differential scanning calorimetry, and X-ray diffraction measurements to examine their morphology (fiber diameter distribution, specific surface area, and total pore volume), composition, drug-loading efficiency, and physical state. An HPLC-UV method was optimized and validated to quantify in vitro PTX release into PBS. The results showed that the addition of PEG into PLA fibers promoted the release of higher amounts of hydrophobic PTX over prolonged time periods compared to fibers without PEG. An in vitro cell assay demonstrated the biocompatibility of PLA/PEG fibrous materials and showed significant cytotoxicity of PTX-loaded PLA/PEG fibers against a human fibrosarcoma HT1080 cell line. The chick chorioallantoic membrane assay proved that PTX-loaded fibers exhibited antiangiogenic activity, with a pronounced effect in the case of the PEG-containing fibers. In vivo evaluation of PTX-loaded PLA/PEG fibers in a human fibrosarcoma recurrence model showed statistically significant inhibition in tumor incidence and growth after primary tumor resection compared to other treatment groups.
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