Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
BB/P01979X/1
Biotechnology and Biological Sciences Research Council - United Kingdom
PubMed
30860661
DOI
10.1002/cppb.20089
Knihovny.cz E-zdroje
- Klíčová slova
- chemically inducible gene expression, dexamethasone, glucocorticoid,
- MeSH
- aktivace transkripce * účinky léků MeSH
- Arabidopsis genetika MeSH
- dexamethason farmakologie MeSH
- DNA vazebné proteiny genetika MeSH
- genetické techniky MeSH
- geneticky modifikované rostliny genetika MeSH
- lac represory genetika MeSH
- promotorové oblasti (genetika) MeSH
- proteiny z Escherichia coli genetika MeSH
- receptory glukokortikoidů genetika MeSH
- Saccharomyces cerevisiae - proteiny genetika MeSH
- transgeny * MeSH
- transkripční faktory genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dexamethason MeSH
- DNA vazebné proteiny MeSH
- GAL4 protein, S cerevisiae MeSH Prohlížeč
- lac represory MeSH
- LacI protein, E coli MeSH Prohlížeč
- proteiny z Escherichia coli MeSH
- receptory glukokortikoidů MeSH
- Saccharomyces cerevisiae - proteiny MeSH
- transkripční faktory MeSH
Use of chemically inducible systems for transgene expression is a crucial requirement for modern plant biology research, as it allows (1) expression of transgenes that compromise plant viability or fertility when constitutively expressed and (2) spatiotemporal control of transgene expression levels. We describe the stringently regulated and highly responsive dexamethasone-inducible gene expression system pOp6/LhGR, which comprises the chimeric transcription activator LhGR and the corresponding pOp6 promoter. Upon induction, the LhGR activator binds to the pOp6 promoter and induces expression of the target gene of interest. We provide detailed protocols for inducing transgene expression at different developmental stages and in different plant species and discuss dexamethasone stability and use of its analogs. We also introduce new, versatile, GATEWAY-compatible binary vectors that are now available for the pOp6/LhGR system. © 2019 by John Wiley & Sons, Inc.
CEITEC MU Masaryk University Brno Czech Republic
Department of Plant Sciences University of Oxford Oxford United Kingdom
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