Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
BB/P01979X/1
Biotechnology and Biological Sciences Research Council - United Kingdom
PubMed
30860661
DOI
10.1002/cppb.20089
Knihovny.cz E-resources
- Keywords
- chemically inducible gene expression, dexamethasone, glucocorticoid,
- MeSH
- Transcriptional Activation * drug effects MeSH
- Arabidopsis genetics MeSH
- Dexamethasone pharmacology MeSH
- DNA-Binding Proteins genetics MeSH
- Genetic Techniques MeSH
- Plants, Genetically Modified genetics MeSH
- Lac Repressors genetics MeSH
- Promoter Regions, Genetic MeSH
- Escherichia coli Proteins genetics MeSH
- Receptors, Glucocorticoid genetics MeSH
- Saccharomyces cerevisiae Proteins genetics MeSH
- Transgenes * MeSH
- Transcription Factors genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dexamethasone MeSH
- DNA-Binding Proteins MeSH
- GAL4 protein, S cerevisiae MeSH Browser
- Lac Repressors MeSH
- LacI protein, E coli MeSH Browser
- Escherichia coli Proteins MeSH
- Receptors, Glucocorticoid MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Transcription Factors MeSH
Use of chemically inducible systems for transgene expression is a crucial requirement for modern plant biology research, as it allows (1) expression of transgenes that compromise plant viability or fertility when constitutively expressed and (2) spatiotemporal control of transgene expression levels. We describe the stringently regulated and highly responsive dexamethasone-inducible gene expression system pOp6/LhGR, which comprises the chimeric transcription activator LhGR and the corresponding pOp6 promoter. Upon induction, the LhGR activator binds to the pOp6 promoter and induces expression of the target gene of interest. We provide detailed protocols for inducing transgene expression at different developmental stages and in different plant species and discuss dexamethasone stability and use of its analogs. We also introduce new, versatile, GATEWAY-compatible binary vectors that are now available for the pOp6/LhGR system. © 2019 by John Wiley & Sons, Inc.
CEITEC MU Masaryk University Brno Czech Republic
Department of Plant Sciences University of Oxford Oxford United Kingdom
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