In Situ In Vivo radiolabeling of polymer-coated hydroxyapatite nanoparticles to track their biodistribution in mice
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
30954015
DOI
10.1016/j.colsurfb.2019.03.057
PII: S0927-7765(19)30206-1
Knihovny.cz E-resources
- Keywords
- Bone-seeking agent, Hydroxyapatite nanoparticle, Hydroxybisphosphonate, Polyoxazoline, Radiopharmaceutical,
- MeSH
- Endocytosis MeSH
- Fluorescein chemistry MeSH
- Fluorescent Dyes chemistry MeSH
- Durapatite chemistry MeSH
- Tomography, Emission-Computed, Single-Photon MeSH
- Humans MeSH
- Mice, Inbred BALB C MeSH
- Cell Line, Tumor MeSH
- Nanoparticles chemistry ultrastructure MeSH
- Organotechnetium Compounds chemistry MeSH
- Tomography, X-Ray Computed MeSH
- Polymers chemistry MeSH
- Proton Magnetic Resonance Spectroscopy MeSH
- Radiopharmaceuticals chemistry MeSH
- Tissue Distribution MeSH
- Imaging, Three-Dimensional MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fluorescein MeSH
- Fluorescent Dyes MeSH
- Durapatite MeSH
- Organotechnetium Compounds MeSH
- Polymers MeSH
- Radiopharmaceuticals MeSH
The imaging of healthy tissues and solid tumors benefits from the application of nanoparticle probes with altered pharmacokinetics, not available to low molecular weight compounds. However, the distribution and accumulation of nanoprobes in vivo typically take at least tens of hours to be efficient. For nanoprobes bearing a radioactive label, this is contradictory to the requirement of minimizing the radiation dose for patients by using as-short-as-feasible half-life radionuclides in diagnostics. Thus, we developed a two-stage diagnostic concept for monitoring long-lasting targeting effects with short-lived radioactive labels using bone-mimicking biocompatible polymer-coated and colloidally fully stabilized hydroxyapatite nanoparticles (HAP NPs) and bone-seeking radiopharmaceuticals. Within the pretargeting stage, the nonlabeled nanoparticles are allowed to circulate in the blood. Afterward, 99mTc-1-hydroxyethylidene-1.1-diphosphonate (99mTc-HEDP) is administered intravenously for in situ labeling of the nanoparticles and subsequent single-photon emission computed tomography/computed tomography (SPECT/CT) visualization. The HAP NPs, stabilized with tailored hydrophilic polymers, are not cytotoxic in vitro, as shown by several cell lines. The polymer coating prolongs the circulation of HAP NPs in the blood. The nanoparticles were successfully labeled in vivo with 99mTc-HEDP, 1 and 24 h after injection, and they were visualized by SPECT/CT over time in healthy mice.
References provided by Crossref.org
Radioactive polymeric nanoparticles for biomedical application
