The scaffolds made of polycaprolactone (PCL) are actively employed in different areas of biology and medicine, especially in tissue engineering. However, the usage of unmodified PCL is significantly restricted by the hydrophobicity of its surface, due to the fact that its inert surface hinders the adhesion of cells and the cell interactions on PCL surface. In this work, the surface of PCL nanofibers is modified by Ar/CO₂/C₂H₄ plasma depositing active COOH groups in the amount of 0.57 at % that were later used for the immobilization of platelet-rich plasma (PRP). The modification of PCL nanofibers significantly enhances the viability and proliferation (by hundred times) of human mesenchymal stem cells, and decreases apoptotic cell death to a normal level. According to X-ray photoelectron spectroscopy (XPS), after immobilization of PRP, up to 10.7 at % of nitrogen was incorporated into the nanofibers surface confirming the grafting of proteins. Active proliferation and sustaining the cell viability on nanofibers with immobilized PRP led to an average number of cells of 258 ± 12.9 and 364 ± 34.5 for nanofibers with ionic and covalent bonding of PRP, respectively. Hence, our new method for the modification of PCL nanofibers with PRP opens new possibilities for its application in tissue engineering.

CEITEC Central European Institute of Technology Brno University of Technology Technická 3058 10 61600 Brno Czech Republic

Chair of Physical Metallurgy and Materials Technology Brandenburg Technical University 03046 Cottbus Germany

Institute of Physical Engineering Brno University of Technology Technicka 2896 2 616 69 Brno Czech Republic

National University of Science and Technology MISiS Leninsky pr 4 119049 Moscow Russia

Research Institute of Biochemistry 2 Timakova str 630117 Novosibirsk Russia

RG Plasma Technologies CEITEC⁻Central European Institute of Technology Masaryk University Purkyňova 123 61200 Brno Czech Republic

Scientific Institute of Clinical and Experimental Lymphology Branch of the ICG SB RAS 2 Timakova str 630060 Novosibirsk Russia

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