Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs.

Central European Institute of Technology CEITEC Brno University of Technology Purkyňova 123 Brno 612 00 Czech Republic

Core Facility Nanobiotechnology Central European Institute of Technology CEITEC Masaryk University Kamenice 5 Brno 625 00 Czech Republic

Department of Experimental Biology Faculty of Science Masaryk University Kamenice 5 Brno 625 00 Czech Republic

Department of Physical Electronics Faculty of Science Masaryk University Kotlářská 2 Brno 611 37 Czech Republic

Institute of Physiology of the Czech Academy of Sciences Vídeňská 1083 Prague 142 20 Czech Republic

Research Institute of Clinical and Experimental Lymphology Branch of the ICG SB RAS 2 Timakova str 630060 Novosibirsk Russian Federation

RG Plasma Technologies Central European Institute of Technology CEITEC Masaryk University Kamenice 5 Brno 625 00 Czech Republic

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Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies