Enucleolation and nucleolus transfer in mammalian oocytes and zygotes
Language English Country Spain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
31058302
DOI
10.1387/ijdb.190002mb
PII: 190002mb
Knihovny.cz E-resources
- MeSH
- Cell Nucleolus metabolism transplantation MeSH
- Micromanipulation methods MeSH
- Mice MeSH
- Oocytes cytology drug effects MeSH
- Parthenogenesis MeSH
- Swine MeSH
- Animals MeSH
- Zygote cytology MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.
References provided by Crossref.org
Dynamics and necessity of SIRT1 for maternal-zygotic transition
Improving the Quality of Oocytes with the Help of Nucleolotransfer Therapy
