Enucleolation and nucleolus transfer in mammalian oocytes and zygotes
Jazyk angličtina Země Španělsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
PubMed
31058302
DOI
10.1387/ijdb.190002mb
PII: 190002mb
Knihovny.cz E-zdroje
- MeSH
- buněčné jadérko metabolismus transplantace MeSH
- mikromanipulace metody MeSH
- myši MeSH
- oocyty cytologie účinky léků MeSH
- partenogeneze MeSH
- prasata MeSH
- zvířata MeSH
- zygota cytologie MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.
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