Development of lymphoid progenitors requires a coordinated regulation of gene expression, DNA replication, and gene rearrangement. Chromatin-remodeling activities directed by SWI/SNF2 superfamily complexes play important roles in these processes. In this study, we used a conditional knockout mouse model to investigate the role of Smarca5, a member of the ISWI subfamily of such complexes, in early lymphocyte development. Smarca5 deficiency results in a developmental block at the DN3 stage of αβ thymocytes and pro-B stage of early B cells at which the rearrangement of Ag receptor loci occurs. It also disturbs the development of committed (CD73+) γδ thymocytes. The αβ thymocyte block is accompanied by massive apoptotic depletion of β-selected double-negative DN3 cells and premitotic arrest of CD4/CD8 double-positive cells. Although Smarca5-deficient αβ T cell precursors that survived apoptosis were able to undergo a successful TCRβ rearrangement, they exhibited a highly abnormal mRNA profile, including the persistent expression of CD44 and CD25 markers characteristic of immature cells. We also observed that the p53 pathway became activated in these cells and that a deficiency of p53 partially rescued the defect in thymus cellularity (in contrast to early B cells) of Smarca5-deficient mice. However, the activation of p53 was not primarily responsible for the thymocyte developmental defects observed in the Smarca5 mutants. Our results indicate that Smarca5 plays a key role in the development of thymocytes undergoing β-selection, γδ thymocytes, and also B cell progenitors by regulating the transcription of early differentiation programs.

3rd Faculty of Medicine Charles University Prague 10000 Czech Republic

BIOCEV 1st Faculty of Medicine Charles University Vestec 25250 Czech Republic

BIOCEV 1st Faculty of Medicine Charles University Vestec 25250 Czech Republic;

Czech Centre for Phenogenomics Institute of Molecular Genetics Czech Academy of Sciences Vestec 25250 Czech Republic; and

Department of Cell Biology Albert Einstein College of Medicine Bronx 10461 NY

Institute of Experimental Medicine Czech Academy of Sciences Prague 14220 Czech Republic

Institute of Pathological Physiology 1st Faculty of Medicine Charles University Prague 12853 Czech Republic

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SMARCA5-mediated chromatin remodeling is required for germinal center formation

Differential requirements for Smarca5 expression during hematopoietic stem cell commitment

Loss of ISWI ATPase SMARCA5 (SNF2H) in Acute Myeloid Leukemia Cells Inhibits Proliferation and Chromatid Cohesion