Effect of silver nanoparticles conjugated to thiosemicarbazide on biofilm formation and expression of intercellular adhesion molecule genes, icaAD, in Staphylococcus aureus
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31114932
DOI
10.1007/s12223-019-00715-1
PII: 10.1007/s12223-019-00715-1
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny genetika MeSH
- biofilmy účinky léků MeSH
- kovové nanočástice chemie MeSH
- mikrobiální testy citlivosti MeSH
- molekuly buněčné adheze genetika MeSH
- semikarbazidy farmakologie MeSH
- Staphylococcus aureus účinky léků genetika MeSH
- stříbro farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- molekuly buněčné adheze MeSH
- semikarbazidy MeSH
- stříbro MeSH
- thiosemicarbazide MeSH Prohlížeč
Biofilm formation is regarded as an important factor in the establishment of infections caused by Staphylococcus aureus. In the present study, phenotypic and molecular assays were used to evaluate antibiofilm potential of thiosemicarbazide (Tsc) conjugated with silver nanoparticles (Ag NPs) and functionalized by glutamic acid (Ag@Glu/Tsc NPs) against methicillin-resistant S. aureus (MRSA). Ag NPs were synthesized using precipitation method and conjugated to Tsc using glutamic acid. The NPs were characterized using SEM and FTIR spectroscopy analyses. Then, antibiofilm potential of the prepared NPs against MRSA strains was evaluated using phenotypic method and their effects on the expression of biofilm-associated genes icaA and icaD. Finally, the genes involved with the synthesis of intercellular adhesion molecules were determined. According to the results, Ag@Glu/Tsc NPs inhibited biofilm formation of MRSA strains up to 76.7% compared with the control. In addition, expression of the biofilm-associated genes icaA and icaD reduced by 66.7% and 60.3%, respectively in the presence of sub-inhibitory concentration of Ag@Glu/Tsc NPs. In conclusion, Ag@Glu/Tsc NPs could be considered as a potent antibacterial agent to inhibit bacterial biofilms.
Department of Biology Faculty of Science University of Guilan Rasht Iran
Department of Biology Rasht Branch Islamic Azad University Rasht Iran
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