Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
17-31945A
Ministry of Health of the Czech Republic - International
RVO:68081715
Czech Academy of Sciences - International
LQ1601
Central European Institute of Technology - International
RVO:68081715
Akademie Věd České Republiky - International
17-31945A
Ministerstvo Zdravotnictví Ceské Republiky - International
- Keywords
- Capillary electrophoresis, Contactless conductivity detection, Dual detection, Laser induced fluorescence detection, Saliva,
- MeSH
- Breath Tests methods MeSH
- Equipment Design MeSH
- Electric Conductivity MeSH
- Electrophoresis, Capillary methods MeSH
- Spectrometry, Fluorescence methods MeSH
- Glutathione analysis isolation & purification MeSH
- Ions analysis isolation & purification MeSH
- Carboxylic Acids analysis isolation & purification MeSH
- Humans MeSH
- Limit of Detection MeSH
- Linear Models MeSH
- Reproducibility of Results MeSH
- Tears chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glutathione MeSH
- Ions MeSH
- Carboxylic Acids MeSH
In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C4 D-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C4 D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.
Department of Applied Chemistry Aichi Institute of Technology Toyota Japan
Department of Bioanalytical Instrumentation CEITEC Masaryk University Brno Czech Republic
Department of Chemistry Masaryk University Brno Czech Republic
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