Evaluation of genetic variability of wild hops (Humulus lupulus L.) in Canada and the Caucasus region by chemical and molecular methods
Jazyk angličtina Země Kanada Médium print
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
20616876
DOI
10.1139/g10-024
PII: g10-024
Knihovny.cz E-zdroje
- MeSH
- cyklohexanony metabolismus MeSH
- fylogeneze MeSH
- Humulus klasifikace genetika metabolismus MeSH
- minisatelitní repetice genetika MeSH
- místa se sekvenční adresou * MeSH
- polymorfismus genetický genetika MeSH
- systém (enzymů) cytochromů P-450 metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Kanada MeSH
- Rusko MeSH
- Názvy látek
- cohumulone MeSH Prohlížeč
- colupulone MeSH Prohlížeč
- cyklohexanony MeSH
- systém (enzymů) cytochromů P-450 MeSH
Wild hops (Humulus lupulus L.) are potential new germplasms to expand the variability of genetic resources for hop breeding. We evaluated Canadian (62 plants) and Caucasian (58 plants) wild hops by their chemical characteristics and with molecular genetic analyses using sequence-tagged site and simple sequence repeat markers, in comparison with European (104 plants) and North American (27 plants) wild hops. The contents of alpha and beta acids varied from 0.36% to 5.11% and from 0.43% to 6.66% in Canadian wild hops, and from 0.85% to 3.65% and from 1.22% to 4.81% in Caucasian wild hops, respectively. The contents of cohumulone and colupulone distinctly differed between European and North American wild hops: the cohumulone level in alpha acids was in the range 46.1%-68.4% among North American wild hops and in the range 13.6%-30.6% among European wild hops. The high content of myrcene and the low contents of humulene, farnesene, and selinenes were typical for wild hops from Canada, in contrast to wild hops from the Caucasus region. We compared the chemical characteristics with molecular genetic data. Chemical characteristics differentiated wild hops into North American and Eurasian groups. Molecular genetic analysis was able to separate Caucasian wild hops from European wild hops. We proved a hop phylogeny by means of wide molecular analysis.
Citace poskytuje Crossref.org
Two fingerprinting sets for Humulus lupulus based on KASP and microsatellite markers