Detection and characterization of free oxygen radicals induced protein adduct formation in differentiating macrophages
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
36775000
DOI
10.1016/j.bbagen.2023.130324
PII: S0304-4165(23)00022-3
Knihovny.cz E-resources
- Keywords
- All-trans retinoic acid, Lipid peroxidation, Lipopolysaccharide, Macrophage, Malondialdehyde, Phorbol 12-myristate 13-acetate, Protein oxidation, Reactive oxygen species,
- MeSH
- Electron Spin Resonance Spectroscopy methods MeSH
- Macrophages * metabolism MeSH
- Proteins * chemistry MeSH
- Reactive Oxygen Species metabolism MeSH
- Spin Trapping methods MeSH
- Free Radicals analysis metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Proteins * MeSH
- Reactive Oxygen Species MeSH
- Free Radicals MeSH
Reactive oxygen species play a key role in cellular homeostasis and redox signaling at physiological levels, where excessive production affects the function and integrity of macromolecules, specifically proteins. Therefore, it is important to define radical-mediated proteotoxic stress in macrophages and identify target protein to prevent tissue dysfunction. A well employed, THP-1 cell line was utilized as in vitro model to study immune response and herein we employ immuno-spin trapping technique to investigate radical-mediated protein oxidation in macrophages. Hydroxyl radical formation along macrophage differentiation was confirmed by electron paramagnetic resonance along with confocal laser scanning microscopy using hydroxyphenyl fluorescein. Lipid peroxidation product, malondialdehyde, generated under experimental conditions as detected using swallow-tailed perylene derivative fluorescence observed by confocal laser scanning microscopy and high-performance liquid chromatography, respectively. The results obtained from this study warrant further corroboration and study of specific proteins involved in the macrophage activation and their role in inflammations.
References provided by Crossref.org
NADPH oxidase-dependent free radical generation and protein adduct formation in neutrophils
ROS signaling in innate immunity via oxidative protein modifications
