Development of a CE-MS method for the study of riociguat and metabolite M1 in pharmaceutical analysis
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
CZ.02.1.01/0.0/0.0/15_003/0000417
Ministry of Education, Youth and Sports - International
PubMed
31520473
DOI
10.1002/elps.201900181
Knihovny.cz E-zdroje
- Klíčová slova
- Capillary electrophoresis, Dissociation constant, Fragmentation, Mass spectrometry, Riociguat,
- MeSH
- elektroforéza kapilární metody MeSH
- hmotnostní spektrometrie metody MeSH
- lidé MeSH
- limita detekce MeSH
- lineární modely MeSH
- pyrazoly analýza krev chemie metabolismus MeSH
- pyrimidiny analýza krev chemie metabolismus MeSH
- reprodukovatelnost výsledků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- pyrazoly MeSH
- pyrimidiny MeSH
- riociguat MeSH Prohlížeč
Riociguat is a novel antihypertensive drug for the treatment of pulmonary hypertension. We present electrophoretic characterization, i.e. migration behavior of riociguat and metabolite M1 as support for optimized CZE/MS assay. Fundamental separation parameters, such as peak width, symmetry, and resolution are studied in a series of ammonium formate buffers within pH range 2.60-5.61. The narrow region of peak symmetry lies close to pH 4.0 for both analytes. Accordingly, the value of resolution maximizes in a background electrolyte adjusted to pH 4.10. Basic calibration parameters estimated from CZE experiments with absorption photometric and mass spectrometric detection of riociguat and metabolite M1 were evaluated. More than three orders lower LOD was achieved with high resolution mass spectrometric detection. The observed difference in the sensitivity of both detection techniques gives priority to the utilization of CZE/MS in practice. The values of dissociation constants of riociguat and metabolite M1, pKBH , were determined from CZE measurements in lithium formate and lithium acetate background electrolytes with constant ionic strength. The value of pKBH = 4.30 ± 0.02 for riociguat corresponds well to the value already presented in the literature. According to our observation, metabolite M1 behaves like a slightly stronger base with estimated pKBH = 4.40 ± 0.02.
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