γ-Tubulin interacts with E2F transcription factors to regulate proliferation and endocycling in Arabidopsis
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
BB/M025047/1
Biotechnology and Biological Sciences Research Council - United Kingdom
PubMed
31693141
DOI
10.1093/jxb/erz498
PII: 5613973
Knihovny.cz E-zdroje
- Klíčová slova
- Arabidopsis, E2F transcription factors, endoreduplication, gene expression, proliferation, γ-tubulin,
- MeSH
- Arabidopsis * genetika metabolismus MeSH
- proteiny buněčného cyklu MeSH
- proteiny huseníčku * genetika metabolismus MeSH
- regulace genové exprese u rostlin MeSH
- transkripční faktory E2F * genetika metabolismus MeSH
- tubulin * genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- E2Fb protein, Arabidopsis MeSH Prohlížeč
- proteiny buněčného cyklu MeSH
- proteiny huseníčku * MeSH
- transkripční faktory E2F * MeSH
- tubulin * MeSH
γ-Tubulin is associated with microtubule nucleation, but evidence is accumulating in eukaryotes that it also functions in nuclear processes and in cell division control independently of its canonical role. We found that in Arabidopsis thaliana, γ-tubulin interacts specifically with E2FA, E2FB, and E2FC transcription factors both in vitro and in vivo. The interaction of γ-tubulin with the E2Fs is not reduced in the presence of their dimerization partners (DPs) and, in agreement, we found that γ-tubulin interaction with E2Fs does not require the dimerization domain. γ-Tubulin associates with the promoters of E2F-regulated cell cycle genes in an E2F-dependent manner, probably in complex with the E2F-DP heterodimer. The up-regulation of E2F target genes PCNA, ORC2, CDKB1;1, and CCS52A under γ-tubulin silencing suggests a repressive function for γ-tubulin at G1/S and G2/M transitions, and the endocycle, which is consistent with an excess of cell division in some cells and enhanced endoreduplication in others in the shoot and young leaves of γ-tubulin RNAi plants. Altogether, our data show ternary interaction of γ-tubulin with the E2F-DP heterodimer and suggest a repressive role for γ-tubulin with E2Fs in controlling mitotic activity and endoreduplication during plant development.
Institute of Microbiology of the Czech Academy of Sciences Vídeňská Czech Republic
Institute of Molecular Genetics of the Czech Academy of Sciences Vídeňská Czech Republic
Institute of Plant Biology Biological Research Centre Szeged Hungary
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