miR-181a-2* expression is different amongst carcinomas from the colorectal serrated route
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31784758
DOI
10.1093/mutage/gez039
PII: 5648152
Knihovny.cz E-resources
- MeSH
- CpG Islands MeSH
- Cytokines genetics metabolism MeSH
- TNF Receptor-Associated Factor 1 genetics metabolism MeSH
- Gene Ontology MeSH
- Carcinoma genetics metabolism physiopathology MeSH
- Colorectal Neoplasms genetics metabolism physiopathology MeSH
- Humans MeSH
- DNA Methylation MeSH
- MicroRNAs genetics metabolism MeSH
- Microsatellite Instability * MeSH
- Cell Line, Tumor MeSH
- Nicotinamide Phosphoribosyltransferase genetics metabolism MeSH
- Gene Expression Regulation, Neoplastic genetics MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Aged MeSH
- Transcription Factors genetics metabolism MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokines MeSH
- TNF Receptor-Associated Factor 1 MeSH
- MicroRNAs MeSH
- MIrn181 microRNA, human MeSH Browser
- nicotinamide phosphoribosyltransferase, human MeSH Browser
- Nicotinamide Phosphoribosyltransferase MeSH
- SALL1 protein, human MeSH Browser
- Transcription Factors MeSH
Serrated adenocarcinoma (SAC) and colorectal carcinomas showing histological and molecular features of high-level of microsatellite instability (hmMSI-H) are both end points of the serrated pathway of colorectal carcinogenesis. Despite common features (right-sided location, CpG island methylation phenotype and BRAF mutation) there are no studies comparing the microRNA (miRNA) expression profiles in SACs and hmMSI-H. The microtranscriptome from 12 SACs and 8 hmMSI-H were analysed using Affymetrix GeneChip miRNA 3.0 arrays and differentially enriched functions involving immune response were observed from this comparison. miR-181a-2* was found significantly more expressed in hmMSI-H than in SAC and higher expression of this miRNA in microsatellite unstable colorectal cancer were corroborated by Real-Time PCR in an extended series (61 SAC, 21 hmMSI-H). An analysis of genes possibly regulated by miR-181a-2* was carried out and, amongst these, an inverse correlation of NAMPT with miR-181a-2* expression was observed, whereas, for TRAF1 and SALL1, additional regulation mechanisms involving CpG island methylation were observed. miR-181a-2* is associated with particular histological and molecular features of colorectal carcinomas within the serrated pathological pathway and might play a role in the immune responses of microsatellite instability carcinomas.
Central European Institute of Technology Masaryk University Brno Czech Republic
Clinical Analysis Department Santa Lucia University Hospital Cartagena Spain
Department of Surgery University Hospital Brno Brno Bohunice Brno Starý Lískovec Czech Republic
Pathology Department Santa Lucia University Hospital Cartagena Spain
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