Root cultures of potato mutant lacking MSPI isoform, indispensable for photosynthetic light reactions, exhibit characteristics similar to intact plant roots
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31887556
DOI
10.1016/j.jplph.2019.153091
PII: S0176-1617(19)30220-2
Knihovny.cz E-zdroje
- Klíčová slova
- Carbohydrate allocation, In vitro cultivation, PsbO1 gene, Root branching,
- MeSH
- alely MeSH
- fotosyntéza genetika účinky záření MeSH
- fotosystém II (proteinový komplex) genetika metabolismus MeSH
- hlízy rostlin genetika růst a vývoj MeSH
- kořeny rostlin růst a vývoj metabolismus MeSH
- kultivované buňky MeSH
- mangan metabolismus MeSH
- metabolismus sacharidů genetika MeSH
- mutace MeSH
- mutantní proteiny chemie genetika metabolismus MeSH
- protein - isoformy genetika metabolismus MeSH
- regulace genové exprese u rostlin genetika fyziologie MeSH
- rostlinné proteiny genetika metabolismus MeSH
- sacharosa metabolismus MeSH
- Solanum tuberosum genetika růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fotosystém II (proteinový komplex) MeSH
- mangan MeSH
- mutantní proteiny MeSH
- protein - isoformy MeSH
- rostlinné proteiny MeSH
- sacharosa MeSH
Potato (Solanum tuberosum) mutant (ST) lacking one isoform of manganese-stabilizing protein (MSPI) of photosystem II exhibited besides spontaneous tuberization also growth changes with strongly impaired root system development. Previous studies revealed marked changes in carbohydrate levels and allocation within ST plant body. To verify causal relationship between changed carbohydrate balance and root growth restriction we engaged dark grown sucrose-supplied root organ-cultures of ST plants to exclude/confirm shoot effects. Unexpectedly, in ST root cultures we observed large alterations in growth and architecture as well as saccharide status similar to those found in the intact plant roots. The gene expression analysis, however, proved PsbO1 transcript (coding MSPI protein) neither in ST nor in WT root-organ cultures. Therefore, the results point to indirect effects of PsbO1 allele absence connected possibly with some epigenetic modulations.
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