The photosynthetic machinery of plants can acclimate to changes in light conditions by balancing light-harvesting between the two photosystems (PS). This acclimation response is induced by the change in the redox state of the plastoquinone pool, which triggers state transitions through activation of the STN7 kinase and subsequent phosphorylation of light-harvesting complex II (LHCII) proteins. Phosphorylation of LHCII results in its association with PSI (state 2), whereas dephosphorylation restores energy allocation to PSII (state 1). In addition to state transition regulation by phosphorylation, we have recently discovered that plants lacking the chloroplast acetyltransferase NSI are also locked in state 1, even though they possess normal LHCII phosphorylation. This defect may result from decreased lysine acetylation of several chloroplast proteins. Here, we compared the composition of wild type (wt), stn7 and nsi thylakoid protein complexes involved in state transitions separated by Blue Native gel electrophoresis. Protein complex composition and relative protein abundances were determined by LC-MS/MS analyses using iBAQ quantification. We show that despite obvious mechanistic differences leading to defects in state transitions, no major differences were detected in the composition of PSI and LHCII between the mutants. Moreover, both stn7 and nsi plants show retarded growth and decreased PSII capacity under fluctuating light as compared to wt, while the induction of non-photochemical quenching under fluctuating light was much lower in both nsi mutants than in stn7.

Centre Algatech Institute of Microbiology Czech Academy of Sciences Novohradská 237 Opatovický mlýn 379 81 Třebon Czech Republic

Compact Plants Phenomics Center Washington State University Pullman WA 99164 USA

Department of Biochemistry Molecular Plant Biology University of Turku Biocity A Tykistökatu 6 20520 Turku Finland

Institute of Biological Chemistry Washington State University Pullman WA 99164 6340 USA

Plant Physiology Institute of Plant Biology and Biotechnology University of Münster Schlossplatz 7 48149 Münster Germany

Plant Physiology School of Biological Sciences Washington State University Pullman WA 99164 4236 USA

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