The main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological processes such as immunity and development throughout the metazoan. Herein, three visual artists and a music professor collaborated with scientists for the functional characterization of the AKR2 interactome in the regulation of the NF-κB pathway in human placenta cells. The results served as a methodological proof-of-concept to advance this research area. The results showed new perspectives on unexplored characteristics of AKR2 with functional implications. These results included protein dimerization, the physical interactions with different proteins simultaneously to regulate various biological processes defined by cell type-specific AKR-protein interactions, and how these interactions positively or negatively regulate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway in a biological context-dependent manner. These results suggested that AKR2-interacting proteins might constitute suitable secondary transcription factors for cell- and stimulus-specific regulation of NF-κB. Musical perspective supported AKR/SUB evolutionary conservation in different species and provided new mechanistic insights into the AKR2 interactome. The combined scientific and artistic perspectives resulted in a multidisciplinary approach, advancing our knowledge on AKR/SUB interactome, and provided new insights into the function of AKR2-protein interactions in the regulation of the NF-κB pathway. Additionally, herein we proposed an algorithm for quantum vaccinomics by focusing on the model proteins AKR/SUB.

Artesadhana Studio Mérida 97000 Mexico

Centro de Investigación y Documentación Musical CIDoM UCLM CSIC Facultad de Educación de Ciudad Real Ronda Calatrava 3 13071 Ciudad Real Spain

Department of Veterinary Pathobiology Center for Veterinary Health Sciences Oklahoma State University Stillwater OK 74078 USA

Department of Virology Veterinary Research Institute Hudcova 70 62100 Brno Czech Republic

Facultad de Veterinaria Universidad de Zaragoza 50013 Zaragoza Spain

Faculty of Science University of South Bohemia 37005 České Budějovice Czech Republic

Institute of Parasitology Biology Centre Czech Academy of Sciences Branišovská 1160 31 37005 České Budějovice Czech Republic

León Viera Studio Calle 60 No 338 M por 31 Colonia Alcalá Martín Mérida 97000 Mexico

Raúl Cordero Studio Calle Rio Elba 21 8 Colonia Cuauhtémoc CDMX 06500 Mexico

SaBio Instituto de Investigación en Recursos Cinegéticos IREC CSIC UCLM JCCM Ronda de Toledo s n 13005 Ciudad Real Spain

UMR BIPAR INRA ANSES Ecole Nationale Vétérinaire d'Alfort Université Paris Est Maisons Alfort 94700 France

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Vaccines (Basel). 2020 Jul 25;8(3):E418. doi: 10.3390/vaccines8030418. PubMed

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Modelling protein-protein interactions for the design of vaccine chimeric antigens with protective epitopes