A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin
Jazyk angličtina Země Anglie, Velká Británie Médium electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
32157102
PubMed Central
PMC7064611
DOI
10.1038/s41598-020-61280-z
PII: 10.1038/s41598-020-61280-z
Knihovny.cz E-zdroje
- MeSH
- bilirubin analogy a deriváty krev chemie MeSH
- chromatografie kapalinová MeSH
- fototerapie metody MeSH
- lidé MeSH
- molekulární struktura MeSH
- novorozenec MeSH
- novorozenecká žloutenka krev terapie MeSH
- sérum chemie MeSH
- tandemová hmotnostní spektrometrie MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bilirubin MeSH
- lumirubin MeSH Prohlížeč
Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p < 0.01), but the absolute concentrations were low (0.37 ± 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 ± 2.2 vs. 10.3 ± 3.3 mg/dL, p < 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.
Department of Chemistry and Recetox Faculty of Science Masaryk University Brno Czech Republic
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Bhutani VK, et al. Noninvasive measurement of total serum bilirubin in a multiracial predischarge newborn population to assess the risk of severe hyperbilirubinemia. Pediatrics. 2000;106:E17. doi: 10.1542/peds.106.2.e17. PubMed DOI
Fouzas S, Mantagou L, Skylogianni E, Mantagos S, Varvarigou A. Transcutaneous bilirubin levels for the first 120 postnatal hours in healthy neonates. Pediatrics. 2010;125:e52–57. doi: 10.1542/peds.2009-0403. PubMed DOI
Newman TB, Easterling MJ, Goldman ES, Stevenson DK. Laboratory evaluation of jaundice in newborns; Frequency,cost, yield. Am. J. Dis. Child. 1990;144:364–368. doi: 10.1001/archpedi.1990.02150270114039. PubMed DOI
Cremer RJ, Perryman PW, Richards DH. Influence of light on the hyperbilirubinemia of infants. Lancet. 1958;i:1094–1097. doi: 10.1016/S0140-6736(58)91849-X. PubMed DOI
Vreman HJ, Wong RJ, Stevenson DK. Phototherapy: current methods and future directions. Semin. Perinatol. 2004;28:326–333. doi: 10.1053/j.semperi.2004.09.003. PubMed DOI
Maisels MJ, McDonagh AF. Phototherapy for neonatal jaundice. N. Engl. J. Med. 2008;358:920–928. doi: 10.1056/NEJMct0708376. PubMed DOI
Tyson JE, et al. Does aggressive phototherapy increase mortality while decreasing profound impairment among the smallest and sickest newborns? J. Perinatol. 2012;32:677–684. doi: 10.1038/jp.2012.64. PubMed DOI PMC
Arnold C, Pedroza C, Tyson JE. Phototherapy in ELBW newborns: does it work? Is it safe? The evidence from randomized clinical trials. Semin. Perinatol. 2014;38:452–464. doi: 10.1053/j.semperi.2014.08.008. PubMed DOI
Morris BH, et al. Aggressive vs. conservative phototherapy for infants with extremely low birth weight. N. Engl. J. Med. 2008;359:1885–1896. doi: 10.1056/NEJMoa0803024. PubMed DOI PMC
Auger N, Laverdiere C, Ayoub A, Lo E, Luu TM. Neonatal phototherapy and future risk of childhood cancer. Int. J. Cancer. 2019;145:2061–2069. doi: 10.1002/ijc.32158. PubMed DOI
Ramy N, et al. Jaundice, phototherapy and DNA damage in full-term neonates. J. Perinatol. 2016;36:132–136. doi: 10.1038/jp.2015.166. PubMed DOI
Bonnett R, et al. Photobilirubin II. Biochem. J. 1984;219:1053–1056. doi: 10.1042/bj2191053. PubMed DOI PMC
Stoll MS, Vicker N, Gray CH, Bonnett R. Concerning the structure of photobilirubin II. Biochem. J. 1982;201:179–188. doi: 10.1042/bj2010179. PubMed DOI PMC
Stoll MS, Zenone EA, Ostrow JD, Zarembo JE. Preparation and properties of bilirubin photoisomers. Biochem. J. 1979;183:139–146. doi: 10.1042/bj1830139. PubMed DOI PMC
McDonagh AF. Bilirubin photo-isomers: regiospecific acyl glucuronidation in vivo. Monatsh Chem. 2014;145:465–482. doi: 10.1007/s00706-013-1076-6. DOI
McDonagh AF, Palma LA, Trull FR, Lightner DA. Phototherapy for neonatal jaundice. Configurational isomers of bilirubin. J. Am. Chem. Soc. 1982;104:6865–6869. doi: 10.1021/ja00388a103. DOI
Itoh S, Isobe K, Onishi S. Accurate and sensitive high-performance liquid chromatographic method for geometrical and structural photoisomers of bilirubin IX alpha using the relative molar absorptivity values. J. Chromatogr. A. 1999;848:169–177. doi: 10.1016/S0021-9673(99)00469-0. PubMed DOI
McDonagh AF, Assisi F. The ready isomerization of bilirubin IX- in aqueous solution. Biochem. J. 1972;129:797–800. doi: 10.1042/bj1290797. PubMed DOI PMC
Jašprová J, et al. Neuro-inflammatory effects of photodegradative products of bilirubin. Sci. Rep. 2018;8:7444. doi: 10.1038/s41598-018-25684-2. PubMed DOI PMC
Folch J, Lees M, Stanley GHS. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 1957;226:497–509. PubMed
Zelenka J, et al. Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues. J. Chrom B Anal. Technol. Biomed. Life Sci. 2008;867:37–42. doi: 10.1016/j.jchromb.2008.03.005. PubMed DOI
Vecka, M. et al. Comprehensive sterol and fatty acid analysis in nineteen nuts, seeds, and kernel. SN Applied Sciences1, 10.1007/s42452-019-1576-z (2019).
Management of hyperbilirubinemia in the newborn infant 35 or more weeks of gestation. Pediatrics114, 297–316 (2004). PubMed
Jasprova J, et al. The biological effects of bilirubin photoisomers. PLoS One. 2016;11:e0148126. doi: 10.1371/journal.pone.0148126. PubMed DOI PMC
Soares DG, Andreazza AC, Salvador M. Sequestering ability of butylated hydroxytoluene, propyl gallate, resveratrol, and vitamins C and E against ABTS, DPPH, and hydroxyl free radicals in chemical and biological systems. J. Agric. Food Chem. 2003;51:1077–1080. doi: 10.1021/jf020864z. PubMed DOI
Reddy AC, Lokesh BR. Studies on spice principles as antioxidants in the inhibition of lipid peroxidation of rat liver microsomes. Mol. Cell Biochem. 1992;111:117–124. PubMed
Ebbesen F, et al. Bilirubin isomer distribution in jaundiced neonates during phototherapy with LED light centered at 497 nm (turquoise) vs. 459 nm (blue) Pediatr. Res. 2016;80:511–515. doi: 10.1038/pr.2016.115. PubMed DOI
McDonagh AF. Light effects on transport and excretion of bilirubin in newborns. Ann. NY. Acad. Sci. 1985;453:65–72. doi: 10.1111/j.1749-6632.1985.tb11798.x. PubMed DOI
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