PCR Detection of Oxacillinases in Bacteria
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
32212994
DOI
10.1089/mdr.2019.0330
Knihovny.cz E-resources
- Keywords
- PCR, antibiotic resistance, oxacillinases, primers,
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Bacterial Proteins genetics metabolism MeSH
- beta-Lactamases genetics metabolism MeSH
- DNA Primers chemical synthesis metabolism MeSH
- Enterobacter cloacae classification drug effects enzymology genetics MeSH
- Escherichia coli classification drug effects enzymology genetics MeSH
- Gene Expression MeSH
- Phylogeny MeSH
- Gram-Negative Bacterial Infections diagnosis epidemiology microbiology veterinary MeSH
- Klebsiella pneumoniae classification drug effects enzymology genetics MeSH
- Chickens microbiology MeSH
- Humans MeSH
- Meat microbiology MeSH
- Microbial Sensitivity Tests MeSH
- Multiplex Polymerase Chain Reaction methods MeSH
- Penicillins pharmacology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Bacterial Proteins MeSH
- beta-Lactamases MeSH
- DNA Primers MeSH
- oxacillinase MeSH Browser
- Penicillins MeSH
Oxacillinases (OXA) have been mostly described in Enterobacteriaceae, Acinetobacter, and Pseudomonas species. Recent years have witnessed an increased prevalence of intrinsic and/or acquired β-lactamase-producing Acinetobacter in food-producing animals. This study was conducted to assess the prevalence of OXA among selected bacterial species and to characterize these enzymes by in silico analysis. Screening of OXA was performed by PCR amplification using specific pairs of oligonucleotides. Overall, 40 pairs of primers were designed, of which 6 were experimentally tested in vitro. Among 49 bacterial isolates examined, the presence of blaOXA-1-like genes was confirmed in 20 cases (41%; 19 times in Klebsiella pneumoniae and once in Enterobacter cloacae). No OXA were found in animal isolates. The study results confirmed the specificity of the designed oligonucleotide pairs. Furthermore, the designed primers were found to possess the ability to specifically detect 90.2% of all OXA. These facts suggest that the in silico and in vitro tested primers could be used for single or multiplex PCR to screen for the presence of OXA in various bacteria, as well as to monitor their spread. At the same time, the presence of conserved characteristic amino acids and motifs was confirmed by in silico analysis of sequences of representative members of OXA.
References provided by Crossref.org
Analysis of BlaEC family class C beta-lactamase
In Silico Analysis of Extended-Spectrum β-Lactamases in Bacteria
