First records raise questions: DNA barcoding of Odonata in the middle of the Mediterranean
Language English Country Canada Media print-electronic
Document type Journal Article
PubMed
32502367
DOI
10.1139/gen-2019-0226
Knihovny.cz E-resources
- Keywords
- BIN, COI, DNA barcode reference library, Malta, Malte, banque de référence de codes à barres de l’ADN, inventaire des espèces, islands, species inventory, îles,
- MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- Electron Transport Complex IV genetics MeSH
- DNA Barcoding, Taxonomic * MeSH
- Odonata classification genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Malta MeSH
- Names of Substances
- Electron Transport Complex IV MeSH
We present the results of the first-ever DNA barcoding study of odonates from the Maltese Islands. In total, 10 morphologically identified species were collected during a two-week long expedition in 2018. Eighty cytochrome c oxidase subunit I (COI) barcodes were obtained from the collected specimens. Intra- and interspecific distances ranged from 0.00% to 2.24% and 0.48% to 17.62%, respectively. Successful species identification based on ascribing a single morphological species to a single Barcode Index Number (BIN) was achieved for eight species (80%). In the case of two species, Ischnura genei and Anax parthenope, BINs were shared with other closely related species. The taxonomic status of I. genei is questionable and the phylogenetic relationship between A. imperator/parthenope is not clear. Further studies involving a series of adult specimens collected in a wide spatial range and nuclear markers are necessary to resolve these cases. Therefore, this dataset serves as an initial DNA barcode reference library for Maltese odonates, within a larger project: Aquatic Macroinvertebrates DNA Barcode Library of Malta.
Malta College of Arts Science and Technology Paola Malta
Masaryk University Department of Botany and Zoology Brno Czechia
University of Guelph Centre for Biodiversity Genomics Guelph Ontario Canada
University of Lodz Department of Invertebrate Zoology and Hydrobiology Łódź Poland
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