A massive systematic infection of Encephalitozoon cuniculi genotype III in mice does not cause clinical signs
Language English Country France Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
32579904
DOI
10.1016/j.micinf.2020.06.004
PII: S1286-4579(20)30122-2
Knihovny.cz E-resources
- Keywords
- Albendazole, C57Bl/6 mice, CD4(−/−) mice, CD8(−/−) mice, Encephalitozoon cuniculi genotype III,
- MeSH
- Albendazole therapeutic use MeSH
- CD4 Antigens genetics MeSH
- CD8 Antigens genetics MeSH
- Chlorocebus aethiops MeSH
- Encephalitozoon cuniculi genetics MeSH
- Encephalitozoonosis drug therapy microbiology pathology MeSH
- Genotype * MeSH
- Disease Models, Animal MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Vero Cells MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Albendazole MeSH
- CD4 Antigens MeSH
- CD8 Antigens MeSH
Encephalitozoon cuniculi genotype III disseminated intensively into most of the organs in all strains of mice, followed by a chronic infection with massive microsporidia persistence in immunodeficient mice and a partial decrease in C57Bl/6 mice. Treatment with 0.2 mg Albendazole/mouse/day temporarily reduces the number of affected organs in immunocompetent C57Bl/6 mice, but not in CD4-/- and CD8-/- mice. The application of medication temporarily decreased the spore burden at least by one order of magnitude in all groups. These results demonstrate that the E. cuniculi genotype III infection had a progressive course and surprisingly, Albendazole treatment had only a minimal effect. The E. cuniculi genotype III spore burden in individual organs reached up to 108 or 109 in immunocompetent or immunodeficient mice, respectively; however, these mice did not demonstrate any obvious clinical signs of microsporidiosis, and the immunodeficient mice survived longer. Our findings clearly show that the survival of mice does not correspond to spore burden, which provides new insight into latent microsporidiosis from an epidemiological point of view.
References provided by Crossref.org
Chronic Infections in Mammals Due to Microsporidia
