Microtubule bundling is an essential mechanism underlying the biased organization of interphase and mitotic microtubular systems of eukaryotes in ordered arrays. Microtubule bundle formation can be exemplified in plants, where the formation of parallel microtubule systems in the cell cortex or the spindle midzone is largely owing to the microtubule crosslinking activity of a family of microtubule associated proteins, designated as MAP65s. Among the nine members of this family in Arabidopsis thaliana, MAP65-1 and MAP65-2 are ubiquitous and functionally redundant. Crosslinked microtubules can form high-order arrays, which are difficult to track using widefield or confocal laser scanning microscopy approaches. Here, we followed spatiotemporal patterns of MAP65-2 localization in hypocotyl cells of Arabidopsis stably expressing fluorescent protein fusions of MAP65-2 and tubulin. To circumvent imaging difficulties arising from the density of cortical microtubule bundles, we use different superresolution approaches including Airyscan confocal laser scanning microscopy (ACLSM), structured illumination microscopy (SIM), total internal reflection SIM (TIRF-SIM), and photoactivation localization microscopy (PALM). We provide insights into spatiotemporal relations between microtubules and MAP65-2 crossbridges by combining SIM and ACLSM. We obtain further details on MAP65-2 distribution by single molecule localization microscopy (SMLM) imaging of either mEos3.2-MAP65-2 stochastic photoconversion, or eGFP-MAP65-2 stochastic emission fluctuations under specific illumination conditions. Time-dependent dynamics of MAP65-2 were tracked at variable time resolution using SIM, TIRF-SIM, and ACLSM and post-acquisition kymograph analysis. ACLSM imaging further allowed to track end-wise dynamics of microtubules labeled with TUA6-GFP and to correlate them with concomitant fluctuations of MAP65-2 tagged with tagRFP. All different microscopy modules examined herein are accompanied by restrictions in either the spatial resolution achieved, or in the frame rates of image acquisition. PALM imaging is compromised by speed of acquisition. This limitation was partially compensated by exploiting emission fluctuations of eGFP which allowed much higher photon counts at substantially smaller time series compared to mEos3.2. SIM, TIRF-SIM, and ACLSM were the methods of choice to follow the dynamics of MAP65-2 in bundles of different complexity. Conclusively, the combination of different superresolution methods allowed for inferences on the distribution and dynamics of MAP65-2 within microtubule bundles of living A. thaliana cells.

Department of Cell Biology Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Olomouc Olomouc Czechia

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Adamowski M., Li L., Friml J. (2019). Reorientation of cortical microtubule arrays in the hypocotyl of PubMed DOI PMC

Bagshaw C. R., Cherny D. (2006). Blinking fluorophores: what do they tell us about protein dynamics? PubMed DOI

Bannigan A., Lizotte-Waniewski M., Riley M., Baskin T. I. (2008). Emerging molecular mechanisms that power and regulate the anastral mitotic spindle of flowering plants. PubMed DOI

Beck M., Komis G., Müller J., Menzel D., Šamaj J. (2010). PubMed DOI PMC

Beck M., Komis G., Ziemann A., Menzel D., Šamaj J. (2011). Mitogen-activated protein kinase 4 is involved in the regulation of mitotic and cytokinetic microtubule transitions in PubMed DOI

Boruc J., Weimer A. K., Stoppin-Mellet V., Mylle E., Kosetsu K., Cedeño C., et al. (2017). Phosphorylation of MAP65-1 by Arabidopsis aurora kinases is required for efficient cell cycle progression. PubMed DOI PMC

Bratman S. V., Chang F. (2007). Stabilization of overlapping microtubules by fission yeast CLASP. PubMed DOI PMC

Burkart G., Dixit R. (2019). Microtubule bundling by MAP65-1 protects against severing by inhibiting the binding of katanin. PubMed DOI PMC

Buschmann H., Sambade A., Pesquet E., Calder G., Lloyd C. W. (2010). “Chapter 20 - microtubule dynamics in plant cells,” in PubMed DOI

Buschmann H., Zachgo S. (2016). The evolution of cell division: from streptophyte algae to land plants. PubMed DOI

Caillaud M.-C., Lecomte P., Jammes F., Quentin M., Pagnotta S., Andrio E., et al. (2008). MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in PubMed DOI PMC

Celler K., Fujita M., Kawamura E., Ambrose C., Herburger K., Holzinger A., et al. (2016). “Microtubules in plant cells: strategies and methods for immunofluorescence, transmission electron microscopy, and live cell imaging,” in PubMed DOI PMC

Chan J., Jensen C. G., Jensen L. C. W., Bush M., Lloyd C. W. (1999). The 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules. PubMed DOI PMC

Chen X., Grandont L., Li H., Hauschild R., Paque S., Abuzeineh A., et al. (2014). Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. PubMed DOI PMC

Chen X., Wu S., Liu Z., Friml J. (2016). Environmental and endogenous control of cortical microtubule orientation. PubMed DOI

Chi Z., Ambrose C. (2016). Microtubule encounter-based catastrophe in Arabidopsis cortical microtubule arrays. PubMed DOI PMC

Costes S. V., Daelemans D., Cho E. H., Dobbin Z., Pavlakis G., Lockett S. (2004). Automatic and quantitative measurement of protein-protein colocalization in live cells. PubMed PMC

Cox S., Rosten E., Monypenny J., Jovanovic-Talisman T., Burnette D. T., Lippincott-Schwartz J., et al. (2012). Bayesian localization microscopy reveals nanoscale podosome dynamics. PubMed DOI PMC

Deinum E. E., Tindemans S. H., Lindeboom J. J., Mulder B. M. (2017). How selective severing by katanin promotes order in the plant cortical microtubule array. PubMed DOI PMC

Demmerle J., Innocent C., North A. J., Ball G., Müller M., Miron E., et al. (2017). Strategic and practical guidelines for successful structured illumination microscopy. PubMed DOI

Derbyshire P., Ménard D., Green P., Saalbach G., Buschmann H., Lloyd C. W., et al. (2015). Proteomic analysis of microtubule interacting proteins over the course of xylem tracheary element formation in Arabidopsis. PubMed DOI PMC

Dertinger T., Colyer R., Iyer G., Weiss S., Enderlein J. (2009). Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI). PubMed DOI PMC

Dixit R., Cyr R. (2004). Encounters between dynamic cortical microtubules promote ordering of the cortical array through angle-dependent modifications of microtubule behavior. PubMed DOI PMC

Dong B., Yang X., Zhu S., Bassham D. C., Fang N. (2015). Stochastic optical reconstruction microscopy imaging of microtubule arrays in intact PubMed DOI PMC

Dunn K. W., Kamocka M. M., McDonald J. H. (2011). A practical guide to evaluating colocalization in biological microscopy. PubMed DOI PMC

Durst S., Hedde P. N., Brochhausen L., Nick P., Nienhaus G. U., Maisch J. (2014). Organization of perinuclear actin in live tobacco cells observed by PALM with optical sectioning. PubMed DOI

Elliott A., Shaw S. L. (2018a). A cycloheximide-sensitive step in transverse microtubule array patterning. PubMed DOI PMC

Elliott A., Shaw S. L. (2018b). Update: plant cortical microtubule arrays. PubMed DOI PMC

Eng R. C., Sampathkumar A. (2018). Getting into shape: the mechanics behind plant morphogenesis. PubMed DOI

Gaillard J., Neumann E., Van Damme D., Stoppin-Mellet V., Ebel C., Barbier E., et al. (2008). Two microtubule-associated proteins of PubMed DOI PMC

Galva C., Kirik V., Lindeboom J. J., Kaloriti D., Rancour D. M., Hussey P. J., et al. (2014). The microtubule plus-end tracking proteins SPR1 and EB1b interact to maintain polar cell elongation and directional organ growth in PubMed DOI PMC

Gardner M. K., Zanic M., Howard J. (2013). Microtubule catastrophe and rescue. PubMed DOI PMC

Hamada T. (2014). “Chapter one - microtubule organization and microtubule-associated proteins in plant cells,” in PubMed DOI

Hamant O., Inoue D., Bouchez D., Dumais J., Mjolsness E. (2019). Are microtubules tension sensors? PubMed DOI PMC

Haupts U., Maiti S., Schwille P., Webb W. W. (1998). Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy. PubMed PMC

Havelková L., Nanda G., Martinek J., Bellinvia E., Sikorová L., Šlajcherová K., et al. (2015). Arp2/3 complex subunit ARPC2 binds to microtubules. PubMed DOI

Ho C.-M. K., Lee Y.-R. J., Kiyama L. D., Dinesh-Kumar S. P., Liu B. (2012). Arabidopsis microtubule-associated protein MAP65-3 cross-links antiparallel microtubules toward their plus ends in the phragmoplast via its distinct C-terminal microtubule binding domain. PubMed DOI PMC

Hoogendoorn E., Crosby K. C., Leyton-Puig D., Breedijk R. M. P., Jalink K., Gadella T. W. J., et al. (2014). The fidelity of stochastic single-molecule super-resolution reconstructions critically depends upon robust background estimation. PubMed DOI PMC

Hosy E., Martiničre A., Choquet D., Maurel C., Luu D.-T. (2015). Super-resolved and dynamic imaging of membrane proteins in plant cells reveal contrasting kinetic profiles and multiple confinement mechanisms. PubMed DOI

Huff J. (2016). The fast mode for ZEISS LSM 880 with airyscan: high-speed confocal imaging with super-resolution and improved signal-to-noise ratio. DOI

Hussey P. J., Hawkins T. J., Igarashi H., Kaloriti D., Smertenko A. (2002). The plant cytoskeleton: recent advances in the study of the plant microtubule-associated proteins MAP-65, MAP-190 and the PubMed DOI

Janson M. E., Loughlin R., Loïodice I., Fu C., Brunner D., Nédélec F. J., et al. (2007). Crosslinkers and motors organize dynamic microtubules to form stable bipolar arrays in fission yeast. PubMed DOI

Jradi F. M., Lavis L. D. (2019). Chemistry of photosensitive fluorophores for single-molecule localization microscopy. PubMed DOI

Kapoor V., Hirst W. G., Hentschel C., Preibisch S., Reber S. (2019). MTrack: automated detection, tracking, and analysis of dynamic microtubules. PubMed DOI PMC

Kawamura E., Himmelspach R., Rashbrooke M. C., Whittington A. T., Gale K. R., Collings D. A., et al. (2006). MICROTUBULE ORGANIZATION 1 regulates structure and function of microtubule arrays during mitosis and cytokinesis in the Arabidopsis root. PubMed DOI PMC

Kner P., Chhun B. B., Griffis E. R., Winoto L., Gustafsson M. G. L. (2009). Super-resolution video microscopy of live cells by structured illumination. PubMed DOI PMC

Kollárová E., Baquero Forero A., Stillerová L., Přerostová S., Cvrčková F. (2020). Arabidopsis class II formins AtFH13 and AtFH14 can form heterodimers but exhibit distinct patterns of cellular localization. PubMed DOI PMC

Komis G., Illés P., Beck M., Šamaj J. (2011). Microtubules and mitogen-activated protein kinase signalling. PubMed DOI

Komis G., Mistrik M., Šamajová O., Doskočilová A., Ovečka M., Illés P., et al. (2014). Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy. PubMed DOI PMC

Komis G., Mistrik M., Šamajová O., Ovečka M., Bartek J., Šamaj J. (2015). Superresolution live imaging of plant cells using structured illumination microscopy. PubMed DOI

Komis G., Novák D., Ovečka M., Šamajová O., Šamaj J. (2018). Advances in imaging plant cell dynamics. PubMed DOI PMC

Korobchevskaya K., Lagerholm B. C., Colin-York H., Fritzsche M. (2017). Exploring the potential of airyscan microscopy for live cell imaging. DOI

Korolev A. V., Buschmann H., Doonan J. H., Lloyd C. W. (2007). AtMAP70-5, a divergent member of the MAP70 family of microtubule-associated proteins, is required for anisotropic cell growth in PubMed DOI

Korolev A. V., Chan J., Naldrett M. J., Doonan J. H., Lloyd C. W. (2005). Identification of a novel family of 70 kDa microtubule-associated proteins in Arabidopsis cells. PubMed DOI

Kosetsu K., Matsunaga S., Nakagami H., Colcombet J., Sasabe M., Soyano T., et al. (2010). The MAP kinase MPK4 is required for cytokinesis in PubMed DOI PMC

Krtková J., Benáková M., Schwarzerová K. (2016). Multifunctional microtubule-associated proteins in plants. PubMed DOI PMC

Lazzaro M. D., Wu S., Snouffer A., Wang Y., van der Knaap E. (2018). Plant organ shapes are regulated by protein interactions and associations with microtubules. PubMed DOI PMC

Ledbetter M. C., Porter K. R. (1963). A “microtubule” in plant cell fine structure. PubMed DOI PMC

Lee Y.-R. J., Liu B. (2013). The rise and fall of the phragmoplast microtubule array. PubMed DOI

Li H., Sun B., Sasabe M., Deng X., Machida Y., Lin H., et al. (2017). Arabidopsis MAP65-4 plays a role in phragmoplast microtubule organization and marks the cortical cell division site. PubMed DOI

Li H., Vaughan J. C. (2018). Switchable fluorophores for single-molecule localization microscopy. PubMed DOI PMC

Liesche J., Ziomkiewicz I., Schulz A. (2013). Super-resolution imaging with pontamine fast scarlet 4BS enables direct visualization of cellulose orientation and cell connection architecture in onion epidermis cells. PubMed DOI PMC

Lindeboom J. J., Nakamura M., Hibbel A., Shundyak K., Gutierrez R., Ketelaar T., et al. (2013). A mechanism for reorientation of cortical microtubule arrays driven by microtubule severing. PubMed DOI

Lindeboom J. J., Nakamura M., Saltini M., Hibbel A., Walia A., Ketelaar T., et al. (2019). CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation. PubMed DOI PMC

Lucas J. R., Courtney S., Hassfurder M., Dhingra S., Bryant A., Shaw S. L. (2011). Microtubule-associated proteins MAP65-1 and MAP65-2 positively regulate axial cell growth in etiolated PubMed DOI PMC

Lucas J. R., Shaw S. L. (2012). MAP65-1 and MAP65-2 promote cell proliferation and axial growth in Arabidopsis roots. PubMed DOI

Luptovčiak I., Komis G., Takáč T., Ovečka M., Šamaj J. (2017). Katanin: a sword cutting microtubules for cellular, developmental, and physiological purposes. PubMed DOI PMC

Ma Q., Sun J., Mao T. (2016). Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated PubMed DOI

Manders E. M. M., Stap J., Brakenhoff G. J., Van Driel R., Aten J. A. (1992). Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy. PubMed

Mao G. (2006). The role of MAP65-1 in microtubule bundling during Zinnia tracheary element formation. PubMed DOI

Marcus A., Raulet D. H. (2013). A simple and effective method for differentiating GFP and YFP by flow cytometry using the violet laser. PubMed DOI PMC

Molines A. T., Marion J., Chabout S., Besse L., Dompierre J. P., Mouille G., et al. (2018). EB1 contributes to microtubule bundling and organization, along with root growth, in PubMed DOI PMC

Müller S., Wright A. J., Smith L. G. (2009). Division plane control in plants: new players in the band. PubMed DOI

Nakagawa T., Suzuki T., Murata S., Nakamura S., Hino T., Maeo K., et al. (2007). Improved gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. PubMed DOI

Nakamura M. (2015). Microtubule nucleating and severing enzymes for modifying microtubule array organization and cell morphogenesis in response to environmental cues. PubMed DOI

Nakamura M., Ehrhardt D. W., Hashimoto T. (2010). Microtubule and katanin-dependent dynamics of microtubule nucleation complexes in the acentrosomal PubMed DOI

Nakamura M., Lindeboom J. J., Saltini M., Mulder B. M., Ehrhardt D. W. (2018). SPR2 protects minus ends to promote severing and reorientation of plant cortical microtubule arrays. PubMed DOI PMC

Oda Y. (2018). Emerging roles of cortical microtubule–membrane interactions. PubMed DOI

Panteris E., Galatis B. (2005). The morphogenesis of lobed plant cells in the mesophyll and epidermis: organization and distinct roles of cortical microtubules and actin filaments. PubMed DOI

Pastuglia M., Azimzadeh J., Goussot M., Camilleri C., Belcram K., Evrard J.-L., et al. (2006). γ-tubulin is essential for microtubule organization and development in PubMed DOI PMC

Pesquet E., Korolev A. V., Calder G., Lloyd C. W. (2010). The microtubule-associated protein AtMAP70-5 regulates secondary wall patterning in PubMed DOI

Sahl S. J., Hell S. W., Jakobs S. (2017). Fluorescence nanoscopy in cell biology. PubMed DOI

Sapala A., Runions A., Routier-Kierzkowska A.-L., Das Gupta M., Hong L., Hofhuis H., et al. (2018). Why plants make puzzle cells, and how their shape emerges. PubMed DOI PMC

Sasabe M., Kosetsu K., Hidaka M., Murase A., Machida Y. (2011). PubMed DOI PMC

Sassi M., Ali O., Boudon F., Cloarec G., Abad U., Cellier C., et al. (2014). An auxin-mediated shift toward growth isotropy promotes organ formation at the shoot meristem in Arabidopsis. PubMed DOI

Schneider C. A., Rasband W. S., Eliceiri K. W. (2012). NIH image to ImageJ: 25 years of image analysis. PubMed DOI PMC

Schneider R., Klooster K., van’t, Picard K., van der Gucht J., Demura T., Janson M., et al. (2020). Long-term single-cell imaging and simulations of microtubules reveal driving forces for wall pattering during proto-xylem development. PubMed DOI PMC

Schneider R., Persson S. (2015). Connecting two arrays: the emerging role of actin-microtubule cross-linking motor proteins. PubMed DOI PMC

Schubert V. (2017). Super-resolution microscopy – applications in plant cell research. PubMed DOI PMC

Schubert V., Weisshart K. (2015). Abundance and distribution of RNA polymerase II in PubMed DOI PMC

Sen T., Mamontova A. V., Titelmayer A. V., Shakhov A. M., Astafiev A. A., Acharya A., et al. (2019). Influence of the first chromophore-forming residue on photobleaching and oxidative photoconversion of EGFP and EYFP. PubMed DOI PMC

Shaw S. L., Kamyar R., Ehrhardt D. W. (2003). Sustained microtubule treadmilling in PubMed DOI

Shcherbakova D. M., Sengupta P., Lippincott-Schwartz J., Verkhusha V. V. (2014). Photocontrollable fluorescent proteins for superresolution imaging. PubMed DOI PMC

Smal I., Grigoriev I., Akhmanova A., Niessen W. J., Meijering E. (2010). Microtubule dynamics analysis using kymographs and variable-rate particle filters. PubMed DOI

Smertenko A. (2018). Phragmoplast expansion: the four-stroke engine that powers plant cytokinesis. PubMed DOI

Smertenko A. P., Chang H.-Y., Wagner V., Kaloriti D., Fenyk S., Sonobe S., et al. (2004). The Arabidopsis microtubule-associated protein AtMAP65-1: molecular analysis of its microtubule bundling activity. PubMed DOI PMC

Smertenko A. P., Cheng H.-Y., Sosobe S., Fenyk S. I., Weingartner M., Bögre L., et al. (2006). Control of the AtMAP65-1 interaction with microtubules through the cell cycle. PubMed DOI

Smertenko A. P., Kaloriti D., Chang H.-Y., Fiserova J., Opatrny Z., Hussey P. J. (2008). The C-terminal variable region specifies the dynamic properties of PubMed DOI PMC

Stoppin-Mellet V., Fache V., Portran D., Martiel J. L., Vantard M. (2013). MAP65 coordinate microtubule growth during bundle formation. PubMed DOI PMC

Subramanian R., Wilson-Kubalek E. M., Arthur C. P., Bick M. J., Campbell E. A., Darst S. A., et al. (2010). Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein. PubMed DOI PMC

Sun H., Furt F., Vidali L. (2018). Myosin XI localizes at the mitotic spindle and along the cell plate during plant cell division in PubMed DOI

Sun T., Li S., Ren H. (2017). OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice. PubMed DOI PMC

Tian J., Kong Z. (2019). The role of the augmin complex in establishing microtubule arrays. PubMed DOI

Tokunaga M., Imamoto N., Sakata-Sogawa K. (2008). Highly inclined thin illumination enables clear single-molecule imaging in cells. PubMed DOI

True J. H., Shaw S. L. (2020). Exogenous auxin induces transverse microtubule arrays through TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX receptors. PubMed DOI PMC

Tulin A., McClerklin S., Huang Y., Dixit R. (2012). Single-molecule analysis of the microtubule cross-linking protein MAP65-1 reveals a molecular mechanism for contact-angle-dependent microtubule bundling. PubMed DOI PMC

van Damme D., van Poucke K., Boutant E., Ritzenthaler C., Inzé D., Geelen D. (2004). In vivo dynamics and differential microtubule-binding activities of MAP65 proteins. PubMed DOI PMC

Vavrdová T., Šamaj J., Komis G. (2019a). Phosphorylation of plant microtubule-associated proteins during cell division. PubMed DOI PMC

Vavrdová T., Šamajová O., Křenek P., Ovečka M., Floková P., Šnaurová R., et al. (2019b). Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins. PubMed DOI PMC

Vineyard L., Elliott A., Dhingra S., Lucas J. R., Shaw S. L. (2013). Progressive transverse microtubule array organization in hormone-induced PubMed DOI PMC

Vizcay-Barrena G., Webb S. E. D., Martin-Fernandez M. L., Wilson Z. A. (2011). Subcellular and single-molecule imaging of plant fluorescent proteins using total internal reflection fluorescence microscopy (TIRFM). PubMed DOI PMC

Walia A., Nakamura M., Moss D., Kirik V., Hashimoto T., Ehrhardt D. W. (2014). GCP-WD mediates γ-TuRC recruitment and the geometry of microtubule nucleation in interphase arrays of PubMed DOI

Wang B., Liu G., Zhang J., Li Y., Yang H., Ren D. (2018). The RAF-like mitogen-activated protein kinase kinase kinases RAF22 and RAF28 are required for the regulation of embryogenesis in Arabidopsis. PubMed DOI

Werner S., Marillonnet S., Hause G., Klimyuk V., Gleba Y. (2006). Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A. PubMed DOI PMC

Wightman R., Turner S. R. (2007). Severing at sites of microtubule crossover contributes to microtubule alignment in cortical arrays. PubMed DOI

Wu S.-Z., Bezanilla M. (2014). Myosin VIII associates with microtubule ends and together with actin plays a role in guiding plant cell division. PubMed DOI PMC

Wu S.-Z., Bezanilla M. (2018). Actin and microtubule cross talk mediates persistent polarized growth. PubMed DOI PMC

Xu T., Qu Z., Yang X., Qin X., Xiong J., Wang Y., et al. (2009). A cotton kinesin GhKCH2 interacts with both microtubules and microfilaments. PubMed DOI

Yamada M., Goshima G. (2017). Mitotic spindle assembly in land plants: molecules and mechanisms. PubMed DOI PMC

Zhang M., Chang H., Zhang Y., Yu J., Wu L., Ji W., et al. (2012). Rational design of true monomeric and bright photoactivatable fluorescent proteins. PubMed DOI

Zhang Q., Fishel E., Bertroche T., Dixit R. (2013). Microtubule severing at crossover sites by katanin generates ordered cortical microtubule arrays in PubMed DOI

Zhu C., Ganguly A., Baskin T. I., McClosky D. D., Anderson C. T., Foster C., et al. (2015). The fragile Fiber1 kinesin contributes to cortical microtubule-mediated trafficking of cell wall components. PubMed DOI PMC

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