Enzyme-powered micro- and nanomotors are tiny devices inspired by nature that utilize enzyme-triggered chemical conversion to release energy stored in the chemical bonds of a substrate (fuel) to actuate it into active motion. Compared with conventional chemical micro-/nanomotors, these devices are particularly attractive because they self-propel by utilizing biocompatible fuels, such as glucose, urea, glycerides, and peptides. They have been designed with functional material constituents to efficiently perform tasks related to active targeting, drug delivery and release, biosensing, water remediation, and environmental monitoring. Because only a small number of enzymes have been exploited as bioengines to date, a new generation of multifunctional, enzyme-powered nanorobots will emerge in the near future to selectively search for and utilize water contaminants or disease-related metabolites as fuels. This Minireview highlights recent progress in enzyme-powered micro- and nanomachines.

Center for Advanced Functional Nanorobots Department of Inorganic Chemistry Faculty of Chemical Technology University of Chemistry and Technology Prague Technická 5 16628 Prague Czech Republic

Department of Chemical and Biomolecular Engineering Yonsei University 50 Yonsei ro Seodaemun gu Seoul 03722 Korea

Department of Medical Research China Medical University Hospital China Medical University No 91 Hsueh Shih Road Taichung 40402 Taiwan

Department of Polymers Faculty of Chemical Technology University of Chemistry and Technology Prague Technická 5 16628 Prague Czech Republic

Future Energy and Innovation Laboratory Central European Institute of Technology Brno University of Technology Purkyňova 656 123 Brno 616 00 Czech Republic

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R. Mallik, S. P. Gross, Curr. Biol. 2004, 14, R971-R982.

X. Ma, A. C. Hortelão, T. Patiño, S. Sánchez, ACS Nano 2016, 10, 9111-9122.

C. Riedel, R. Gabizon, C. A. M. Wilson, K. Hamadani, K. Tsekouras, S. Marqusee, S. Pressé, C. Bustamante, Nature 2015, 517, 227;

R. Golestanian, Phys. Rev. Lett. 2015, 115, 108102.

X. Zhao, K. Gentile, F. Mohajerani, A. Sen, Acc. Chem. Res. 2018, 51, 2373-2381.

A.-Y. Jee, S. Dutta, Y.-K. Cho, T. Tlusty, S. Granick, Proc. Natl. Acad. Sci. USA 2018, 115, 14-18.

S. Sengupta, K. K. Dey, H. S. Muddana, T. Tabouillot, M. E. Ibele, P. J. Butler, A. Sen, J. Am. Chem. Soc. 2013, 135, 1406-1414.

K. K. Dey, X. Zhao, B. M. Tansi, W. J. Méndez-Ortiz, U. M. Córdova-Figueroa, R. Golestanian, A. Sen, Nano Lett. 2015, 15, 8311-8315.

S. Sanchez, A. A. Solovev, Y. Mei, O. G. Schmidt, J. Am. Chem. Soc. 2010, 132.

J. Orozco, V. García-Gradilla, M. D'Agostino, W. Gao, A. Cortés, J. Wang, ACS Nano 2013, 7, 818-824.

W. Waldhäusl, G. Kleinberger, A. Korn, R. Dudczak, P. Bratusch-Marrain, P. Nowotny, Diabetes 1979, 28, 577-584.

M. Yamaguchi, M. Mitsumori, Y. Kano, Proceedings of the 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Vol. 20, Biomedical Engineering Towards the Year 2000 and Beyond (Cat. No.98CH36286) 1998, pp. 1763-1766.

N. Mano, A. Heller, J. Am. Chem. Soc. 2005, 127, 11574-11575.

D. Pantarotto, W. R. Browne, B. L. Feringa, Chem. Commun. 2008, 1533-1535.

J. Kopstein, O. M. Wrong, Clin. Sci. 1977, 52, 9-17.

L. Magos, J. Appl. Toxicol. 1987, 7, 413.

A. C. Hortelão, T. Patiño, A. Perez-Jiménez, À. Blanco, S. Sánchez, Adv. Funct. Mater. 2018, 28, 1705086.

T. Patino, A. Porchetta, A. Jannasch, A. Lladó, T. Stumpp, E. Schäffer, F. Ricci, S. Sánchez, Nano Lett. 2019, 19, 3440-3447.

S. Sattayasamitsathit, K. Kaufmann, M. Galarnyk, R. Vazquez-Duhalt, J. Wang, RSC Adv. 2014, 4, 27565-27570.

Z. Wu, X. Lin, X. Zou, J. Sun, Q. He, ACS Appl. Mater. Interfaces 2015, 7, 250-255.

V. V. Singh, K. Kaufmann, B. Esteban-Fernández de Ávila, M. Uygun, J. Wang, Chem. Commun. 2016, 52, 3360-3363.

X. Ma, A. C. Hortelao, A. Miguel-López, S. Sánchez, J. Am. Chem. Soc. 2016, 138, 13782-13785.

P. Pinyou, V. Blay, L. M. Muresan, T. Noguer, Mater. Horiz. 2019, 6, 1336-1358;

H. H. Nguyen, S. H. Lee, U. J. Lee, C. D. Fermin, M. Kim, Materials 2019, 12, 121;

S. Ferri, K. Kojima, K. Sode, J. Diab. Sci. Technol. 2011, 5, 1068-1076.

J. Kaushal, S. Mehandia, G. Singh, A. Raina, S. K. Arya, Biocatal. Agricult. Biotechnol. 2018, 16, 192-199.

A. N. P. Hiner, J. Hernández-Ruiz, G. A. Williams, M. B. Arnao, F. García-Cánovas, M. Acosta, Arch. Biochem. Biophys. 2001, 392, 295-302.

H. Y. Chung, B. S. Baek, S. H. Song, M. S. Kim, J. I. Huh, K. H. Shim, K. W. Kim, K. H. Lee, Age 1997, 20, 127-140;

E. E. Kelley, N. K. H. Khoo, N. J. Hundley, U. Z. Malik, B. A. Freeman, M. M. Tarpey, Free Rad. Biol. Med. 2010, 48, 493-498.

A. Commault, R. Weld, Whole-cell Biosensors for Monitoring Bioremediation in Biofilms in Bioremediation: Current Research and Emerging Technologies (Ed.: G. Lear). Caister Academic Press, Norfolk, 2016, pp. 75-92;

R. Ramesh, P. Selvan, J. Kumar, M. Gowthaman, S. D′Souza, N. Kamini, Mater. Sci. Engin. C 2015, 49, 786-792.

C. R. Carlini, R. Ligabue-Braun, Toxicon 2016, 110, 90-109.

M. Luo, S. Li, J. Wan, C. Yang, B. Chen, J. Guan, Langmuir 2020, 10.1021/acs.langmuir.9b03315.

L. Wang, A. C. Hortelão, X. Huang, S. Sánchez, Angew. Chem. Int. Ed. 2019, 58, 7992-7996;

Angew. Chem. 2019, 131, 8076-8080.

P. S. Schattling, M. A. Ramos-Docampo, V. Salgueiriño, B. Städler, ACS Nano 2017, 11, 3973-3983.

T. Patiño, N. Feiner-Gracia, X. Arqué, A. Miguel-López, A. Jannasch, T. Stumpp, E. Schäffer, L. Albertazzi, S. Sánchez, J. Am. Chem. Soc. 2018, 140, 7896-7903.

X. Ma, A. Jannasch, U.-R. Albrecht, K. Hahn, A. Miguel-López, E. Schäffer, S. Sánchez, Nano Lett. 2015, 15, 7043-7050.

X. Arqué, A. Romero-Rivera, F. Feixas, T. Patiño, S. Osuna, S. Sánchez, Nat. Commun. 2019, 10, 2826.

T. Patiño, X. Arqué, R. Mestre, L. Palacios, S. Sánchez, Acc. Chem. Res. 2018, 51, 2662-2671.

S. Gáspár, Nanoscale 2014, 6, 7757-7763;

L. Sonntag, J. Simmchen, V. Magdanz, Molecules 2019, 24, 3410.

R. Golestanian, Phys. Rev. Lett. 2009, 102, 188305.

I.-A. Pavel, A.-I. Bunea, S. David, S. Gáspár, ChemCatChem 2014, 6, 866-872.

K. K. Dey, A. Sen, J. Am. Chem. Soc. 2017, 139, 7666-7676;

F. Mohajerani, X. Zhao, A. Somasundar, D. Velegol, A. Sen, Biochemistry 2018, 57, 6256-6263.

A. S. Mikhailov, R. Kapral, Proc. Natl. Acad. Sci. USA 2015, 112, E3639-E3644.

A. Somasundar, S. Ghosh, F. Mohajerani, L. N. Massenburg, T. Yang, P. S. Cremer, D. Velegol, A. Sen, Nat. Nanotechnol. 2019, 14, 1129-1134.

J. Agudo-Canalejo, P. Illien, R. Golestanian, Nano Lett. 2018, 18, 2711-2717.

Y. Mei, G. Huang, A. A. Solovev, E. B. Ureña, I. Mönch, F. Ding, T. Reindl, R. K. Y. Fu, P. K. Chu, O. G. Schmidt, Adv. Mater. 2008, 20, 4085-4090;

A. A. Solovev, Y. Mei, E. Bermúdez Ureña, G. Huang, O. G. Schmidt, Small 2009, 5, 1688-1692;

Q. Chi, Z. Wang, F. Tian, J. A. You, S. Xu, Micromachines 2018, 9, 537.

P. Qiu, B. Ma, C.-T. Hung, W. Li, D. Zhao, Acc. Chem. Res. 2019, 52, 2928-2938.

Y. Zhou, G. Quan, Q. Wu, X. Zhang, B. Niu, B. Wu, Y. Huang, X. Pan, C. Wu, Acta Pharm. Sin. B 2018, 8, 165-177.

J. Simmchen, A. Baeza, D. Ruiz, M. J. Esplandiu, M. Vallet-Regí, Small 2012, 8, 2053-2059.

X. Ma, S. Sanchez, Chem. Commun. 2015, 51, 5467-5470.

X. Ma, X. Wang, K. Hahn, S. Sánchez, ACS Nano 2016, 10, 3597-3605;

X. Ma, S. Sánchez, Tetrahedron 2017, 73, 4883-4886.

P. Schattling, B. Thingholm, B. Städler, Chem. Mater. 2015, 27, 7412-7418.

L. Soler, C. Martínez-Cisneros, A. Swiersy, S. Sánchez, O. G. Schmidt, Lab Chip 2013, 13, 4299-4303;

V. Garcia-Gradilla, J. Orozco, S. Sattayasamitsathit, F. Soto, F. Kuralay, A. Pourazary, A. Katzenberg, W. Gao, Y. Shen, J. Wang, ACS Nano 2013, 7, 9232-9240.

A. C. Hortelão, R. Carrascosa, N. Murillo-Cremaes, T. Patiño, S. Sánchez, ACS Nano 2019, 13, 429-439.

J. N. Wilson, Y. Wang, J. J. Lavigne, U. H. F. Bunz, Chem. Commun. 2003, 1626-1627.

Y. Wu, X. Lin, Z. Wu, H. Möhwald, Q. He, ACS Appl. Mater. Interfaces 2014, 6, 10476-10481.

C. B. Goy, R. E. Chaile, R. E. Madrid, React. Funct. Polymers 2019, 145, 104314.

Y. Yu, J. Guo, M. Zou, L. Cai, Y. Zhao, Chem. Asian J. 2019, 14, 2417-2430.

S. Keller, S. P. Teora, G. X. Hu, M. Nijemeisland, D. A. Wilson, Angew. Chem. Int. Ed. 2018, 57, 9814-9817;

Angew. Chem. 2018, 130, 9962-9965.

A.-I. Bunea, I.-A. Pavel, S. David, S. Gáspár, Chem. Commun. 2013, 49, 8803-8805.

L. K. E. A. Abdelmohsen, M. Nijemeisland, G. M. Pawar, G.-J. A. Janssen, R. J. M. Nolte, J. C. M. van Hest, D. A. Wilson, ACS Nano 2016, 10, 2652-2660;

M. Nijemeisland, L. K. E. A. Abdelmohsen, W. T. S. Huck, D. A. Wilson, J. C. M. van Hest, ACS Cent. Sci. 2016, 2, 843-849.

D. A. Wilson, B. de Nijs, A. van Blaaderen, R. J. M. Nolte, J. C. M. van Hest, Nanoscale 2013, 5, 1315-1318.

Y. Men, W. Li, G.-J. Janssen, R. S. M. Rikken, D. A. Wilson, Nano Lett. 2018, 18, 2081-2085.

J. Sun, M. Mathesh, W. Li, D. A. Wilson, ACS Nano 2019, 13, 10191-10200.

B. J. Toebes, F. Cao, D. A. Wilson, Nat. Commun. 2019, 10, 5308.

M. Safaei, M. M. Foroughi, N. Ebrahimpoor, S. Jahani, A. Omidi, M. Khatami, Trends Anal. Chem. 2019, 118, 401-425.

S. Gao, J. Hou, J. Zeng, J. J. Richardson, Z. Gu, X. Gao, D. Li, M. Gao, D.-W. Wang, P. Chen, V. Chen, K. Liang, D. Zhao, B. Kong, Adv. Funct. Mater. 2019, 29, 1808900.

J. Wang, B. J. Toebes, A. S. Plachokova, Q. Liu, D. Deng, J. A. Jansen, F. Yang, D. A. Wilson, Adv. Healthcare Mater. 2020, 9, 1901710.