To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.

Center for Genome Engineering University of Minnesota Minneapolis Minnesota 55455 USA

Department of Genetics Cell Biology and Development University of Minnesota Minneapolis Minnesota 55455 USA

Department of Molecular Genetics Center for Applied Plant Sciences and Ohio State Biochemistry Program The Ohio State University Columbus Ohio 43210 USA

Department of Plant Developmental Genetics Institute of Biophysics ASCR v v i Kralovopolska street 135 Brno 612 00 Czech Republic

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Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants

Novel CRISPR-Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives

Wheat streak mosaic virus: a century old virus with rising importance worldwide