Seed description in morphology is often based on adjectives such as "spherical", "globular", or "reniform", but this does not provide a quantitative method. A new morphological approach based on the comparison of seed images with geometric models provides a seed description in Silene species on a quantitative basis. The novelty of the proposed method is based in the comparison of the seed images with geometric models according to a cardioid shape. The J index is a measurement that indicates the seed percentage of similarity with a cardioid or cardioid-derived figures used as models. The seeds of Silene species have high values of similarity with the cardioid and cardioid-derived models (J index superior to 90). The comparison with different figures allows species description and differentiation. The method is applied here to seeds of 21 species and models are proposed for some of them including S. diclinis, an endangered species. The method is discussed in the context of previous comparison with the measures used in traditional morphometric analysis. The similarity of seed images with geometric figures opens a new perspective for the automatized taxonomical evaluation of samples linking seed morphology to functional traits in endangered Silene species.

Biogeography Paleoecology and Nature Conservation Faculty of Biology and Environmental Protection University of Lodz 1 3 Banacha Str 90 237 Lodz Poland

IRNASA CSIC Cordel de Merinas 40 Salamanca Spain

Plant Developmental Genetics Institute of Biophysics v v i Academy of Sciences of the Czech Republic Královopolská 135 612 65 Brno Czech Republic

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Perveen A., Qaiser M. Pollen flora of Pakistan-LI- Caryophyllaceae. Pak. J. Bot. 2006;38:901–915.

Hernández-Ledesma P., Berendsohn W.G., Borsch T., Von Mering S., Akhani H., Arias S., Castañeda-Noa I., Eggli U., Eriksson R., Flores-Olvera H., et al. A taxonomic backbone for the global synthesis of species diversity in the angiosperm order Caryophyllales. Willdenowia. 2015;45:281. doi: 10.3372/wi.45.45301. DOI

Frajman B., Schönswetter P., Weiss-Schneeweiss H., Oxelman B. Origin and Diversification of South American Polyploid Silene Sect. Physolychnis (Caryophyllaceae) in the Andes and Patagonia. Front. Genet. 2018;9:639. doi: 10.3389/fgene.2018.00639. PubMed DOI PMC

Christenhusz M.J., Fay M.F., Chase M.W. Plants of the World: An Illustrated Encyclopedia of Vascular Plants. University of Chicago Press; Chicago, IL, USA: 2017.

Dadandi M.Y., Yildiz K. Seed morphology of some Silene L. (Caryophyllaceae) species collected from Turkey. Turk. J. Bot. 2015;39:280–297. doi: 10.3906/bot-1307-35. DOI

Fawzi N.M., Fawzy A.M., Mohamed A.A.-H.A. Seed Morphological Studies on Some Species of Silene L. (Caryophyllaceae) Int. J. Bot. 2010;6:287–292. doi: 10.3923/ijb.2010.287.292. DOI

Greuter W. Silene (Caryophyllaceae) in Greece: A subgeneric and sectional classification. TAXON. 1995;44:543–581. doi: 10.2307/1223499. DOI

Oxelman B., Rautenberg A., Thollesson M., Larsson A., Frajman B., Eggens F. Sileneae Taxonomy and Systematics. [(accessed on 27 May 2020)];2013 Available online: http://www.sileneae.info.

Ullah F., Ayaz A., Saqib S., Zaman W., Butt M.A., Ullah A. Silene conoidea L.: A Review on its Systematic, Ethnobotany and Phytochemical profile. Plant Sci. Today. 2019;6:373–382. doi: 10.14719/pst.2019.6.4.571. DOI

Mira S., González-Benito M.E., Ibars A.M., Estrelles E. Dormancy release and seed ageing in the endangered species Silene diclinis. Biodivers. Conserv. 2010;20:345–358. doi: 10.1007/s10531-010-9833-x. DOI

Alonso-Vargas M.Á., Guilló A., Pérez-Botella J., Crespo M.B., Juan A. Genetic assessment of population restorations of the critically endangered Silene hifacensis in the Iberian Peninsula. J. Nat. Conserv. 2014;22:532–538. doi: 10.1016/j.jnc.2014.08.007. DOI

Ferrer-Gallego P.P., Ferrando-Pardo I., Laguna E., Pérez-Rovira P., Jiménez J. Recovery plans of threatened flora of the Valencian Community. Results after three years since the approval of the legal normative. Flora Montiberica. 2019;73:48–61.

López-Pujol J., Font J., Simon J., Blanché C. Can the preservation of historical relicts permit the conservation of endangered plant species? The case of Silene sennenii (Caryophyllaceae) Conserv. Genet. 2006;8:903–912. doi: 10.1007/s10592-006-9245-3. DOI

Jafari F., Zarre S., Gholipour A., Eggens F., Rabeler R.K., Oxelman B. A new taxonomic backbone for the infrageneric classification of the species-rich genus Silene (Caryophyllaceae) TAXON. 2020;69:337–368. doi: 10.1002/tax.12230. DOI

Wofford B.E. External Seed Morphology of Arenaria (Caryophyllaceae) of the Southeastern United States. Syst. Bot. 1981;6:126. doi: 10.2307/2418543. DOI

Wyatt R. Intraspecific Variation in Seed Morphology of Arenaria uniflora (Caryophyllaceae) Syst. Bot. 1984;9:423. doi: 10.2307/2418791. DOI

Amini E., Zarre S., Assadi M. Seed micro-morphology and its systematic significance in Gypsophila (Caryophyllaceae) and allied genera. Nord. J. Bot. 2011;29:660–669. doi: 10.1111/j.1756-1051.2011.01208.x. DOI

Kovtonyuk N.K. The structure of seed Surface and the systematics of the Siberian Gypsophila species (Caryophyllaceae) Bot. Z. 1994;79:48–51.

Minuto L., Fior S., Roccotiello E., Casazza G. Seed morphology in Moehringia L. and its taxonomic significance in comparative studies within the Caryophyllaceae. Plant Syst. Evol. 2006;262:189–208. doi: 10.1007/s00606-006-0458-1. DOI

Kaplan A., Çölgeçen H., Büyükkartal H.N. Seed morphology and histology of some Paronychia taxa (Caryophyllaceae) from Turkey. Bangladesh J. Bot. 2009;38:171–176. doi: 10.3329/bjb.v38i2.5142. DOI

Crow G.E. The Systematic Significance of Seed Morphology in Sagina (Caryophyllaceae) Under Scanning Electron Microscopy. Brittonia. 1979;31:52–63. doi: 10.2307/2806673. DOI

Volponi C.R. Stellaria cuspidata (Caryophyllaceae) and some related species in the Andes. Willdenowia. 1993;23:193–209.

Poyraz I.E., Ataşlar E. Pollen and seed morphology of Velezia L. (Caryophyllaceae) genus in Turkey. Turk. J. Bot. 2010;34:179–190.

Hong S.-P., Han M.-J., Kim K.-J. Systematic significance of seed coat morphology in Silene L. s. str. (Sileneae-Caryophyllaceae) from Korea. J. Plant Biol. 1999;42:146–150. doi: 10.1007/BF03031023. DOI

Rohrbach P. Monographic der Gattung Silene. Verlag von Engelmann; Leipzig, Germany: 1869. pp. 1–249.

Chowdhuri P.K. Studies in the genus Silene. Notes. R. Bot. Gard. 1957;22:221–287.

Ghazanfar S.A. Seed characters as diagnostic in the perennial sections of the genus Silene (Family Caryophyllaceae) Pak. J. Bot. 1983;15:7–12.

Melzheimer V. On the taxonomic position of Silene thebana (Caryophyllaceae) Plant Syst. Evol. 1987;155:251–256. doi: 10.1007/BF00936302. DOI

Bojňanský V., Fargašová A. Atlas of Seeds and Fruits of Central and East-European Flora: The Carpathian Mountains Region. Springer; Berlin/Heidelberg, Germany: 2007. pp. 1–954.

Yildiz K., Cirpici A. Seed morphological studies of Silene L. from Turkey. J. Bot. 1998;30:173–188.

Hoseini E., Ghahremaninejad F., Assadi M., Edalatiyan M.N. Seed micromorphology and its implication in subgeneric classification of Silene (Caryophyllaceae, Sileneae) Flora Morphol. Distrib. Funct. Ecol. Plants. 2017;228:31–38. doi: 10.1016/j.flora.2017.01.006. DOI

Cervantes E., Gómez J.J.M. Seed Shape Description and Quantification by Comparison with Geometric Models. Horticulturae. 2019;5:60. doi: 10.3390/horticulturae5030060. DOI

Cervantes E., Martín J.J., Saadaoui E. Updated Methods for Seed Shape Analysis. Science. 2016;2016:1–10. doi: 10.1155/2016/5691825. PubMed DOI PMC

Cervantes E., Martín J.J., Ardanuy R., De Diego J.G., Tocino Á. Modeling the Arabidopsis seed shape by a cardioid: Efficacy of the adjustment with a scale change with factor equal to the Golden Ratio and analysis of seed shape in ethylene mutants. J. Plant Physiol. 2010;167:408–410. doi: 10.1016/j.jplph.2009.09.013. PubMed DOI

Cervantes E., Martín J.J., Chan P.K., Gresshoff P.M., Tocino Á. Seed shape in model legumes: Approximation by a cardioid reveals differences in ethylene insensitive mutants of Lotus japonicus and Medicago truncatula. J. Plant Physiol. 2012;169:1359–1365. doi: 10.1016/j.jplph.2012.05.019. PubMed DOI

Cervantes E., Saadaoui E., Tocino Á., Martín-Gómez J.J. The Model Legume Medicago truncatula. Wiley; Hoboken, NJ, USA: 2020. Seed Shape Quantification in the Model Legumes: Methods and Applications; pp. 92–98.

Saadaoui E., Martín J.J., Cervantes E. Seed morphology in Tunisian wild populations of Capparis spinosa L. Acta Biol. Cracov Ser. Bot. 2013;55:99–106. doi: 10.5897/AJB10.1429. DOI

Martín-Gómez J.J., Rewicz A., Cervantes E. Seed Shape Diversity in families of the Order Ranunculales. Phytotaxa. 2019;425:193–207. doi: 10.11646/phytotaxa.425.4.1. DOI

Gómez J.J.M., Del Pozo D.G., Cervantes E. Seed Shape Quantification in the Malvaceae Reveals Cardioid-Shaped Seeds Predominantly in Herbs. Botanica. 2019;25:21–31. doi: 10.2478/botlit-2019-0003. DOI

Cervantes E., Martín-Gómez J.J. Seed shape quantification in the order Cucurbitales. Mod. Phytomorphol. 2018;12:1–13. doi: 10.5281/zenodo.117487. DOI

Saadaoui E., Martín J.J., Bouazizi R., Ben Romdhane C., Grira M., Abdelkabir S., Khouja M.L., Cervantes E. Phenotypic variability and seed yield of Jatropha curcas L. introduced to Tunisia. Acta Bot. Mex. 2015;1:119. doi: 10.21829/abm110.2015.193. DOI

Gómez J.J.M., Saadaoui E., Cervantes E. Seed Shape of Castor Bean (Ricinus communis L.) Grown in Different Regions of Tunisia. J. Agric. Ecol. Res. Int. 2016;8:1–11. doi: 10.9734/JAERI/2016/23934. DOI

Gómez J.J.M., Rewicz A., Goriewa-Duba K., Wiwart M., Tocino A., Cervantes E., Gómez M., Duba G. Morphological Description and Classification of Wheat Kernels Based on Geometric Models. Agronomy. 2019;9:399. doi: 10.3390/agronomy9070399. DOI

Gómez J.J.M., Del Pozo D.G., Ucchesu M., Bacchetta G., De Santamaría F.C.S., Tocino A., Cervantes E. Seed Morphology in the Vitaceae Based on Geometric Models. Agronomy. 2020;10:739. doi: 10.3390/agronomy10050739. DOI

Del Pozo D.G., Gómez J.J.M., Tocino A., Cervantes E. Seed Geometry in the Arecaceae. Horticulturae. 2020;6:64. doi: 10.3390/horticulturae6040064. DOI

Brazeau M.D., Guillerme T., Smith M.R. An algorithm for Morphological Phylogenetic Analysis with Inapplicable Data. Syst. Biol. 2019;68:619–631. doi: 10.1093/sysbio/syy083. PubMed DOI PMC

Lee M.S.Y., Palci A. Morphological Phylogenetics in the Genomic Age. Curr. Biol. 2015;25:R922–R929. doi: 10.1016/j.cub.2015.07.009. PubMed DOI

Sukhorukov A.P., Nilova M.V., Krinitsina A.A., Zaika M.A., Erst A.S., Shepherd K.A. Molecular phylogenetic data and seed coat anatomy resolve the generic position of some critical Chenopodioideae (Chenopodiaceae—Amaranthaceae) with reduced perianth segments. PhytoKeys. 2018;109:103–128. doi: 10.3897/phytokeys.109.28956. PubMed DOI PMC

Slancarova V., Zdanska J., Janousek B., Talianova M., Zschach C., Zluvova J., Široký J., Kovacova V., Blavet H., Danihelka J., et al. Evolution of Sex Determination Systems with Heterogametic Males and Females In Silene. Evolution. 2013;67:3669–3677. doi: 10.1111/evo.12223. PubMed DOI

Bernasconi G., Antonovics J., Biere A.L., Charlesworth D., Delph L.F., Filatov D., Giraud T., Hood M., Marais G.A.B., McCauley D.W., et al. Silene as a model system in ecology and evolution. Heredity. 2009;103:5–14. doi: 10.1038/hdy.2009.34. PubMed DOI

Hill S.R. Conservation Assessment for Ovate Catchfly (Silene ovata) Pursh. USDA Forest Service, Eastern Region. [(accessed on 12 September 2020)]; Available online: https://core.ac.uk/reader/4819420.

Zumajo-Cardona C., Ambrose B.A. Phylogenetic analyses of key developmental genes provide insight into the complex evolution of seeds. Mol. Phylogenetics Evol. 2020;147:106778. doi: 10.1016/j.ympev.2020.106778. PubMed DOI

Ghimire B., Suh Y., Soltis U.E., Heo K. Phylogenetic relationships of Coreanomecon (Papaveraceae: Chelidonioideae) inferred from seed morphology and nrITS sequence data. Nord. J. Bot. 2019;37:211–225. doi: 10.1111/njb.02275. DOI

Stull G.W., Johnson D.M., Murray N.A., Couvreur T.L.P., Reeger J.E., Roy C.M. Plastid and Seed Morphology Data Support a Revised Infrageneric Classification and an African Origin of the Pantropical Genus Xylopia (Annonaceae) Syst. Bot. 2017;42:211–225. doi: 10.1600/036364417X695484. DOI

Bocquet G. Revisio Physolychnidum (Silene sect. Physolychnis). Phanerogamarum Monographiae 1. Lehre J. Cramer; Berlin, Germany: 1969.

eFloras. [(accessed on 24 November 2020)];2008 Available online: http://www.efloras.org.

Buide M.L. Pollination Ecology of Silene acutifolia (Caryophyllaceae): Floral Traits Variation and Pollinator Attraction. Ann. Bot. 2005;97:289–297. doi: 10.1093/aob/mcj032. PubMed DOI PMC

Eflorasys. [(accessed on 7 September 2020)]; Available online: http://eflorasys.univ-lorraine.fr/index.php/en/species/view/123446.

Silene conica. [(accessed on 15 September 2020)]; Available online: https://en.wikipedia.org/wiki/Silene_conica.

Montesinos D., Güemes J. Silenediclinis. The IUCN Red List of Threatened Species 2006. [(accessed on 7 September 2020)]; doi: 10.2305/IUCN.UK.2006.RLTS.T61640A12531397.en. Available online: DOI

Datenbank Biologisch-Ökologischer Merkmale der Flora von Deutschland. [(accessed on 7 September 2020)]; Available online: https://www.ufz.de/biolflor/

Anthos: Sistema de Información Sobre las Plantas de España. [(accessed on 7 September 2020)]; Available online: http://www.anthos.es/

Stroh P.A. Silene Otites (L.) Wibel. Spanish Catchfly. Species Account. Botanical Society of Britain and Ireland; Durham, UK: 2015. [(accessed on 7 September 2020)]. Available online: https://bsbi.org/wp-content/uploads/dlm_uploads/Silene_otites_species_account.pdf.

Silene uniflora. [(accessed on 15 September 2020)]; Available online: https://en.wikipedia.org/wiki/Silene_uniflora.

Floraweb The Federal Agency for Nature Conservation. Das Bundesamt für Naturschutz. [(accessed on 7 September 2020)];2020 Available online: http://www.floraweb.de/

Plants of the World Online. [(accessed on 7 September 2020)]; Available online: http://www.plantsoftheworldonline.org/

Sileneae Classification. [(accessed on 26 September 2020)]; Available online: http://www.sileneae.info/classification.php.

Ferreira T., Rasband W. ImageJ User Guide-IJ1.46r. [(accessed on 7 September 2020)];2012 Available online: http://imagej.nih.gov/ij/docs/guide.

Zdilla M.J., Hatfield S.A., McLean K.A., Cyrus L.M., Laslo J.M., Lambert H.W. Circularity, Solidity, Axes of a Best Fit Ellipse, Aspect Ratio, and Roundness of the Foramen Ovale. J. Craniofac. Surg. 2016;27:222–228. doi: 10.1097/SCS.0000000000002285. PubMed DOI PMC

Weisstein E.W. Cardioid. From MathWorld—A Wolfram Web Resource. [(accessed on 7 September 2020)]; Available online: https://mathworld.wolfram.com/Cardioid.html.

R Development Core Team . R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing; Vienna, Austria: 2019.

Box G.E.P., Cox D.R. An Analysis of Transformations. J. R. Stat. Soc. Ser. B Stat. Methodol. 1964;26:211–243. doi: 10.1111/j.2517-6161.1964.tb00553.x. DOI

Seed shape and size of Silene latifolia, differences between sexes, and influence of the parental genome in hybrids with Silene dioica

Quantitative Analysis of Seed Surface Tubercles in Silene Species

Curvature Analysis of Seed Silhouettes in Silene L

The Outline of Seed Silhouettes: A Morphological Approach to Silene (Caryophyllaceae)

New Geometric Models for Shape Quantification of the Dorsal View in Seeds of Silene Species