Rapid analysis of phenyl isothiocyanate derivatives of amino acids present in Czech meads
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
33848765
DOI
10.1016/j.chroma.2021.462134
PII: S0021-9673(21)00258-2
Knihovny.cz E-zdroje
- Klíčová slova
- Amino acids, Derivatization, HPLC, Mead, Phenyl isothiocyanate (PITC),
- MeSH
- alkoholické nápoje analýza MeSH
- aminokyseliny analýza MeSH
- chromatografie s reverzní fází MeSH
- isothiokyanatany analýza MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- aminokyseliny MeSH
- isothiokyanatany MeSH
- phenylisothiocyanate MeSH Prohlížeč
Amino acids (AAs) are minor compounds occurring in meads contributing to their final organoleptic properties. Determination of AAs profile and content can help to assess the mead authenticity, adulteration and thus its quality. This work deals with the optimization of rapid analysis of 21 AAs present in mead using reversed-phase high performance liquid chromatography with spectrophotometric detection after simple derivatization procedure with phenyl isothiocyanate agent without any sample pre-treatment. Optimized derivatization and separation conditions have been successfully applied to the quantification of AAs present in five Czech meads using the multiple point standard addition method. The total amino acid content was in the range of 134-828 mg/L. The content of proline was confirmed by Harmonised spectrophotometric method. Both chromatographic and spectrophotometric methods provided overlapping results in the range of 30-266 mg/L.
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