SARS-CoV-2 RNA may rarely be present in a uterine cervix LBC sample at the asymptomatic early stage of COVID 19 disease

. 2021 Nov ; 32 (6) : 766-770. [epub] 20210531

Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid34033165

Grantová podpora
FNPl 00669806 Ministry of Health of the Czech Republic

OBJECTIVE: Currently, it is thought that uterine cervix mucosal samples present a low risk of SARS-CoV-2 exposure. So far, there is no evidence of SARS-CoV-2 detection in Papanicolaou (Pap) smears. Nevertheless, clinicians could be exposed unaware to the coronavirus while performing and handling a Pap smear. We aimed to retrospectively evaluate the presence of SARS-CoV-2 RNA in cervical liquid-based cytology (LBC) samples in women who tested positive for a nasopharyngeal COVID-19 PCR test. METHODS: From our laboratory database, we identified patients with data on a cervical cancer screening LBC sample paired with a positive nasopharyngeal COVID-19 PCR test. Relevant LBC samples taken within an incubation period of 14 days and post-onset RNA shedding interval of 25 days were subsequently tested for SARS-CoV-2 RNA using RT-PCR tests. RESULTS: The study group consisted of 102 women. Of those, 23 LBC samples were tested. SARS-CoV-2 RNA was detected in one LBC sample from a 26-year-old asymptomatic woman taken six days before reporting headaches and knee arthralgia with a positive nasopharyngeal SARS-CoV-2 RT-PCR test. CONCLUSIONS: It is possible to detect SARS-CoV-2 RNA in cervical LBC samples at an early asymptomatic stage of COVID-19. In general, this finding is infrequent in asymptomatic women who tested SARS-CoV-2 positive within an incubation of 14 days and a post-onset RNA shedding period of 25 days. We fully support the current thinking that cervical LBC samples from asymptomatic women pose a low risk of SARS-CoV-2 exposure and can be handled in the frame of good microbiological practice and procedures.

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Vigliar E, Cepurnaite R, Alcaraz‐Mateos E, et al. Global impact of the COVID‐19 pandemic on cytopathology practice: results from an international survey of laboratories in 23 countries. Cancer Cytopathol. 2020;128:885‐894. PubMed

Wang YH, Bychkov A, Chakrabarti I, et al. Impact of the COVID‐19 pandemic on cytology practice: an international survey in the Asia‐Pacific region. Cancer Cytopathol. 2020;128:895‐904. PubMed

Virk RK, Wood T, Tiscornia‐Wasserman PG. Impact of COVID‐19 pandemic on functioning of cytopathology laboratory: Experience and perspective from an academic centre in New York. Cytopathology. 2021;32(3):304‐311. PubMed PMC

World Health Organisation (WHO) . Laboratory testing for coronavirus disease 2019 (COVID‐19) in suspected human cases. https://www.who.int/publications‐detail/laboratory‐testing‐for‐2019‐novel‐coronavirus‐in‐suspected‐humancases‐20200117. Accessed February 10, 2021

European Centre for Disease Prevention and Control . Infection prevention and control and preparedness for COVID‐19 in healthcare settings ‐ Sixth update. February 9, 2021. ECDC: Stockholm; 2021.

CEN Workshop AGREEMENT . CWA 15793 ‐ Laboratory biorisk management. https://www.aimst.edu.my/ibc/pdf/Guidelines/10.%20CWA%2015793,%202011.pdf. Accessed February 10, 2021

Ronchi A, Pagliuca F, Zito Marino F, Montella M, Franco R, Cozzolino I. Interventional cytopathology in the COVID‐19 era. Cytopathology. 2020;31:509‐513. PubMed PMC

Chen CC, Chi CY. Biosafety in the preparation and processing of cytology specimens with potential coronavirus (COVID‐19) infection: perspectives from Taiwan. Cancer Cytopathol. 2020;128:309‐316. PubMed PMC

Rossi ED, Fadda G, Mule A, Zannoni GF, Rindi G. Cytologic and histologic samples from patients infected by the novel coronavirus 2019 SARS‐CoV‐2: An Italian institutional experience focusing on biosafety procedures. Cancer Cytopathol. 2020;128:317‐320. PubMed PMC

Iaccarino A, Pisapia P, Vigliar E, Vielh P, Troncone G. Juggling the COVID‐19 pandemic: a cytopathology point of view. Cytopathology. 2021;32(3):299‐303. PubMed

Backer JA, Klinkenberg D, Wallinga J. Incubation period of 2019 novel coronavirus (2019‐nCoV) infections among travellers from Wuhan, China, 20–28 January 2020. Euro Surveill. 2020;25(5):2000062. PubMed PMC

Liu Y, Yan LM, Wan L, et al. Viral dynamics in mild and severe cases of COVID‐19. Lancet Infect Dis. 2020;20:656‐657. PubMed PMC

Lescure FX, Bouadma L, Nguyen D, et al. Clinical and virological data of the first cases of COVID‐19 in Europe: a case series. Lancet Infect Dis. 2020;20:697‐706. PubMed PMC

Carsana L, Sonzogni A, Nasr A, et al. Pulmonary post‐mortem findings in a series of COVID‐19 cases from northern Italy: a two‐centre descriptive study. Lancet Infect Dis. 2020;20:1135‐1140. PubMed PMC

Schurink B, Roos E, Radonic T, et al. Viral presence and immunopathology in patients with lethal COVID‐19: a prospective autopsy cohort study. Lancet Microbe. 2020;1:e290‐e299. PubMed PMC

Calabrese F, Pezzuto F, Fortarezza F, et al. Pulmonary pathology and COVID‐19: lessons from autopsy. The experience of European Pulmonary Pathologists. Virchows Arch. 2020;477:359‐372. PubMed PMC

Skok K, Stelzl E, Trauner M, et al. Post‐mortem viral dynamics and tropism in COVID‐19 patients in correlation with organ damage. Virchows Arch. 2021;478(2):343‐353. PubMed PMC

Coccolini F, Tartaglia D, Puglisi A, et al. SARS‐CoV‐2 is present in peritoneal fluid in COVID‐19 patients. Ann Surg. 2020;272:e240‐e242. PubMed PMC

Lersy F, Benotmane I, Helms J et al. Cerebrospinal fluid features in patients with coronavirus disease 2019 and neurologic manifestations: correlation with brain magnetic resonance imaging findings in 58 patients. J Infect Dis. 2021;223:600‐609. PubMed PMC

Gonzalez DC, Khodamoradi K, Pai R, et al. A systematic review on the investigation of SARS‐CoV‐2 in semen. Res Rep Urol. 2020;12:615‐621. PubMed PMC

Bertino E, Moro GE, De Renzi G, et al. Detection of SARS‐CoV‐2 in milk from COVID‐19 positive mothers and follow‐up of their infants. Front Pediatr. 2020;8:597699. PubMed PMC

Gagneur A, Dirson E, Audebert S, et al. Materno‐fetal transmission of human coronaviruses: a prospective pilot study. Eur J Clin Microbiol Infect Dis. 2008;27:863‐866. PubMed PMC

Joynt GM, Wu WK. Understanding COVID‐19: what does viral RNA load really mean? Lancet Infect Dis. 2020;20:635‐636. PubMed PMC

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