• MeSH
• antivirové látky aplikace a dávkování   terapeutické užití   MeSH
• COVID-19 * diagnóza   terapie   MeSH
• hospitalizace * MeSH
• lidé MeSH
• rizikové faktory MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• směrnice pro lékařskou praxi MeSH

• MeSH
• ambulantní péče * MeSH
• antivirové látky aplikace a dávkování   terapeutické užití   MeSH
• COVID-19 * diagnóza   terapie   MeSH
• lidé MeSH
• rizikové faktory MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• směrnice pro lékařskou praxi MeSH

Coronavirus Anxiety Scale (CAS) is a widely used measure that captures somatic symptoms of coronavirus-related anxiety. In a large-scale collaboration spanning 60 countries (Ntotal = 21,513), we examined the CAS's measurement invariance and assessed the convergent validity of CAS scores in relation to the fear of COVID-19 (FCV-19S) and the satisfaction with life (SWLS-3) scales. We utilized both conventional exact invariance tests and alignment procedures, with results revealing that the single-factor model fit the data well in almost all countries. Partial scalar invariance was supported in a subset of 56 countries. To ensure the robustness of results, given the unbalanced samples, we employed resampling techniques both with and without replacement and found the results were more stable in larger samples. The alignment procedure demonstrated a high degree of measurement invariance with 9% of the parameters exhibiting noninvariance. We also conducted simulations of alignment using the parameters estimated in the current model. Findings demonstrated reliability of the means but indicated challenges in estimating the latent variances. Strong positive correlations between CAS and FCV-19S estimated with all three different approaches were found in most countries. Correlations of CAS and SWLS-3 were weak and negative but significantly differed from zero in several countries. Overall, the study provided support for the measurement invariance of the CAS and offered evidence of its convergent validity while also highlighting issues with variance estimation. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

• MeSH
• COVID-19 * diagnóza   MeSH
• lidé MeSH
• psychometrie metody   MeSH
• reprodukovatelnost výsledků MeSH
• strach MeSH
• úzkost * diagnóza   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: Coronavirus disease 2019 (COVID-19) is a life-threatening disease with a heterogeneous course. Even some young patients are at increased risk of severe course or death, as they can face severe complications. It would be very useful to have a cheap and easily available marker to predict COVID-19 course in the early stages of the disease. The COVID-19 prognostic score could be a very useful clinical indicator available at the time of primary contact with the patient. METHODS: The COVID-19 prognostic score and the clinical condition together with selected laboratory parameters were evaluated in patients with respiratory tract infection and a positive PCR test for the SARS-CoV-2 during the first contact with the patient. Prognostic significance was evaluated using receiver operating characteristic curves (ROC) and area under the curve (AUC). Selected parameters of the blood count and hemostasis, as well as selected biochemical indicators, were examined too. RESULTS: Thirty-seven of 164 patients developed serious symptoms. The COVID-19 score had one of the highest AUC values (0.855) of all markers. The highest combination of sensitivity (91.9%) and specificity (71.7%) for identifying patients with a subsequent moderate and severe course of the disease was achieved at the threshold 1.5. The predictive value of a negative test is beneficial too (0.968). CONCLUSIONS: The COVID-19 prognostic score is a promising indicator stratifying patients with COVID-19 into prognostic groups at the time of the first contact, thus allowing the timely provision of increased care in patients at high risk of severe development.

• MeSH
• COVID-19 * diagnóza   MeSH
• infekce dýchací soustavy * MeSH
• lidé MeSH
• prognóza MeSH
• retrospektivní studie MeSH
• ROC křivka MeSH
• SARS-CoV-2 MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

This study presents a graphene field-effect transistor (gFET) biosensor with dual detection capabilities for SARS-CoV-2: one RNA detection assay to confirm viral positivity and the other for nucleocapsid (N-)protein detection as a proxy for infectiousness of the patient. This technology can be rapidly adapted to emerging infectious diseases, making an essential tool to contain future pandemics. To detect viral RNA, the highly conserved E-gene of the virus was targeted, allowing for the determination of SARS-CoV-2 presence or absence using nasopharyngeal swab samples. For N-protein detection, specific antibodies were used. Tested on 213 clinical nasopharyngeal samples, the gFET biosensor showed good correlation with RT-PCR cycle threshold values, proving its high sensitivity in detecting SARS-CoV-2 RNA. Specificity was confirmed using 21 pre-pandemic samples positive for other respiratory viruses. The gFET biosensor had a limit of detection (LOD) for N-protein of 0.9 pM, establishing a foundation for the development of a sensitive tool for monitoring active viral infection. Results of gFET based N-protein detection corresponded to the results of virus culture in all 16 available clinical samples and thus it also proved its capability to serve as a proxy for infectivity. Overall, these findings support the potential of the gFET biosensor as a point-of-care device for rapid diagnosis of SARS-CoV-2 infection and indirect assessment of infectiousness in patients, providing additional information for clinical and public health decision-making.

• MeSH
• biosenzitivní techniky * přístrojové vybavení   metody   MeSH
• COVID-19 * diagnóza   virologie   MeSH
• design vybavení MeSH
• elektronické tranzistory MeSH
• fosfoproteiny MeSH
• grafit * chemie   MeSH
• koronavirové nukleokapsidové proteiny izolace a purifikace   MeSH
• lidé MeSH
• limita detekce MeSH
• nazofarynx virologie   MeSH
• RNA virová * izolace a purifikace   analýza   MeSH
• SARS-CoV-2 * izolace a purifikace   genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Cílem tohoto článku je podat praktické informace o onemocnění covidem-19 se zaměřením na jeho kritéria nutná k uznání jako nemoci z povolání, na vývoj počtu případů s touto diagnózou uznaných jako nemoc z povolání v České republice mezi roky 2020–2022 a na definici reinfekce se shrnutím podmínek pro potvrzení její diagnózy při posuzování opakovaného onemocnění. V článku je stručně zrekapitulován průběh pandemie koronaviru na území České republiky včetně vývoje situace, počtu nově potvrzených případů nakažených a případů reinfekcí až do současnosti. Dále článek popisuje kazuistiku pacienta, u kterého se šetřilo opakované onemocnění covidem-19 jako nemoc z povolání. Klinické i hygienické podmínky pro uznání obou prodělaných onemocnění sice splněny byly, avšak kritéria pro uznání druhého onemocnění jako reinfekci splněny nebyly a nebylo tedy možné uznat onemocnění jako dvě samostatné nemoci z povolání.

The aim of this article is to provide practical information about the disease COVID-19 with a focus on its criteria necessary for recognition as an occupational disease, on the development of the number of cases with this diagnosis recognized as an occupational disease in the Czech Republic between the years 2020 and 2022 and on the definition of reinfection with a summary of the conditions for confirming its diagnosis when assessing recurrent disease. The article briefly recapitulates the course of the coronavirus pandemic in the Czech Republic, including the development of the situation, the number of newly confirmed cases of infection and cases of reinfection up to the present. Furthermore, the article describes a case study of a patient who was treated for recurrent disease of COVID-19 as a matter of professionalism. Although the clinical and hygienic conditions for recognition of both acquired diseases were met, the criteria for recognition of the second disease as reinfection were not met and it was therefore not possible to recognize the disease as two separate occupational diseases.

• MeSH
• COVID-19 * diagnóza   MeSH
• dospělí MeSH
• lidé MeSH
• nemoci z povolání * diagnóza   epidemiologie   MeSH
• pandemie MeSH
• posuzování pracovní neschopnosti MeSH
• reinfekce diagnóza   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

PURPOSE: Incident delirium is a frequent complication among hospitalized older people with COVID-19, associated with increased length of hospital stay, higher morbidity and mortality rates. Although delirium is preventable with early detection, systematic assessment methods and predictive models are not universally defined, thus delirium is often underrated. In this study, we tested the role of the Multidimensional Prognostic Index (MPI), a prognostic tool based on Comprehensive Geriatric Assessment, to predict the risk of incident delirium. METHODS: Hospitalized older patients (≥ 65 years) with COVID-19 infection were enrolled (n = 502) from ten centers across Europe. At hospital admission, the MPI was administered to all the patients and two already validated delirium prediction models were computed (AWOL delirium risk-stratification score and Martinez model). Delirium occurrence during hospitalization was ascertained using the 4A's Test (4AT). Accuracy of the MPI and the other delirium predictive models was assessed through logistic regression models and the area under the curve (AUC). RESULTS: We analyzed 293 patients without delirium at hospital admission. Of them 33 (11.3%) developed delirium during hospitalization. Higher MPI score at admission (higher multidimensional frailty) was associated with higher risk of incident delirium also adjusting for the other delirium predictive models and COVID-19 severity (OR = 12.72, 95% CI = 2.11-76.86 for MPI-2 vs MPI-1, and OR = 33.44, 95% CI = 4.55-146.61 for MPI-3 vs MPI-1). The MPI showed good accuracy in predicting incident delirium (AUC = 0.71) also superior to AWOL tool, (AUC = 0.63) and Martinez model (AUC = 0.61) (p < 0.0001 for both comparisons). CONCLUSIONS: The MPI is a sensitive tool for early identification of older patients with incident delirium.

• MeSH
• COVID-19 * komplikace   epidemiologie   diagnóza   MeSH
• delirium * diagnóza   epidemiologie   MeSH
• geriatrické hodnocení * metody   MeSH
• hodnocení rizik MeSH
• hospitalizace * statistika a číselné údaje   MeSH
• incidence MeSH
• lidé MeSH
• prognóza MeSH
• prospektivní studie MeSH
• rizikové faktory MeSH
• SARS-CoV-2 MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• multicentrická studie MeSH
• Geografické názvy
• Evropa MeSH

Počet diagnostikovaných a hlášených případů infekce virem SARS-CoV-2 vyvolávajícího onemocnění covid-19, které přerostlo v celosvětovou pandemii, zůstává zatím od května roku 2023, kdy Světová zdravotnická organizace vyhlásila její ukončení, ve všech zemích včetně České republiky trvale nízký. Je ale nutno říci, že opatření realizovaná v boji s touto infekcí nesplnila všechna očekávání. Přestože stále vznikají nové mutace viru, které mohou potenciálně vyvolávat onemocnění, ukazuje se, že většina lidí se s nimi naučila postupně spoluexistovat. Vzhledem k některým unikátním vlastnostem viru SARS-CoV-2 a jeho variant však budou stále existovat predisponovaní jedinci, kteří onemocní a bude nutné poskytnout jim jak hospitalizaci, tak i efektivní léčbu podpořenou a monitorovanou adekvátními laboratorními vyšetřeními. Tento článek je komentářem k této problematice a zabývá se především diagnostikou a péčí o jedince postižené tímto infekčním onemocněním v jeho časné fázi. Autorský překlad článku do anglické verze je dostupný na: https://www.spadia.cz/media/2085/lessons fromthecovid-19pandemic.pdf.

The numbers of diagnosed and reported cases of infection with the SARS-CoV-2 virus causing the disease COVID-19, which grew into a global pandemic, have remained consistently low in all countries, including the Czech Republic, since May 2023, when the World Health Organization declared an end to the pandemic. However, it must be said that the measures implemented to control this infection did not meet all expectations. Although new mutations of the virus that can potentially cause disease, continue to emerge, it appears that most people have gradually learned to coexist with them. However, due to some unique properties of the SARS-CoV-2 virus and its variants, there will still be predisposed individuals who will develop illness and need hospitalization along with effective treatment to be supported and monitored by adequate laboratory tests. This article is a commentary on this issue and deals primarily with the diagnosis and care of early-phase COVID-19 patients. Author’s translation of the article into English is available at: https://www.spadia.cz/media/2085/lessons fromthecovid-19pandemic.pdf.

• MeSH
• Coronaviridae patogenita   MeSH
• COVID-19 * diagnóza   epidemiologie   imunologie   terapie   MeSH
• lidé MeSH
• SARS-CoV-2 patogenita   MeSH
• vakcíny proti COVID-19 farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH

Following the COVID-19 infection, the sternum dislocation and wound dehiscence resulted in an infection complicating the recovery of an immunosuppressed patient after bilateral lung transplantation. Anaerobic culture (96 h) of milky cloudy wound secretion resulted in the growth of pinpoint haemolytic colonies identified as Metamycoplasma hominis (formerly Mycoplasma hominis). The search for the endogenous source of the infection found the bacterium exclusively in the patient's sputum, making a possible link to donor lung M. hominis colonization. Unfortunately, the donor samples were no longer available. The wound infection was successfully treated with 17 days of clindamycin despite the continuous PCR detection of M. hominis in the sputum after the end of the treatment.

• MeSH
• antibakteriální látky terapeutické užití   MeSH
• COVID-19 diagnóza   MeSH
• hostitel s imunodeficiencí MeSH
• infekce chirurgické rány * mikrobiologie   farmakoterapie   diagnóza   MeSH
• klindamycin terapeutické užití   MeSH
• lidé středního věku MeSH
• lidé MeSH
• Mycoplasma hominis * genetika   izolace a purifikace   MeSH
• mykoplazmové infekce * mikrobiologie   diagnóza   farmakoterapie   MeSH
• SARS-CoV-2 genetika   izolace a purifikace   MeSH
• sputum mikrobiologie   MeSH
• transplantace plic * škodlivé účinky   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

BACKGROUND: Testing of pooled samples is an effective strategy for increasing testing capacity while saving resources and time. This study aimed to validate pooled testing and gather real-life data on its use for Covid-19 surveillance with a gargle lavage (GL) self-sampling strategy. METHODS: Two-stage pooled testing with pools of 6 and 12 samples was used for preventive testing of an asymptomatic population and Covid-19 surveillance in Czech schools. Both GL and nasopharyngeal swabs were used for sampling. RESULTS: In total, 61,111 samples were tested. The use of pooled testing for large-scale Covid-19 surveillance reduced consumable costs by almost 75% and increased testing capacity up to 3.8-fold compared to standard methods. RT-PCR experiments revealed a minimal loss of sensitivity (0-2.2%) when using pooled samples, enabling the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genes with Ct values >35. The minor loss of sensitivity was counterbalanced by a significantly increased throughput and the ability to substantially increase testing frequencies. CONCLUSIONS: Pooled testing is considerably more cost-effective and less time-consuming than standard testing for large-scale Covid-19 surveillance even when the prevalence of SARS-CoV-2 is fluctuating. Gargle lavage self-sampling is a non-invasive technique suitable for sample collection without a healthcare worker's assistance.

• MeSH
• COVID-19 * diagnóza   epidemiologie   MeSH
• lidé MeSH
• nazofarynx * virologie   MeSH
• odběr biologického vzorku * metody   MeSH
• SARS-CoV-2 * genetika   izolace a purifikace   MeSH
• senzitivita a specificita MeSH
• testování na COVID-19 průkazem nukleové kyseliny metody   MeSH
• testování na COVID-19 metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH