New methodologies based on the principle of "sewage epidemiology" have been successfully applied before in the detection of illegal drugs. The study describes the idea of early detection of a virus, e.g., SARS-CoV-2, in wastewater in order to focus on the area of virus occurrence and supplement the results obtained from clinical examination. By monitoring temporal variation in viral loads in wastewater in combination with other analysis, a virus outbreak can be detected and its spread can be suppressed early. The use of biosensors for virus detection also seems to be an interesting application. Biosensors are highly sensitive, selective, and portable and offer a way for fast analysis. This manuscript provides an overview of the current situation in the area of wastewater analysis, including genetic sequencing regarding viral detection and the technological solution of an early warning system for wastewater monitoring based on biosensors.

Biomedical Research Center of the Slovak Academy of Sciences Institute of Virology Dúbravská cesta 9 845 05 Bratislava Slovakia

Department of Environmental Engineering Institute of Chemical and Environmental Engineering Faculty of Chemical and Food Technology Slovak University of Technology Radlinského 9 812 37 Bratislava Slovakia

Department of Inorganic Technology Faculty of Chemical and Food Technology Slovak University of Technology Radlinského 9 812 37 Bratislava Slovakia

Department of Zoology and Fisheries Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Prague Kamýcká 129 165 21 Prague Czech Republic

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