Tissue homeostasis mainly depends on the activity of stem cells to replace damaged elements and restore tissue functions. Within this context, mesenchymal stem cells and fibroblasts are essential for maintaining tissue homeostasis in skin, in particular in the dermis. Modifications in collagen fibers are able to affect stem cell features. Skin properties can be significantly reduced after injuries or with aging, and stem cell niches, mainly comprising extracellular matrix (ECM), may be compromised. To this end, specific molecules can be administrated to prevent the aging process induced by UV exposure in the attempt to maintain a youngness phenotype. NanoPCL-M is a novel nanodevice able to control delivery of Mediterranean plant myrtle (Myrtus communis L.) extracts. In particular, we previously described that myrtle extracts, rich in bioactive molecules and nutraceuticals, were able to counteract senescence in adipose derived stem cells. In this study, we analyzed the effect of NanoPCL-M on skin stem cells (SSCs) and dermal fibroblasts in a dynamic cell culture model in order to prevent the effects of UV-induced senescence on proliferation and collagen depot. The BrdU assay results highlight the significantly positive effect of NanoPCL-M on the proliferation of both fibroblasts and SSCs. Our results demonstrate that-M is able to preserve SSCs features and collagen depot after UV-induced senescence, suggesting their capability to retain a young phenotype.

Center for Developmental Biology and Reprogramming Department of Biomedical Sciences University of Sassari Viale San Pietro 43 B 07100 Sassari Italy

Department of Agriculture University of Sassari Via De Nicola 9 07100 Sassari Italy

Department of Biomedical Sciences University of Sassari Viale San Pietro 43 B 07100 Sassari Italy

Department of Life and Environmental Sciences University of Cagliari University Campus 09042 Monserrato Italy

Department of Medical and Clinical Biophysics Faculty of Medicine Pavol Jozef Šafárik University Trieda SNP 1 04011 Košice Slovakia

Department of Medical Surgical and Experimental Sciences University of Sassari 07100 Sassari Italy

Institute of Biophysics 2nd Faculty of Medicine Charles University 5 Uvalu 84 150 06 Prague 5 Czech Republic

Istituto di Scienze delle Produzioni Alimentari Traversa la Crucca 3 07100 Sassari Italy

Laboratory of Molecular Biology and Stem Cell Engineering Eldor Lab National Institute of Biostructures and Biosystems Innovation Accelerator CNR Via Piero Gobetti 101 40129 Bologna Italy

UCEEB Czech Technical University Trinecka 1024 273 43 Bustehrad Czech Republic

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Effect of Helichrysum italicum in Promoting Collagen Deposition and Skin Regeneration in a New Dynamic Model of Skin Wound Healing

Electrospun Nanofibers Encapsulated with Natural Products: A Novel Strategy to Counteract Skin Aging

Myrtle-Functionalized Nanofibers Modulate Vaginal Cell Population Behavior While Counteracting Microbial Proliferation