MicroRNAs mediated regulation of glutathione peroxidase 7 expression and its changes during adipogenesis
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
34339889
DOI
10.1016/j.bbagrm.2021.194734
PII: S1874-9399(21)00052-3
Knihovny.cz E-resources
- Keywords
- Gene regulation, Glutathione peroxidase, miRNA,
- MeSH
- 3' Untranslated Regions MeSH
- Adipogenesis genetics MeSH
- Glutathione Peroxidase MeSH
- Stem Cells metabolism MeSH
- Cells, Cultured MeSH
- Middle Aged MeSH
- Humans MeSH
- RNA, Messenger metabolism MeSH
- MicroRNAs metabolism MeSH
- Cell Line, Tumor MeSH
- Peroxidases genetics MeSH
- Gene Expression Regulation, Enzymologic * MeSH
- Adipocytes metabolism MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 3' Untranslated Regions MeSH
- Glutathione Peroxidase MeSH
- GPX7 protein, human MeSH Browser
- RNA, Messenger MeSH
- MicroRNAs MeSH
- Peroxidases MeSH
Glutathione peroxidase 7 (GPx7) acts as an intracellular stress sensor/transmitter and plays an important role in adipocyte differentiation and the prevention of obesity related pathologies. For this reason, finding the regulatory mechanisms that control GPx7 expression is of great importance. As microRNAs (miRNAs) could participate in the regulation of GPx7 expression, we studied the inhibition of GPx7 expression by four selected miRNAs with relation to obesity and adipogenesis. The effect of the transfection of selected miRNAs mimics on GPx7 expression was tested in three cell models (HEK293, SW480, AT-MSC). The interaction of selected miRNAs with the 3'UTR of GPx7 was followed up on using a luciferase gene reporter assay. In addition, the levels of GPx7 and selected miRNAs in adipose tissue mesenchymal stem cells (AT-MSC) and mature adipocytes from four human donors were compared, with the changes in these levels during adipogenesis analyzed. Our results show for the first time that miR-137 and miR-29b bind to the 3'UTR region of GPx7 and inhibit the expression of this enzyme at the mRNA and protein level in all the human cells tested. However, no negative correlation between miR-137 nor miR-29b level and GPx7 was observed during adipogenesis. Despite the confirmed inhibition of GPx7 expression by miR-137 and miR-29b, the action of these two molecules in adipogenesis and mature adipocytes must be accompanied by other regulators.
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