The significant issue affecting wastewater treatment is human faeces containing SARS-CoV-2. SARS-CoV-2, as a novel coronavirus, has expanded globally. While the current focus on the COVID-19 epidemic is rightly on preventing direct transmission, the risk of secondary transmission via wastewater should not be overlooked. Many researchers have demonstrated various methods and tools for preventing and declining this virus in wastewater treatment, especially for SARS-CoV-2 in human faeces. This research reports two people tested for 30 d, with written consent, at Mosa-Ebne-Jafar Hospital of Quchan, Iran, from September 1st to October 9th, 2021. The two people's conditions are the same. The Hyssop plant was used, which boosts the immune system's effectiveness and limonene, rosemary, caffeic acids and flavonoids, all biologically active compounds in this plant, cause improved breathing problems, colds, and especially for SARS-CoV-2. As a result, utilising the Hyssop plant can help in reducing SARS-CoV-2 in faeces. This plant's antioxidant properties effectively reduce SARS-CoV-2 in faeces by 30%; nevertheless, depending on the patient's condition. This plant is also beneficial for respiratory and digestive health.

College of Engineering Department of Chemical Engineering King Khalid University Abha 61411 Saudi Arabia

Department of Chemical Engineering University of Science and Technology of Mazandaran P O Box 48518 78195 Behshahr Iran

Faculty of Science and Technology School of Applied Physics University Kebangsaan Malaysia 43600 Bangi Selangor Malaysia; International Research Centre of Nanotechnology for Himalayan Sustainability Shoolini University Solan 173212 Himachal Pradesh India; Department of Chemical Engineering School of Mining Metallurgy and Chemical Engineering University of Johannesburg P O Box 17011 Doornfontein 2028 South Africa

Institute of Environmental Engineering National Sun Yat Sen University Kaohsiung 804 Taiwan; The International University of Management Centre for Environmental Studies Main Campus Dorado Park Ext 1 Windhoek Namibia

School of Chemical Engineering Zhengzhou University Zhengzhou 450001 China

Sustainable Process Integration Laboratory SPIL NETME Centre Faculty of Mechanical Engineering Brno University of Technology VUT Brno Technická 2896 2 616 00 Brno Czech Republic

Sustainable Process Integration Laboratory SPIL NETME Centre Faculty of Mechanical Engineering Brno University of Technology VUT Brno Technická 2896 2 616 00 Brno Czech Republic; Chemical Engineering Department COMSATS University Islamabad Lahore Campus Lahore Punjab 54000 Pakistan

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Albastaki A., Naji M., Lootah R., Almeheiri R., Almulla H., Almarri I., Alreyami A., Aden A., Alghafri R. First confirmed detection of SARS-COV-2 in untreated municipal and aircraft wastewater in Dubai, UAE: the use of wastewater based epidemiology as an early warning tool to monitor the prevalence of COVID-19. Sci. Total Environ. 2021;760 PubMed PMC

Brahim Belhaouari D., Wurtz N., Grimaldier C., Lacoste A., Pires de Souza G.A., Penant G., Hannat S., La Scola B. Microscopic Observation of SARS-Like Particles in RT-qPCR SARS-CoV-2 Positive Sewage Samples. Pathogens. 2021;10(5):516. PubMed PMC

Charles D.J. Springer; 2012. Hyssop. Antioxidant Properties of Spices, Herbs and Other Sources; pp. 353–356.

Chen Y., Chen L., Deng Q., Zhang G., Wu K., Ni L., Yang Y., Liu B., Wang W., Wei C. The presence of SARS‐CoV‐2 RNA in the feces of COVID‐19 patients. J. Med. Virol. 2020;92:833–840. PubMed

Cheung K.S., Hung I.F., Chan P.P., Lung K., Tso E., Liu R., Ng Y., Chu M.Y., Chung T.W., Tam A.R. Gastrointestinal manifestations of SARS-CoV-2 infection and virus load in fecal samples from a Hong Kong cohort: systematic review and meta-analysis. Gastroenterology. 2020;159:81–95. PubMed PMC

Elsamadony M., Fujii M., Miura T., Watanabe T. Possible transmission of viruses from contaminated human feces and sewage: implications for SARS-CoV-2. Sci. Total Environ. 2021;755 PubMed PMC

Hemalatha M., Kiran U., Kuncha S.K., Kopperi H., Gokulan C., Mohan S.V., Mishra R.K. Surveillance of SARS-CoV-2 spread using wastewater-based epidemiology: comprehensive study. Sci. Total Environ. 2021;768 PubMed PMC

Hoffmann T., Alsing J. Faecal shedding models for SARS-CoV-2 RNA amongst hospitalised patients and implications for wastewater-based epidemiology. medRxiv. 2021

Jangi F., Ebadi M.-T., Ayyari M. Qualitative changes in hyssop (Hyssopus officinalis L.) as affected by cold plasma, packaging method and storage duration. J. Appl. Res. Med. Aromat. Plants. 2021;22

Lai C.C., Shih T.P., Ko W.C., Tang H.J., Hsueh P.R. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19): The epidemic and the challenges. Int. J. Antimicrob. Agents. 2020;55(3):105924. PubMed PMC

LaTurner Z.W., Zong D.M., Kalvapalle P., Gamas K.R., Terwilliger A., Crosby T., Ali P., Avadhanula V., Santos H.H., Weesner K. Evaluating recovery, cost, and throughput of different concentration methods for SARS-CoV-2 wastewater-based epidemiology. Water Res. 2021;197:117043. PubMed PMC

Li Y., Hu Y., Yu Y., Zhang X., Li B., Wu J., Li J., Wu Y., Xia X., Tang H. Positive result of Sars‐Cov‐2 in faeces and sputum from discharged patients with COVID‐19 in Yiwu, China. J. Med. Virol. 2020;92:1938–1947. PubMed PMC

Li X., Zhang S., Shi J., Luby S.P., Jiang G. Uncertainties in estimating SARS-CoV-2 prevalence by wastewater-based epidemiology. Chem. Eng. J. 2021:129039. PubMed PMC

Lin J.-H., Chen S.-J., Lee J.-E., Chu W.-Y., Yu C.-J., Chang C.-C., Chen C.-F. The detection of mercury (II) ions using fluorescent gold nanoclusters on a portable paper-based device. Chem. Eng. J. 2021

Lin L., Jiang X., Zhang Z., Huang S., Zhang Z., Fang Z., Gu Z., Gao L., Shi H., Mai L. Gastrointestinal symptoms of 95 cases with SARS-CoV-2 infection. Gut. 2020;69:997–1001. PubMed

Ng O.T., Marimuthu K., Koh V., Pang J., Linn K.Z., Sun J., De Wang L., Chia W.N., Tiu C., Chan M. SARS-CoV-2 seroprevalence and transmission risk factors among high-risk close contacts: a retrospective cohort study. Lancet Infect. Dis. 2021;21:333–343. PubMed PMC

Pal M., Berhanu G., Desalegn C., Kandi V. Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2): an update. Cureus. 2020;12(3) PubMed PMC

Pavilonis B., Ierardi A.M., Levine L., Mirer F., Kelvin E.A. Estimating aerosol transmission risk of SARS-CoV-2 in New York City public schools during reopening. Environ. Res. 2021;195:110805. PubMed PMC

Port J.R., Yinda C.K., Owusu I.O., Holbrook M., Fischer R., Bushmaker T., Avanzato V.A., Schulz J.E., Martens C., van Doremalen N. SARS-CoV-2 disease severity and transmission efficiency is increased for airborne compared to fomite exposure in Syrian hamsters. Nat. Commun. 2021;12(1):1–15. PubMed PMC

Prado T., Fumian T.M., Mannarino C.F., Resende P.C., Motta F.C., Eppinghaus A.L.F., do Vale V.H.C., Braz R.M.S., de Andrade J.d.S.R., Maranhão A.G. Wastewater-based epidemiology as a useful tool to track SARS-CoV-2 and support public health policies at municipal level in Brazil. Water Res. 2021;191 PubMed PMC

Qiu Y., Yu J., Pabbaraju K., Lee B.E., Gao T., Ashbolt N.J., Hrudey S.E., Diggle M., Tipples G., Maal-Bared R. Science of The Total Environment; 2021. Validating and Optimizing the Method for Molecular Detection and Quantification of SARS-CoV-2 in Wastewater. PubMed PMC

Ren X., Shao X.-X., Li X.-X., Jia X.-H., Song T., Zhou W.-Y., Wang P., Li Y., Wang X.-L., Cui Q.-H. Identifying potential treatments of COVID-19 from Traditional Chinese Medicine (TCM) by using a data-driven approach. J. Ethnopharmacol. 2020;258 PubMed PMC

Roussel M., Ferrant J., Reizine F., Le Gallou S., Dulong J., Carl S., Lesouhaitier M., Gregoire M., Bescher N., Verdy C. Comparative immune profiling of acute respiratory distress syndrome patients with or without SARS-CoV-2 infection. Cell Reports Medicine. 2021:100291. PubMed PMC

Serra-Compte A., González S., Arnaldos M., Berlendis S., Courtois S., Loret J.F., Schlosser O., Yáñez A.M., Soria-Soria E., Fittipaldi M. Elimination of SARS-CoV-2 along wastewater and sludge treatment processes. Water Res. 2021;202:117435. PubMed PMC

Teymoorian T., Teymourian T., Kowsari E., Ramakrishna S. Direct and indirect effects of SARS-CoV-2 on wastewater treatment. J. Water Proc. Eng. 2021;42 PubMed PMC

Wang H., Li X., Li T., Zhang S., Wang L., Wu X., Liu J. European Journal of Clinical Microbiology & Infectious Diseases; 2020. The Genetic Sequence, Origin, and Diagnosis of SARS-CoV-2; pp. 1–7. PubMed PMC

Wang R., Simoneau C.R., Kulsuptrakul J., Bouhaddou M., Travisano K.A., Hayashi J.M., Carlson-Stevermer J., Zengel J.R., Richards C.M., Fozouni P. Genetic screens identify host factors for SARS-CoV-2 and common cold coronaviruses. Cell. 2021;184(1):106–119. e114. PubMed PMC

Wang W., Xu Y., Gao R., Lu R., Han K., Wu G., Tan W. Detection of SARS-CoV-2 in different types of clinical specimens. JAMA. 2020;323:1843–1844. PubMed PMC

Wu Y., Guo C., Tang L., Hong Z., Zhou J., Dong X., Yin H., Xiao Q., Tang Y., Qu X. Prolonged presence of SARS-CoV-2 viral RNA in faecal samples. Lancet Gastroenterol. Hepatol. 2020;5:434–435. PubMed PMC

Wu J., Wang Z., Lin Y., Zhang L., Chen J., Li P., Liu W., Wang Y., Yao C., Yang K. Technical framework for wastewater-based epidemiology of SARS-CoV-2. Sci. Total Environ. 2021;791:148271. PubMed PMC

Wurtzer S., Waldman P., Ferrier-Rembert A., Frenois-Veyrat G., Mouchel J.-M., Boni M., Maday Y., Marechal V., Moulin L. Several forms of SARS-CoV-2 RNA can be detected in wastewaters: implication for wastewater-based epidemiology and risk assessment. Water Res. 2021;198 PubMed PMC

Xia B., Shen X., He Y., Pan X., Liu F.L., Wang Y., Yang F., Fang S., Wu Y., Duan Z. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021:1–14. PubMed PMC

Xiao F., Tang M., Zheng X., Liu Y., Li X., Shan H. Evidence for gastrointestinal infection of SARS-CoV-2. Gastroenterology. 2020;158:1831–1833. e1833. PubMed PMC

Xu Y., Li X., Zhu B., Liang H., Fang C., Gong Y., Guo Q., Sun X., Zhao D., Shen J. Characteristics of pediatric SARS-CoV-2 infection and potential evidence for persistent fecal viral shedding. Nat. Med. 2020;26:502–505. PubMed PMC

Zahedi A., Monis P., Deere D., Ryan U. Wastewater-based epidemiology—surveillance and early detection of waterborne pathogens with a focus on SARS-CoV-2, Cryptosporidium and Giardia. Parasitol. Res. 2021:1–22. PubMed PMC

Zahmatkesh S., Amesho K.T., Sillanpaa M., Wang C. Integration of renewable energy in wastewater treatment during COVID-19 pandemic: Challenges, opportunities, and progressive research trends. Clean. Chem. Eng. 2022;100036

Zahmatkesh, S. and M. Sillanpää (2022). Review of method and a new tool for decline and inactive SARS-CoV-2 in wastewater treatment. Clean. Chem. Eng.: 100037.

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