Most dairy calves are housed individually in early ontogeny but social housing has positive effects on calf welfare including an advantage of social buffering, i.e., when negative effects of stress are mitigated through social support of conspecific. The effects of social buffering has not yet been examined in relation to disbudding; a painful husbandry procedure commonly performed on young dairy calves. The objective of this study was to investigate the effect of pair versus individual housing on calves' behavioral reaction to disbudding. In total 52 female calves were randomly allocated either to individual (n = 16) or pair housing (n = 36, 18 focal). Calves were hot-iron disbudded with a local anesthetic and their spontaneous behavior in home pens was recorded for 24 h pre- and post-disbudding. Eating forage, ruminating, resting, exploration, play, self-grooming, and pain-related behaviors were quantified during eight 20 min intervals during the 24 h periods pre- as well as post-disbudding. In pair-housed (PAIR) calves social resting, active and passive allo-grooming were additionally recorded. The differences between individually housed (INDI, n = 10) and PAIR calves (n = 12) were tested by general linear models. The changes in pre- and post-disbudding behaviors in all calves as well as in social behaviors of PAIR calves were tested by paired t-test. We found that head shaking (t = - 3.46, P = 0.0024), head rubbing (t = 4.96, P < 0.0001) and self-grooming (t = 2.11, P = 0.04) increased in all calves after disbudding. Eating forage increased only in PAIR calves (t = 2.50, P = 0.030) which also resulted in a difference between treatments with PAIR calves fed more often than INDI calves (F1,18 = 12.96, P = 0.002). Differences in eating forage may be an indication of improved ability of PAIR calves to recover from disbudding. No other significant differences were detected between treatment groups which might have been caused by our limited sample. Our results provide the first evidence that housing treatment affects calves' reactions to disbudding, with possible indication of social buffering.

Central Veterinary Administration of the State Veterinary Administration Slezská 100 7 120 00 Prague 2 Czech Republic

Department of Ethology and Companion Animal Science Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Kamýcká 129 165 00 Prague 6 Czech Republic

Department of Ethology Institute of Animal Science Přátelství 815 104 00 Prague Uhřínevěs Czech Republic

Department of Veterinary Sciences Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Kamýcká 129 165 00 Prague 6 Czech Republic

School of Biological Sciences Institute for Global Food Security Queen's University Belfast 19 Chlorine Gardens BT9 5DL Belfast Northern Ireland UK

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Stilwell G, Lima MS, Carvalho RC, Broom DM. Effects of hot-iron disbudding, using regional anaesthesia with and without carprofen, on cortisol and behaviour of calves. Res. Vet. Sci. 2012;92:338–341. doi: 10.1016/j.rvsc.2011.02.005. PubMed DOI

Cozzi G, et al. Dehorning of cattle in the EU Member States: A quantitative survey of the current practices. Livest. Sci. 2015;179:4–11. doi: 10.1016/j.livsci.2015.05.011. DOI

Staněk S, Šarová R, Nejedlá E, Šlosárková S, Doležal O. Survey of disbudding practice on Czech dairy farms. J. Dairy Sci. 2018;101:830–839. doi: 10.3168/jds.2017-13143. PubMed DOI

Adcock SJJ, Tucker CB. Conditioned place preference reveals ongoing pain in calves 3 weeks after disbudding. Sci. Rep. 2020;10:3849. doi: 10.1038/s41598-020-60260-7. PubMed DOI PMC

Adcock SJJ, Tucker CB. The effect of disbudding age on healing and pain sensitivity in dairy calves. J. Dairy Sci. 2018;101:10361–10373. doi: 10.3168/jds.2018-14987. PubMed DOI

Gingerich KN, Choulet V, Miller-Cushon EK. Disbudding affects use of a shelter provided to group-housed dairy calves. J. Dairy Sci. 2020;103:10519–10529. doi: 10.3168/jds.2020-18267. PubMed DOI

Herskin MS, Nielsen BH. Welfare effects of the use of a combination of local anesthesia and NSAID for disbudding analgesia in dairy calves-reviewed across different welfare concerns. Front. Vet. Sci. 2018;5:16. doi: 10.3389/fvets.2018.00117. PubMed DOI PMC

USDA . Dairy 2014: Health and Management Practices on U.S. Dairy Operations, 2014. USDA; 2018.

Winder CB, et al. Canadian National Dairy Study: Heifer calf management. J. Dairy Sci. 2018;101:1056–10579. PubMed

Kiyokawa Y, Hennessy MB. Comparative studies of social buffering: A consideration of approaches, terminology, and pitfalls. Neurosci. Biobehav. Rev. 2018;86:131–141. doi: 10.1016/j.neubiorev.2017.12.005. PubMed DOI PMC

Broom DM, Leaver JD. Effects of group-rearing or partial isolation on later social-behavior of calves. Anim. Behav. 1978;26:1255–1263. doi: 10.1016/0003-3472(78)90116-1. DOI

Staněk S, Zink V, Doležal O, Štolc L. Survey of preweaning dairy calf-rearing practices in Czech dairy herds. J. Dairy Sci. 2014;97:3973–3981. doi: 10.3168/jds.2013-7325. PubMed DOI

Marcé C, Guatteo R, Bareille N, Fourichon C. Dairy calf housing systems across Europe and risk for calf infectious diseases. Animal. 2010;4:1588–1596. doi: 10.1017/S1751731110000650. PubMed DOI

Costa JHC, von Keyserlingk MAG, Weary DM. Invited review: Effects of group housing of dairy calves on behavior, cognition, performance, and health. J. Dairy Sci. 2016;99:2453–2467. doi: 10.3168/jds.2015-10144. PubMed DOI

Bernal-Rigoli JC, et al. Effects of housing and feeding systems on performance of neonatal Holstein bull calves. J. Anim. Sci. 2012;90:2818–2825. doi: 10.2527/jas.2011-4722. PubMed DOI

Valníčková B, Stěhulová I, Šárová R, Špinka M. The effect of age at separation from the dam and presence of social companions on play behavior and weight gain in dairy calves. J. Dairy Sci. 2015;98:5545–5556. doi: 10.3168/jds.2014-9109. PubMed DOI

Bučková K, Špinka M, Hintze S. Pair housing makes calves more optimistic. Sci. Rep. 2019;9:20246. doi: 10.1038/s41598-019-56798-w. PubMed DOI PMC

Bolt SL, Boyland NK, Mlynski DT, James R, Croft DP. Pair housing of dairy calves and age at pairing: Effects on weaning stress, health, production and social networks. PLoS ONE. 2017;12:0166926. doi: 10.1371/journal.pone.0166926. PubMed DOI PMC

De Paula Vieira A, von Keyserlingk MAG, Weary DM. Effects of pair versus single housing on performance and behavior of dairy calves before and after weaning from milk. J. Dairy Sci. 2010;93:3079–3085. doi: 10.3168/jds.2009-2516. PubMed DOI

Muangman P, et al. Social support correlates with survival in patients with massive burn injury. J. Burn Care Res. 2005;26:352–356. doi: 10.1097/01.BCR.0000169894.37249.4D. PubMed DOI

Pyter LM, Yang L, da Rocha JM, Engeland CJ. The effects of social isolation on wound healing mechanisms in female mice. Physiol. Behav. 2014;127:64–70. doi: 10.1016/j.physbeh.2014.01.008. PubMed DOI

Detillion CE, Craft TKS, Glasper ER, Prendergast BJ, DeVries AC. Social facilitation of wound healing. Psychoneuroendocrinology. 2004;29:1004–1011. doi: 10.1016/j.psyneuen.2003.10.003. PubMed DOI

Glasper ER, DeVries AC. Social structure influences effects of pair-housing on wound healing. Brain Behav. Immun. 2005;19:61–68. doi: 10.1016/j.bbi.2004.03.002. PubMed DOI

Dunlap KD, Vergara MM, Corbo JH. Reduced brain cell proliferation following somatic injury is buffered by social interaction in electric fish, Apteronotus leptorhynchus. Dev. Neurobiol. 2020;80:168–177. doi: 10.1002/dneu.22760. PubMed DOI

Kanitz E, Hameister T, Tuchscherer M, Tuchscherer A, Puppe B. Social support attenuates the adverse consequences of social deprivation stress in domestic piglets. Hormones Behav. 2014;65:203–210. doi: 10.1016/j.yhbeh.2014.01.007. PubMed DOI

Tuchscherer M, Kanitz E, Puppe B, Hameister T, Tuchscherer A. Social support modulates splenocyte glucocorticoid sensitivity in piglets exposed to social deprivation stress. Physiol. Behav. 2014;131:25–32. doi: 10.1016/j.physbeh.2014.04.010. PubMed DOI

Sutherland MA, Lowe GL, Huddart FJ, Waas JR, Stewart M. Measurement of dairy calf behavior prior to onset of clinical disease and in response to disbudding using automated calf feeders and accelerometers. J. Dairy Sci. 2018;101:8208–8216. doi: 10.3168/jds.2017-14207. PubMed DOI PMC

Doherty TJ, et al. Effects of a concentrated lidocaine solution on the acute phase stress response to dehorning in dairy calves. J. Dairy Sci. 2007;90:4232–4239. doi: 10.3168/jds.2007-0080. PubMed DOI

Grondahl-Nielsen C, Simonsen HB, Lund JD, Hesselholt M. Behavioural, endocrine and cardiac responses in young calves undergoing dehorning without and with use of sedation and analgesia. Vet. J. 1999;158:14–20. doi: 10.1053/tvjl.1998.0284. PubMed DOI

Martin MS, et al. Effect of bupivacaine liposome suspension administered as a cornual nerve block on indicators of pain and distress during and after cautery dehorning in dairy calves. J. Diary Sci. 2022;105:1603–1617. doi: 10.3168/jds.2021-21004. PubMed DOI

Adcock SJJ, Tucker CB. Injury alters motivational trade-ofs in calves during the healing period. Sci. Rep. 2021;11:6888. doi: 10.1038/s41598-021-86313-z. PubMed DOI PMC

Melendéz DM, Marti S, Haley DB, Schwinghamer TM, Schwartzkopf-Genswein KS. Effect of transport and rest stop duration on the welfare of conditioned cattle transported by road. PLoS ONE. 2020;15:e0228492. doi: 10.1371/journal.pone.0228492. PubMed DOI PMC

Llonch P, et al. Association of temperament and acute stress responsiveness with productivity, feed efficiency, and methane emissions in beef cattle: An observational study. Front. Vet. Sci. 2016 doi: 10.3389/fvets.2016.00043. PubMed DOI PMC

Phillips HN, Heins BJ. Evaluation of an herbal therapy to alleviate acute pain and stress of disbudded dairy calves under organic management. Transl. Anim. Sci. 2021;5:044. doi: 10.1093/tas/txab044. PubMed DOI PMC

Winder CB, et al. Clinical trial of local anesthetic protocols for acute pain associated with caustic paste disbudding in dairy calves. J. Dairy Sci. 2017;100:6429–6441. doi: 10.3168/jds.2017-12724. PubMed DOI

Reedman CN, et al. Randomized control trial assessing the efficacy of pain control strategies for caustic paste disbudding in dairy calves younger than 9 days of age. J. Dairy Sci. 2020;103:7339–7350. doi: 10.3168/jds.2019-18118. PubMed DOI

Mirra A, Spadavecchia C, Bruckmaier R, Gutzwiller A, Casoni D. Acute pain and peripheral sensitization following cautery disbudding in 1- and 4-week-old calves. Physiol. Behav. 2018;184:248–260. doi: 10.1016/j.physbeh.2017.11.031. PubMed DOI

Færevik G, Jensen MB, Bøe KE. Dairy calves social preferences and the significance of a companion animal during separation from the group. Appl. Anim. Behav. Sci. 2006;99:205–221. doi: 10.1016/j.applanim.2005.10.012. DOI

Mintline EM, et al. Play behavior as an indicator of animal welfare: Disbudding in dairy calves. Appl. Anim. Behav. Sci. 2013;144:22–30. doi: 10.1016/j.applanim.2012.12.008. DOI

Rushen J, de Passillé AM. Automated measurement of acceleration can detect effects of age, dehorning and weaning on locomotor play of calves. Appl. Anim. Behav. Sci. 2012;139:169–174. doi: 10.1016/j.applanim.2012.04.011. DOI

Faulkner PM, Weary DM. Reducing pain after dehorning in dairy calves. J. Dairy Sci. 2000;83:2037–2041. doi: 10.3168/jds.S0022-0302(00)75084-3. PubMed DOI

Adcock SJJ, Cruz DM, Tucker CB. Behavioral changes in calves 11 days after cautery disbudding: Effect of local anesthesia. J. Dairy Sci. 2020;103:8518–8525. doi: 10.3168/jds.2020-18337. PubMed DOI

Heinrich A, Duffield TF, Lissemore KD, Millman ST. The effect of meloxicam on behavior and pain sensitivity of dairy calves following cautery dehorning with a local anesthetic. J. Dairy Sci. 2010;93:2450–2457. doi: 10.3168/jds.2009-2813. PubMed DOI

Winder CB, et al. Effects of local anesthetic or systemic analgesia on pain associated with cautery disbudding in calves: A systematic review and meta-analysis. J. Diary Sci. 2018;101:5411. doi: 10.3168/jds.2017-14092. PubMed DOI

Graf B, Senn M. Behavioural and physiological responses of calves to dehorning by heat cauterization with or without local anaesthesia. Appl. Anim. Behav. Sci. 1999;62:153–171. doi: 10.1016/S0168-1591(98)00218-4. DOI

Stafford KJ, Mellor DJ. The assessment of pain in cattle: A review. CAB Rev. Perspect. Agric. Vet. Sci. Nutr. Nat. Resour. 2006;1:10.

Ede T, von Keyserlingk MAG, Weary DM. Social approach and place aversion in relation to conspecific pain in dairy calves. PLoS ONE. 2020;15:e0232897. doi: 10.1371/journal.pone.0232897. PubMed DOI PMC

Turner SP, McIlvaney K, Donbavand J, Turner MJ. The effect of behavioural indicators of calf discomfort following routine procedures on cow maternal care. Animals (Basel) 2020;10:87. doi: 10.3390/ani10010087. PubMed DOI PMC

Altmann J. Observational study of behaviour—Sampling methods. Behaviour. 1974;49:227–267. doi: 10.1163/156853974X00534. PubMed DOI

Loberg JM, et al. Weaning and separation in two steps—A way to decrease stress in dairy calves suckled by foster cows. Appl. Anim. Behav. Sci. 2008;111:222–234. doi: 10.1016/j.applanim.2007.06.011. DOI

Jensen MB, Kyhn R. Play behaviour in group-housed dairy calves, the effect of space allowance. Appl. Anim. Behav. Sci. 2000;67:35–46. doi: 10.1016/S0168-1591(99)00113-6. PubMed DOI

Milligan BN, Duffield T, Lissemore K. The utility of ketoprofen for alleviating pain following dehorning in young dairy calves. Can. Vet. J. 2004;45:140–143. PubMed PMC

Færevik G, Tjentland K, Løvik S, Andersen IL, Bøe KE. Resting pattern and social behaviour of dairy calves housed in pens with different sized lying areas. Appl. Anim. Behav. Sci. 2008;114:54–64. doi: 10.1016/j.applanim.2008.01.002. DOI

Rhoads ML, et al. Effects of heat stress and plane of nutrition on lactating Holstein cows: I. Production, metabolism, and aspects of circulating somatotropin. J. Dairy Sci. 2009;92:1986–1997. doi: 10.3168/jds.2008-1641. PubMed DOI

Roesch Ely D, et al. Effect of restraint stress on feeding behavior of rats. Physiol. Behav. 1997;61:395–398. doi: 10.1016/S0031-9384(96)00450-7. PubMed DOI

Leal E, Fernández-Durán B, Guillot R, Ríos D, Cerdá-Reverter JM. Stress-induced effects on feeding behavior and growth performance of the sea bass (Dicentrarchus labrax): A self-feeding approach. J. Comp. Physiol. B. 2011;118:1035–1044. doi: 10.1007/s00360-011-0585-z. PubMed DOI

Theurer M, et al. Assessment of behavioral changes associated with oral meloxicam administration at time of dehorning in calves using a remote triangulation device and accelerometers. BMC Vet. Res. 2012;8:48. doi: 10.1186/1746-6148-8-48. PubMed DOI PMC

Hixson CL, Krawczel PD, Caldwell JM, Miller-Cushon EK. Behavioral changes in group-housed dairy calves infected with Mannheimia haemolytica. J. Dairy Sci. 2018;101:10351–10360. doi: 10.3168/jds.2018-14832. PubMed DOI

Proudfoot KL, Jensen MB, Weary DM, von Keyserlingk MAG. Dairy cows seek isolation at calving and when ill. J. Dairy Sci. 2014;97:2731–2739. doi: 10.3168/jds.2013-7274. PubMed DOI

Kikusui T, Winslow JT, Mori Y. Social buffering: Relief from stress and anxiety. Philos. Trans. R. Soc. Lond. B Biol. Sci. 2006;361:2215–2228. doi: 10.1098/rstb.2006.1941. PubMed DOI PMC

Harding EJ, Paul ES, Mendl M. Animal behavior: Cognitive bias and affective state. Nature. 2004;427:312. doi: 10.1038/427312a. PubMed DOI

Raussi S, et al. The formation of preferential relationships at early age in cattle. Behav. Process. 2010;84:726–731. doi: 10.1016/j.beproc.2010.05.005. PubMed DOI

Lecorps B, Kappel S, Weary DM, Keyserlingk MAG. Social proximity in dairy calves is affected by differences in pessimism. PLoS ONE. 2019;14:0223746. doi: 10.1371/journal.pone.0223746. PubMed DOI PMC

Grøndahl AM, Skancke EM, Mejdell CM, Jansen JH. Growth rate, health and welfare in a dairy herd with natural suckling until 6–8 weeks of age: A case report. Acta Vet. Scand. 2007;49:16. doi: 10.1186/1751-0147-49-16. PubMed DOI PMC

Johnsen JF, et al. Is rearing calves with the dam a feasible option for dairy farms?—Current and future research. Appl. Anim. Behav. Sci. 2016;181:1–11. doi: 10.1016/j.applanim.2015.11.011. DOI