Vibrational circular dichroism (VCD) spectroscopy has been widely used to study (bio)molecules in solution. However, its solid-state applications have been restricted due to experimental limitations and artifacts. Having overcome some of them, the first VCD study of nucleoside crystals is now presented. A two-orders-of-magnitude enhancement of VCD signal was observed due to high molecular order in the crystals and resulting supramolecular chirality. This allowed to obtain high-quality VCD spectra within minutes using minute amounts of samples. The VCD technique is extremely sensitive in detecting changes in a crystal order and is able to distinguish different hydration states of crystals. This elevates it to a new level, as a fast and efficient tool to study chiral crystalline samples. This study demonstrates that VCD is capable of near-instantaneous detection of hydration polymorphs and crystal degradation, which is of substantial interest in pharmaceutical industry (quality and stability control).

3rd Faculty of Medicine Charles University Ruská 87 10000 Prague Czech Republic

Department of Biology and Biochemistry University of Bath Claverton Down Bath BA2 7AY United Kingdom

Faculty of Chemistry Jagiellonian University Gronostajowa 2 30 387 Kraków Poland

Hylleraas Centre for Quantum Molecular Sciences Department of Chemistry UiT The Arctic University of Norway N 9037 Tromsø Norway

Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Flemingovo náměstí 2 16610 Prague Czech Republic

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