We assessed the influence of diet on the development and survival in the immature stages of the necrophagous beetle Oiceoptoma thoracicum (Linnaeus, 1758). The species is frequently observed on large cadavers, including humans, and thus can be of potential forensic relevance. We compared multiple meat tissues from three animal sources, and detected the optimal diet for rearing the species for further entomological and forensic objectives. We reared 203 individuals to adulthood at the constant temperature of 20°C. Our results represent the first robust data set of the development time for this species. They further confirmed a significant relationship between survival and the type of diet, as the highest survival rates were detected in larvae fed with both pork liver and pork muscle.

Department of Ecology Faculty of Environmental Sciences Czech University of Life Sciences Prague Kamýcká 129 CZ 165 00 Praha Suchdol Czech Republic

Police of the Czech Republic Institute of Criminalistics Prague P O Box 62 KUP CZ 170 89 Praha Czech Republic

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Bambaradeniya, Y. T. B., Karunaratne W. A. I. P., Tomberlin J. K., Goonerathne I., Kotakadeniya R. B., and Magni P. A... 2019. Effect of temperature and tissue type on the development of the forensic fly Chrysomya megacephala (Diptera: Calliphoridae). J. Med. Entomol. 56: 1571–1581. PubMed

Benjamini, Y., and Hochberg Y... 1995. Controlling the false discovery rate: a practical and powerful approach to multiple testing on JSTOR. J. R. Stat. Soc. Ser. B. 57: 289–300.

Bernhardt, V., Schomerus C., Verhoff M. A., and Amendt J... 2017. Of pigs and men-comparing the development of Calliphora vicina (Diptera: Calliphoridae) on human and porcine tissue. Int. J. Legal Med. 131: 847–853. PubMed

Boatright, S. A., and Tomberlin J. K... 2010. Effects of temperature and tissue type on the development of Cochliomyia macellaria (Diptera: Calliphoridae). J. Med. Entomol. 47: 917–923. PubMed

Byrd, J. H., and Castner J. L.. 2009. Insects of forensic importance, pp. 39–126. InByrd J. H., and Castner J. L. (eds.), Forensic entomology the utility of arthropods in legal investigations. CRC Press, Boca Raton, FL.

Byrd, J., and Tomberlin J.. 2019. Laboratory-rearing of forensic insects, pp. 87–102. InByrd J., and Tomberlin J. (eds.), Forensic entomology the utility of arthropods in legal investigations. CRC Press, Boca Raton, FL.

Chapman, R. F. 2012. The insects: Structure and function, 5th ed. Cambridge University Press, Cambridge.

Charabidze, D., Gosselin M., and Hedouin V.. 2017. Use of necrophagous insects as evidence of cadaver relocation: myth or reality? PeerJ. 2017: 1–32. PubMed PMC

Clark, K., Evans L., and Wall R.. 2006. Growth rates of the blowfly, Lucilia sericata, on different body tissues. Forensic Sci. Int. 156: 145–149. PubMed

Day, D. M., and Wallman J. F.. 2006. Influence of substrate tissue type on larval growth in Calliphora augur and Lucilia cuprina (Diptera: Calliphoridae). J. Forensic Sci. 51: 657–663. PubMed

Dekeirsschieter, J., Verheggen F. J., Haubruge E., and Brostaux Y.. 2011a. Carrion beetles visiting pig carcasses during early spring in Urban, Forest and Agricultural Biotopes of Western Europe. J. Insect Sci. 11: 1–13. PubMed PMC

Dekeirsschieter, J., Verheggen F., Lognay G., and Haubruge E.. 2011b. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review. Biotechnol. Agron. Soc. Environ. 15: 435–447.

Donato, A. -D., and Liria J.. 2016. Vital Statistics of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) under Different diets from Venezuela. J. Entomol. Zool. Stud 2: 247–251.

Esh, M., and Oxbrough A.. 2021. Macrohabitat associations and phenology of carrion beetles (Coleoptera: Silphidae, Leiodidae: Cholevinae). J. Insect Conserv. 25: 123–136.

Frątczak-Łagiewska, K., Grzywacz A., and Matuszewski S.. 2020. Development and validation of forensically useful growth models for Central European population of Creophilus maxillosus L. (Coleoptera: Staphylinidae). Int. J. Legal Med. 134: 1531–1545. PubMed PMC

Haskell, N. H., Hall R. D., Cervenka V. J., and Clark M.A.. 1997. On the body: insect’s life stage presence and their post-mortem artifacts, pp. 415–441. InHaglund W. D., and Sorg M. H.. (eds.), Forensic taphonomy. post-mortem fate of human remains. CRC Press,Boca Raton FL.

Heymons, R., and von Lengerken H.. 1931. Studien über die Lebenserscheinungen der Silphini. VII. Oeceoptoma thoracica L. Zeitschrift für Morphol. und Ökologie der Tiere. 20: 691–706.

House, H. L. 1969. Effects of different proportions of nutrients on insects. Entomol. Exp. Appl. 12: 651–669.

House, H. L. 1974. Nutrition, pp. 1–62. InRockstein M., (ed.), Physiol. Insecta. Academic Press, New York.

Ireland, S., and Turner B.. 2006. The effects of larval crowding and food type on the size and development of the blowfly, Calliphora vomitoria. Forensic Sci. Int. 159: 175–181. PubMed

Jakubec, P., Novák M., Qubaiová J., Šípková H., Růžička J., and Šuláková H.. 2018. Interactive identification key of forensically important beetles species (Coleoptera). Version 1.00. doi:10.0.20.161/zenodo.3736197. https://github.com/jakubecp/id_forensic. DOI

Jakubec, P., Qubaiová J., Novák M., and Růžička J.. 2021. Developmental biology of forensically important beetle, Necrophila (Calosilpha) brunnicollis (Coleoptera: Silphidae). J. Med. Entomol. 58: 64–70. PubMed

Jakubec, P., and Růžička J.. 2015. Is the type of soil an important factor determining the local abundance of carrion beetles (Coleoptera: Silphidae)? Eur. J. Entomol. 112: 747–754.

Kaneshrajah, G., and Turner B.. 2004. Calliphora vicina larvae grow at different rates on different body tissues. Int. J. Legal Med. 118: 242–244. PubMed

Kočárek, P. 2001. Diurnal activity rhythms and niche differentiation in a carrion beetle assemblage (Coleoptera: Silphidae) in Opava, the Czech Republic. Biol. Rhythm. Res. 32: 431–438.

Kočárek, P. 2003. Decomposition and Coleoptera succession on exposed carrion of small mammal in Opava, the Czech Republic. Eur. J. Soil Biol. 39: 31–45.

Kočárek, P., and Benko K.. 1997. Výskyt a sezónní aktivita brouků čeledi Silphidae na Hlučínsku (Slezsko, Česká republika) (The occurence and seasonal activity of Silphidae in the Hlučín region (Silesia, Czech Republic)). Časopis Slezského Muz. v Opavě, Ser. A - Vědy Přírodní. 46: 173–179.

Lüdecke, D. 2020. sjPlot: data visualization for statistics in social science. doi:10.5281/zenodo.1308157. DOI

Matuszewski, S., Bajerlein D., Konwerski S., and Szpila K.. 2010. Insect succession and carrion decomposition in selected forests of Central Europe. Part 2: composition and residency patterns of carrion fauna. Forensic Sci. Int. 195: 42–51. PubMed

Matuszewski, S., Bajerlein D., Konwerski S., and Szpila K.. 2011. Insect succession and carrion decomposition in selected forests of Central Europe. Part 3: succession of carrion fauna. Forensic Sci. Int. 207: 150–163. PubMed

Matuszewski, S., and Szafałowicz M.. 2013. Temperature-dependent appearance of forensically useful beetles on carcasses. Forensic Sci. Int. 229: 92–99. PubMed

Matuszewski, S., Szafałowicz M., and Jarmusz M.. 2013. Insects colonising carcasses in open and forest habitats of Central Europe: Search for indicators of corpse relocation. Forensic Sci. Int. 231: 234–239. PubMed

Nation, J. L. 2015. Insect physiology and biochemistry. CRC Press, Boca Raton, FL.

Newton, A. F. 2020. StaphBase: staphyliniformia world catalog database (version Nov 2018). InRoskov Y., Ower G., Orrell T., Nicolson D., Bailly N., Kirk P. M., Bourgoin T., DeWalt R. E., Decock W., van Nieukerken E., and Penev L. (eds.), Species 2000 and ITIS Catalogue of Life, Digital resource at www.catalogueoflife.org. Species 2000: Naturalis, Leiden, the Netherlands. ISSN 2405–8858.

Peschke, K., Krapf D., and Fuldner D.. 1987. Ecological speration, functional relationships and limiting resources in a carrion insect community. Zool. Jahrbücher. Abteilung für Syst. Ökologie und Geogr. der Tiere. 114: 241–265.

Qubaiová, J., Jakubec P., Montoya-Molina S., Novák M., and Šuláková H.. 2021. Influence of diet on development and survival of thanatophilus rugosus (Coleoptera: Silphidae). J. Med. Entomol. 58: 2124–2129. PubMed

R Core Team. 2020. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.

Ratcliffe, B. C. 1996. The carrion beetles (Coleoptera: Silphidae) of Nebraska. Bull. Univ. Nebraska State Mus. 13: 1–100.

Ridgeway, J. A., Midgley J. M., Collett I. J., and Villet M. H.. 2014. Advantages of using development models of the carrion beetles Thanatophilus micans (Fabricius) and T. mutilatus (Castelneau) (Coleoptera: Silphidae) for estimating minimum post mortem intervals, verified with case data. Int. J. Legal Med. 128: 207–220. PubMed

Rogers, E. K. B., Franklin D., and Voss S. C.. 2021. Dietary effects on the development of Calliphora dubia and Chrysomya rufifacies (Diptera: Calliphoridae): implications for Postmortem Interval. J. Med. Entomol. 58: 79–87. PubMed

Růžička, J. 1994. Seasonal activity and habitat associations of Silphidae and Leiodidae: Cholevinae (Coleoptera) in central Bohemia. Acta Soc. Zool. Bohemicae. 58: 67–78.

Růžička, J., Háva J., and Schneider J.. 2004. Revision of Palaearctic and Oriental Oiceoptoma (Coleoptera: Silphidae). Acta Soc. Zool. Bohem. 68: 30–51.

Scriber, J. M., and Slansky F.. 1981. The nutritional ecology of immature insects. Annu. Rev. Entomol. 26: 183–211.

Tarone, A. M., and Benoit J. B.. 2019. Insect development as it relates to forensic entomology, pp. 225–252. InByrd J. H, and Tomberlin J. K., (eds.), Forensic entomology the utility of arthropods in legal investigations. CRC Press, Boca Raton, FL.

Therneau, T. M. 2021. A Package for Survival Analysis in R. R package version 3.2-12. https://CRAN.R-project.org/package=survival.

Thomas, J. K., Sanford M. R., Longnecker M., and Tomberlin J. K.. 2016. Effects of temperature and tissue type on the development of megaselia scalaris (Diptera: Phoridae). J. Med. Entomol. 53: 519–525. PubMed

Thyssen, P. J., de Souza C. M., Shimamoto P. M., Salewski T. de B., and Moretti T. C.. 2014. Rates of development of immatures of three species of Chrysomya (Diptera: Calliphoridae) reared in different types of animal tissues: implications for estimating the postmortem interval. Parasitol. Res. 113: 3373–3380. PubMed

U.S. Department of Agriculture (USDA) Agricultural Research Service. 2019. Food data central. (https://fdc.nal.usda.gov/ (Accessed 01 July 2021).

Warren, J. A., and Anderson G. S.. 2009. A comparison of development times for protophormia terraenovae (r-d) reared on different food substrates. J. Can. Soc. Forensic Sci. 42: 161–171.

Wells, J. D., and LaMotte L. R.. 2009. Estimating the Postmortem Interval, pp. 367–388. InByrd J. H., and Castner J. L. (eds.), Forensic entomology the utility of arthropods in legalinvestigations. CRC Press,Boca Raton FL.

Wickham, H. 2009. ggplot2: Elegant graphics for data analysis. Springer-Verlag, New York, New York.