This review updates the current status of activities related to hazard characterisation for mycotoxins, with special reference to regulatory work accomplished within the European Union. Because the relevant information on these topics is widely scattered in the scientific literature, this review intends to provide a condensed overview on the most pertinent aspects. Human health risk assessment is a procedure to estimate the nature and potential for harmful effects of mycotoxins on human health due to exposure to them via contaminated food. This assessment involves hazard identification, hazard characterisation, exposure assessment, and risk characterisation. Mycotoxins covered in this review are aflatoxins, ochratoxin A, cyclopiazonic acid, citrinin, trichothecenes (deoxynivalenol, nivalenol, T-2, and HT-2 toxins), fumonisins, zearalenone, patulin, and ergot alkaloids. For mycotoxins with clear genotoxic/carcinogenic properties, the focus is on the margin of exposure approach. One of its goals is to document predictive characterisation of the human hazard, based on studies in animals using conditions of low exposure. For the other, non-genotoxic toxins, individual 'no adverse effect levels' have been established, but structural analogues or modified forms may still complicate assessment. During the process of hazard characterisation, each identified effect is assessed for human relevance. The estimation of a 'safe dose' is the hazard characterisation endpoint. The final aim of all of these activities is to establish a system, which is able to minimise and control the risk for the consumer from mycotoxins in food. Ongoing research on mycotoxins constantly comes up with new findings, which may have to be implemented into this system.

Center for Health Nutrition and Food in Brno National Institute of Public Health Palackeho 3a 61242 Brno Czech Republic

Department of Biology Faculty of Science University of Hradec Kralove Rokitanskeho 62 CZ 50003 Hradec Kralove Czech Republic

The IARC Monographs Programme International Agency for Research On Cancer Lyon France

See more in PubMed

Adhikari M, Negi B, Kaushik N, Adhikari A, Al-Khedhairy AA, Kaushik NK, Choi EH (2017) T-2 mycotoxin: toxicological effects and decontamination strategies. Oncotarget 8:33933–33952. https://doi.org/10.18632/oncotarget.15422

Aziz NH, Moussa LAA (2002) Influence of gamma-radiation on mycotoxin producing moulds and mycotoxins in fruits. Food Control 13:281–288. https://doi.org/10.1016/S0956-7135(02)00028-2 DOI

Becci PJ, Hess FG, Johnson WD, Gallo MA, Babish JG, Dailey RE, Parent RA (1981) Long-term carcinogenicity and toxicity studies of patulin in the rat. J Appl Toxicol 1:256–261. https://doi.org/10.1002/jat.2550010504 PubMed DOI

Benford D, Bolger PM, Carthew P, Coulet M, DiNovi M, Leblanc J-C, Renwick AG, Setzer W, Shlatter J, Smith B, Slob W, Williams G et al (2010a) Application of the margin of exposure (MoE) approach to substances in food that are genotoxic and carcinogenic. Food Chem Toxicol 48:S2–S24. https://doi.org/10.1016/j.fct.2009.11.003 PubMed DOI

Benford D, Jean-Charles L, Setzer R (2010b) Application of the margin of exposure (MoE) approach to substances in food that are genotoxic and carcinogenic Example: Aflatoxin B1 (AFB1). Food Chem Toxicol 48:S34–S41. https://doi.org/10.1016/j.fct.2009.11.003 PubMed DOI

Bennett JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16:497–516. https://doi.org/10.1128/CMR.16.3.497-516.2003 PubMed DOI PMC

Bertero A, Moretti A, Spicer L, Caloni F (2018) Fusarium molds and mycotoxins: potential species-specific effects. Toxins 10:244. https://doi.org/10.3390/toxins10060244 PubMed DOI PMC

Bondy G (2009) Clinical and renal effects of long-term exposure to vomitoxin in wild type and p53+/í transgenic mice. In: Investigations on toxicity of deoxynivalenol in transgenic mice and in vitro—a preliminary report. Halth Canada, Ottawa, Ontario

Bondy GS, Coady L, Curran I, Caldwell D, Armstrong C, Aziz SA, Nunnikhoven A, Gannon AM, Liston V, Shenton J, Mehta R (2016) Effects of chronic deoxynivalenol exposure on p53 heterozygous and p53 homozygous mice. Food Chem Toxicol 96:24–34. https://doi.org/10.1016/j.fct.2016.07.018 PubMed DOI

Braun MS, Wink M (2018) Exposure, occurrence, and chemistry of fumonisins and their cryptic derivatives. Compr Rev Food Sci Food Saf 17:769–791. https://doi.org/10.1111/1541-4337.12334 PubMed DOI

Bryden WL (2007) Mycotoxins in the food chain: human health implications. Asia Pac J Clin Nutr 16(Suppl 1):95–101 PubMed

Bui-Klimke TR, Wu F (2015) Ochratoxin A and human health risk: a review of the evidence. Crit Rev Food Sci Nutr 55:1860–1869. https://doi.org/10.1080/10408398.2012.724480 PubMed DOI PMC

Burdock GA, Flamm WG (2000) Review article: safety assessment of the mycotoxin cyclopiazonic acid. Int J Toxicol 19:195–218. https://doi.org/10.1080/10915810050074964 DOI

Burrows GE, Tyrl RJ (2012) Poaceae Barnhart. In: Burrows, G.E. and Tyrl, R.J. (eds.) Toxic plants of North America. Wiley-Blackwell, Ames, IA, USA, pp 899–904

Carballo D, Tolosa J, Ferrer E, Berrada H (2019) Dietary exposure assessment to mycotoxins through total diet studies. A Review Food Chem Toxicol 128:8–20. https://doi.org/10.1016/j.fct.2019.03.033 PubMed DOI

CAST (ed) (2003) Mycotoxins: risks in plant, animal, and human systems. Council for Agricultural Science and Technology, Ames, Iowa

Chang P-K, Ehrlich KC, Fujii I (2009) Cyclopiazonic acid biosynthesis of Aspergillus flavus and Aspergillus oryzae. Toxins (basel) 1:74–99. https://doi.org/10.3390/toxins1020074 PubMed DOI

da Rocha MEB, da Freire F, CO, Maia FEF, Guedes MIF, Rondina D, (2014) Mycotoxins and their effects on human and animal health. Food Control 36:159–165. https://doi.org/10.1016/j.foodcont.2013.08.021 DOI

Dai Q, Zhao J, Qi X, Xu W, He X, Guo M, Dweep H, Cheng W-H, Luo Y, Xia K, Gretz N, Huang K (2014) MicroRNA profiling of rats with ochratoxin A nephrotoxicity. BMC Genomics 15:333. https://doi.org/10.1186/1471-2164-15-333 PubMed DOI PMC

De Boevre M, Jacxsens L, Lachat C, Eeckhout M, Di Mavungu JD, Audenaert K, Maene P, Haesaert G, Kolsteren P, De Meulenaer B, De Saeger S (2013) Human exposure to mycotoxins and their masked forms through cereal-based foods in Belgium. Toxicol Lett 218:281–292. https://doi.org/10.1016/j.toxlet.2013.02.016 PubMed DOI

De Waal EJ (2002) Safety assessment of cyclopiazonic acid. Int J Toxicol 21:425–427; discussion 429, 431. https://doi.org/10.1080/10915810290096658

Desjardins AE, Proctor RH (2007) Molecular biology of Fusarium mycotoxins. Int J Food Microbiol 119:47–50. https://doi.org/10.1016/j.ijfoodmicro.2007.07.024 PubMed DOI

Dragan YP, Bidlack WR, Cohen SM, Goldsworthy TL (2001) Implications of apoptosis for toxicity, carcinogenicity, and risk assessment: fumonisin B(1) as an example. Toxicol Sci 61:6–17. https://doi.org/10.1093/toxsci/61.1.6 PubMed DOI

Edwards S, Cantley TC, Rottinghaus GE, Osweiler GD, Day BN (1987) The effects of zearalenone on reproduction in swine. I. The relationship between ingested zearalenone dose and anestrus in non-pregnant, sexually mature gilts. Theriogenology 28:43–49. https://doi.org/10.1016/0093-691x(87)90184-1 PubMed DOI

Edwards SG (2009a) Fusarium mycotoxin content of UK organic and conventional wheat. Food Addit Contam Part A 26:496–506. https://doi.org/10.1080/02652030802530679 DOI

Edwards SG (2009b) Fusarium mycotoxin content of UK organic and conventional oats. Food Addit Contam Part A 26:1063–1069. https://doi.org/10.1080/02652030902788953 DOI

Edwards SG (2009c) Fusarium mycotoxin content of UK organic and conventional barley. Food Addit Contam Part A 26:1185–1190. https://doi.org/10.1080/02652030902919418 DOI

EFSA - European Food Safety Authority (2016a) EFSA strategy 2020: trusted science for safe food, Protecting consumers’ health with independent scientific advice on the food chain. Publications Office 1–29. https://doi.org/10.2805/419620

EFSA - European Food Safety Authority (2022a) The four steps of risk assessment. In: European Food Safety Authority. https://multimedia.efsa.europa.eu/riskassessment/index.htm . Accessed 15 Feb 2023

EFSA - European Food Safety Authority (2005) Opinion of the Scientific Committee on a request from EFSA related to a harmonised approach for risk assessment of substances which are both genotoxic and carcinogenic. EFSA J 1–31. https://doi.org/10.2903/j.efsa.2005.282

EFSA - European Food Safety Authority (2012a) Statement on the applicability of the margin of exposure approach for the safety assessment of impurities which are both genotoxic and carcinogenic in substances added to food/feed. EFSA J 10:2578. https://doi.org/10.2903/j.efsa.2012.2578 DOI

EFSA - European Food Safety Authority (2017a) Update: use of the benchmark dose approach in risk assessment. EFSA J 15:e04658. https://doi.org/10.2903/j.efsa.2017.4658

EFSA - European Food Safety Authority (2022b) Guidance on the use of the benchmark dose approach in risk assessment. EFSA Journal 20:e07584. https://doi.org/10.2903/j.efsa.2022.7584

EFSA - European Food Safety Authority (2020a) Risk assessment of aflatoxins in food. EFSA J 18:1–112. https://doi.org/10.2903/j.efsa.2020.6040 DOI

EFSA - European Food Safety Authority (2007) European Food Safety Authority. Opinion of the scientific panel on contaminants in the food chain [CONTAM] related to the potential increase of consumer health risk by a possible increase of the existing maximum levels for aflatoxins in almonds, hazelnuts and pistachios and derived. European Food Safety Authority 446:1–127

EFSA - European Food Safety Authority (2020b) Risk assessment of ochratoxin A in food. EFSA J 18:1–150. https://doi.org/10.2903/j.efsa.2020.6113 DOI

EFSA - European Food Safety Authority (2017b) Risks to human and animal health related to the presence of deoxynivalenol and its acetylated and modified forms in food and feed. EFSA J 15:1–345. https://doi.org/10.2903/j.efsa.2017.4718 DOI

EFSA - European Food Safety Authority (2013) Scientific opinion on risks for animal and public health related to the presence of nivalenol in food and feed. EFSA J 11:3262. https://doi.org/10.2903/j.efsa.2013.3262 DOI

EFSA - European Food Safety Authority (2017c) Human and animal dietary exposure to T-2 and HT-2 toxin. EFSA J 15:1–57. https://doi.org/10.2903/j.efsa.2017.4972 DOI

EFSA - European Food Safety Authority (2011) Scientific opinion on the risks for public health related to the presence of zearalenone in food. EFSA J 9:2197. https://doi.org/10.2903/j.efsa.2011.2197 DOI

EFSA - European Food Safety Authority (2016b) Appropriateness to set a group health-based guidance value for zearalenone and its modified forms. EFSA J 14:1–46. https://doi.org/10.2903/j.efsa.2016.4425 DOI

EFSA - European Food Safety Authority (2018) Risks for animal health related to the presence of fumonisins, their modified forms and hidden forms in feed. EFSA J 16:1–144. https://doi.org/10.2903/j.efsa.2018.5242 DOI

EFSA - European Food Safety Authority (2012b) Scientific opinion on the risks for public and animal health related to the presence of citrinin in food and feed. EFSA J 10:1–82. https://doi.org/10.2903/j.efsa.2012.2605 DOI

EFSA - European Food Safety Authority (2012c) Scientific opinion on ergot alkaloids in food and feed. EFSA Panel on Contaminants in the Food Chain (CONTAM). EFSA J 10:2798. https://doi.org/10.2903/j.efsa.2012.2798

EFSA - European Food Safety Authority (2017d) Human and animal dietary exposure to ergot alkaloids. EFSA J 15:1–53. https://doi.org/10.2903/j.efsa.2017.4902 DOI

Escrivá L, Font G, Manyes L (2015) In vivo toxicity studies of fusarium mycotoxins in the last decade: a review. Food Chem Toxicol 78:185–206. https://doi.org/10.1016/j.fct.2015.02.005 PubMed DOI

Fitzhugh OG, Nelson AA, Calvery HO (1944) The chronic toxicity of ergot. J Pharmacol Exp Ther 82:364–376

Flannery BM, Wu W, Pestka JJ (2011) Characterization of deoxynivalenol-induced anorexia using mouse bioassay. Food Chem Toxicol 49:1863–1869. https://doi.org/10.1016/j.fct.2011.05.004 PubMed DOI PMC

Fraeyman S, Croubels S, Devreese M, Antonissen G (2017) Emerging Fusarium and Alternaria mycotoxins: occurrence, toxicity and toxicokinetics. Toxins 9:228. https://doi.org/10.3390/toxins9070228 PubMed DOI PMC

Franceschi S, Bidoli E, Barón AE, La Vecchia C (1990) Maize and risk of cancers of the oral cavity, pharynx, and esophagus in northeastern Italy. J Natl Cancer Inst 82:1407–1411. https://doi.org/10.1093/jnci/82.17.1407 PubMed DOI

Frisvad JC, Samson RA (2004) Polyphasic taxonomy of Penicillium subgenus Penicillium-A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Stud Mycol 1–174

Frisvad JC, Smedsgaard J, Samson RA, Larsen TO, Thrane U (2007a) Fumonisin B2 production by Aspergillus niger. J Agric Food Chem 55:9727–9732. https://doi.org/10.1021/jf0718906 PubMed DOI

Frisvad JC, Thrane U, Samson RA (2007b) Mycotoxin producers. In: Dijksterhuis, J. & Samson, R.A. Food mycology: a multifaceted approach to fungi and food, volume 25. CRC Press, Boca Raton, US, pp 135–159

Gao X, Xiao Z, Li C, Zhang J, Zhu L, Sun L, Zhang N, Khalil MM, Rajput SA, Qi D (2018) Prenatal exposure to zearalenone disrupts reproductive potential and development via hormone-related genes in male rats. Food Chem Toxicol 116:11–19. https://doi.org/10.1016/j.fct.2018.04.011 PubMed DOI

Gareis M, Schothorst RC, Vidnes A, Bergsten C, Paulsen B, Brera C, Miraglia M (2003) SCOOP task 3.2.10. Collection of Occurrence Data of Fusarium Toxins in Food and Assessment of Dietary Intake by the Population of EU Member States

Girardet C, Bonnet MS, Jdir R, Sadoud M, Thirion S, Tardivel C, Roux J, Lebrun B, Wanaverbecq N, Mounien L, Trouslard J, Jean A, et al (2011a) The food-contaminant deoxynivalenol modifies eating by targeting anorexigenic neurocircuitry. PLoS One 6:e26134. https://doi.org/10.1371/journal.pone.0026134

Girardet C, Bonnet MS, Jdir R, Sadoud M, Thirion S, Tardivel C, Roux J, Lebrun B, Mounien L, Trouslard J, Jean A, Dallaporta M (2011b) Central inflammation and sickness-like behavior induced by the food contaminant deoxynivalenol: a PGE2-independent mechanism. Toxicol Sci 124:179–191. https://doi.org/10.1093/toxsci/kfr219 PubMed DOI

Gottschalk C, Barthel J, Engelhardt G, Bauer J, Meyer K (2009) Simultaneous determination of type A, B and D trichothecenes and their occurrence in cereals and cereal products. Food Addit Contam Part A 26:1273–1289. https://doi.org/10.1080/02652030903013260 DOI

Grenier B, Oswald I (2011) Mycotoxin co-contamination of food and feed: meta-analysis of publications describing toxicological interactions. World Mycotoxin J 4:285–313. https://doi.org/10.3920/WMJ2011.1281 DOI

Grusie T, Cowan V, Singh J, McKinnon J, Blakley B (2018) Proportions of predominant ergot alkaloids (Claviceps purpurea) detected in Western Canadian grains from 2014 to 2016. World Mycotoxin J 11:259–264. https://doi.org/10.3920/WMJ2017.2241 DOI

Haarmann T, Rolke Y, Giesbert S, Tudzynski P (2009) Ergot: from witchcraft to biotechnology. Mol Plant Pathol 10:563–577. https://doi.org/10.1111/j.1364-3703.2009.00548.x PubMed DOI PMC

Haque MA, Wang Y, Shen Z, Li X, Saleemi MK, He C (2020) Mycotoxin contamination and control strategy in human, domestic animal and poultry: a review. Microb Pathog 142:104095. https://doi.org/10.1016/j.micpath.2020.104095

Herrera M, Bervis N, Carramiñana JJ, Juan T, Herrera A, Ariño A, Lorán S (2019) Occurrence and exposure assessment of aflatoxins and deoxynivalenol in cereal-based baby foods for infants. Toxins 11:150. https://doi.org/10.3390/toxins11030150 PubMed DOI PMC

Heussner AH, Bingle LEH (2015) Comparative ochratoxin toxicity: a review of the available data. Toxins 7:4253–4282. https://doi.org/10.3390/toxins7104253 PubMed DOI PMC

IARC - International Agency for Research on Cancer (2012) Chemical agents and related occupations: a review of human carcinogens. IARC Press, Lyon, France. Available from: https://www.ncbi.nlm.nih.gov/books/NBK304416/

Iverson F, Armstrong C, Nera E, Truelove J, Fernie S, Scott P, Stapley R, Hayward S, Gunner S (1995) Chronic feeding study of deoxynivalenol in B6C3F1 male and female mice. Teratog Carcinog Mutagen 15:283–306. https://doi.org/10.1002/tcm.1770150606 PubMed DOI

JECFA FAO/WHO - Joint FAO/WHO Expert Committee on Food Additives (2018) Aflatoxins (addendum). In: JECFA WHO/FAO - Joint FAO/WHO Expert Committee on Food Additives. Safety evaluation of certain contaminants in food: Prepared by the eighty-third meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), WHO Food Additives Series No 74, pp 3–280. Available from: https://apps.who.int/iris/handle/10665/276868

JECFA FAO/WHO - Joint FAO/WHO Expert Committee on Food Additives (2011) Safety evaluation of certain contaminants in food: prepared by the Seventy-second meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), WHO Food Additives Series No 63, pp 1–799. Available from: https://apps.who.int/iris/handle/10665/44520

JECFA FAO/WHO - Joint FAO/WHO Expert Committee on Food Additives (2001) T-2 and HT-2 toxins. In: Joint FAO/WHO Expert Committee on Food Additives (Ed.), Safety Evaluation of Certain Food Additives and Contaminants, WHO Food Additives Series No 47. Available from: https://inchem.org/documents/jecfa/jecmono/v47je01.htm

JECFA FAO/WHO - Joint FAO/WHO Expert Committee on Food Additives (2000) Zearalenone. In: Joint FAO/WHO Expert Committee on Food Additives (Ed.), Safety Evaluation of Certain Food Additives and Contaminants, WHO Food Additives Series No 44. Available from: https://inchem.org/documents/jecfa/jecmono/v44jec01.htm

JECFA FAO/WHO - Joint FAO/WHO Expert Committee on Food Additives (2012) Fumonisins. In: Safety evaluation of certain food additives and contaminants : prepared by the Seventy fourth meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), WHO Food Additives Series No 65, pp 325–794

JECFA FAO/WHO - Joint FAO/WHO Expert Committee on Food Additives (1995) Evaluation of certain food additives and contaminants. Forty-fourth report of the Joint FAO/WHO Expert Committee on Food Additives., WHO Technical Report Series 859.

Karlovsky P, Suman M, Berthiller F, De Meester J, Eisenbrand G, Perrin I, Oswald IP, Speijers G, Chiodini A, Recker T, Dussort P (2016) Impact of food processing and detoxification treatments on mycotoxin contamination. Mycotox Res 32:179–205. https://doi.org/10.1007/s12550-016-0257-7 DOI

Klotz JL (2015) Activities and effects of ergot alkaloids on livestock physiology and production. Toxins (basel) 7:2801–2821. https://doi.org/10.3390/toxins7082801 PubMed DOI

Kuiper-Goodman T (1999) Approaches to the risk analysis of mycotoxins in the food supply. FNA/ANA 10–16

Kumar P, Mahato DK, Sharma B, Borah R, Haque S, Mahmut MMC, Shah AK, Rawal D, Bora H, Bui S (2020) Ochratoxins in food and feed: occurrence and its impact on human health and management strategies. Toxicon 187:151–162. https://doi.org/10.1016/j.toxicon.2020.08.031 PubMed DOI

Kunishige K, Kawate N, Inaba T, Tamada H (2017) Exposure to zearalenone during early pregnancy causes estrogenic multitoxic effects in mice. Reprod Sci 24:421–427. https://doi.org/10.1177/1933719116657194 PubMed DOI

Larsen J, Hunt J, Perrin I, Ruckenbauer P (2004) Workshop on trichothecenes with a focus on DON: summary report. Toxicol Lett 153:1–22. https://doi.org/10.1016/j.toxlet.2004.04.020 PubMed DOI

Larsen TO, Frisvad JC, Ravn G, Skaaning T (1998) Mycotoxin production by Penicillium expansum on blackcurrant and cherry juice. Food Addit Contam 15:671–675. https://doi.org/10.1080/02652039809374696 PubMed DOI

Lee C-H, Lee C-L, Pan T-M (2010) A 90-d toxicity study of monascus-fermented products including high citrinin level. J Food Sci 75:T91-97. https://doi.org/10.1111/j.1750-3841.2010.01626.x PubMed DOI

Lomax LG, Cole RJ, Dorner JW (1984) The toxicity of cyclopiazonic acid in weaned pigs. Vet Pathol 21:418–424. https://doi.org/10.1177/030098588402100408 PubMed DOI

Lorenz N, Dänicke S, Edler L, Gottschalk C, Lassek E, Marko D, Rychlik M, Mally A (2019) A critical evaluation of health risk assessment of modified mycotoxins with a special focus on zearalenone. Mycotoxin Res 35:27–46. https://doi.org/10.1007/s12550-018-0328-z PubMed DOI

Mahato DK, Lee KE, Kamle M (2019) Aflatoxins in food and feed: an overview on prevalence, detection and control strategies. Front Microbiol 10:1–10. https://doi.org/10.3389/fmicb.2019.02266 DOI

Malir F, Louda M, Toman J, Ostry V, Pickova D, Pacovsky J, Brodak M, Pfohl-Leszkowicz A (2021) Investigation of ochratoxin A biomarkers in biological materials obtained from patients suffering from renal cell carcinoma. Food Chem Toxicol 158:112669. https://doi.org/10.1016/j.fct.2021.112669

Malir F, Ostry V, Pfohl-Leszkowicz A, Malir J, Toman J (2016) Ochratoxin A: 50 years of research. Toxins 8:191. https://doi.org/10.3390/toxins8070191 PubMed DOI PMC

Marasas WF (2001) Discovery and occurrence of the fumonisins: a historical perspective. Environ Health Perspect 109:239–243 PubMed PMC

Missmer SA, Suarez L, Felkner M, Wang E, Merrill AH Jr, Rothman KJ, Hendricks KA (2006) Exposure to fumonisins and the occurrence of neural tube defects along the Texas-Mexico border. Environ Health Perspect 114:237–241. https://doi.org/10.1289/ehp.8221 PubMed DOI

Nazhand A, Durazzo A, Lucarini M, Souto EB, Santini A (2020) Characteristics, occurrence, detection and detoxification of aflatoxins in foods and feeds. Foods 9:644. https://doi.org/10.3390/foods9050644 PubMed DOI PMC

Nejati P, Chaychi Nosrati A, Bayat M, Lakzaie Azar O (2014) An investigation on measurement means of citrinin toxin quantity by toxigenic Aspergillus species in biomass, using ELISA. Int J Adv Biol 2:2466–2471

Nuehring LP, Rowland GN, Harrison LR, Cole RJ, Dorner JW (1985) Cyclopiazonic acid mycotoxicosis in the dog. Am J Vet Res 46:1670–1676 PubMed

Ohtsubo K, Ryu J-C, Nakamura K, Izumiyama N, Tanaka T, Yamamura H, Kobayashi T, Ueno Y (1989) Chronic toxicity of nivalenol in female mice: a 2-year feeding study with Fusarium nivale Fn 2B-moulded rice. Food Chem Toxicol 27:591–598. https://doi.org/10.1016/0278-6915(89)90018-5 PubMed DOI

Ojuri OT, Ezekiel CN, Sulyok M, Ezeokoli OT, Oyedele OA, Ayeni KI, Eskola MK, Šarkanj B, Hajslova J, Adeleke RA, Nwangburuka CC, Elliott CT et al (2018) Assessing the mycotoxicological risk from consumption of complementary foods by infants and young children in Nigeria. Food Chem Toxicol 121:37–50. https://doi.org/10.1016/j.fct.2018.08.025 PubMed DOI

Orlando B, Maumené C, Piraux F (2017) Ergot and ergot alkaloids in French cereals: occurrence, pattern and agronomic practices for managing the risk. World Mycotoxin J 10:327–338. https://doi.org/10.3920/WMJ2017.2183 DOI

Ostry V (2008) Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs. World Mycotoxin J 1:175–188. https://doi.org/10.3920/WMJ2008.x013 DOI

Ostry V, Dofkova M, Blahova J, Malir F, Kavrik R, Rehurkova I, Ruprich J (2020) Dietary exposure assessment of sum deoxynivalenol forms, sum T-2/HT-2 toxins and zearalenone from cereal-based foods and beer. Food Chem Toxicol 139:1–10. https://doi.org/10.1016/j.fct.2020.111280 DOI

Ostry V, Malir F, Cumova M, Kyrova V, Toman J, Grosse Y, Pospichalova M, Ruprich J (2018a) Investigation of patulin and citrinin in grape must and wine from grapes naturally contaminated by strains of Penicillium expansum. Food Chem Toxicol 118:805–811. https://doi.org/10.1016/j.fct.2018.06.022 PubMed DOI

Ostry V, Malir F, Ruprich J (2013) Producers and important dietary sources of ochratoxin A and citrinin. Toxins 5:1574–1586. https://doi.org/10.3390/toxins5091574 PubMed DOI PMC

Ostry V, Malir F, Toman J, Grosse Y (2017) Mycotoxins as human carcinogens - the IARC monographs classification. Mycotoxin Res 33:65–73. https://doi.org/10.1007/s12550-016-0265-7 PubMed DOI

Ostry V, Toman J, Grosse Y, Malir F (2018b) Cyclopiazonic acid: 50th anniversary of its discovery. World Mycotoxin J 11:135–148. https://doi.org/10.3920/WMJ2017.2243 DOI

Pazoutova S (2001) The phylogeny and evolution of the genus Claviceps. Mycol Res 105:275–283. https://doi.org/10.1017/S0953756201003562 DOI

Pestka JJ, Smolinski AT (2005) Deoxynivalenol: toxicology and potential effects on humans. J Toxicol Environ Health Part B 8:39–69. https://doi.org/10.1080/10937400590889458 DOI

Pickova D, Ostry V, Malir F (2021a) A recent overview of producers and important dietary sources of aflatoxins. Toxins 13:186. https://doi.org/10.3390/toxins13030186 PubMed DOI PMC

Pickova D, Ostry V, Toman J, Malir F (2021b) Aflatoxins: history, significant milestones, recent data on their toxicity and ways to mitigation. Toxins 13:399. https://doi.org/10.3390/toxins13060399 PubMed DOI PMC

Pinton P, Oswald IP (2014) Effect of deoxynivalenol and other type B trichothecenes on the intestine: a review. Toxins 6:1615–1643. https://doi.org/10.3390/toxins6051615 PubMed DOI PMC

Pitt JI (2000) Toxigenic fungi and mycotoxins. Br Med Bull 56:184–192. https://doi.org/10.1258/0007142001902888 PubMed DOI

Poppenberger B, Berthiller F, Lucyshyn D, Sieberer T, Schuhmacher R, Krska R, Kuchler K, Glössl J, Luschnig C, Adam G (2003) Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana. J Biol Chem 278:47905–47914. https://doi.org/10.1074/jbc.M307552200 PubMed DOI

Puel O, Galtier P, Oswald IP (2010) Biosynthesis and toxicological effects of patulin. Toxins (basel) 2:613–631. https://doi.org/10.3390/toxins2040613 PubMed DOI

Pustjens AM, Castenmiller JJM, te Biesebeek JD, de Rijk TC, van Dam RCJ, Boon PE (2022) Dietary exposure to mycotoxins of 1- and 2-year-old children from a Dutch Total Diet Study. World Mycotoxin J 15:85–97. https://doi.org/10.3920/WMJ2020.2676 DOI

Qi X, Yang X, Chen S, He X, Dweep H, Guo M, Cheng W-H, Xu W, Luo Y, Gretz N, Dai Q, Huang K (2014a) Ochratoxin A induced early hepatotoxicity: new mechanistic insights from microRNA, mRNA and proteomic profiling studies. Sci Rep 4:5163. https://doi.org/10.1038/srep05163 DOI

Qi X, Yu T, Zhu L, Gao J, He X, Huang K, Luo Y, Xu W (2014b) Ochratoxin A induces rat renal carcinogenicity with limited induction of oxidative stress responses. Toxicol Appl Pharmacol 280:543–549. https://doi.org/10.1016/j.taap.2014.08.030 PubMed DOI

Rached E, Hard GC, Blumbach K, Weber K, Draheim R, Lutz WK, Özden S, Steger U, Dekant W, Mally A (2007) Ochratoxin A: 13-week oral toxicity and cell proliferation in male F344/n rats. Toxicol Sci 97:288–298. https://doi.org/10.1093/toxsci/kfm042 PubMed DOI

Rafai P, Bata A, Ványi A, Papp Z, Brydl E, Jakab L, Tuboly S, Túry E (1995a) Effect of various levels of T-2 toxin on the clinical status, performance and metabolism of growing pigs. Vet Rec 136:485–489. https://doi.org/10.1136/vr.136.19.485 PubMed DOI

Rafai P, Tuboly S, Bata A, Tilly P, Ványi A, Papp Z, Jakab L, Túry E (1995b) Effect of various levels of T-2 toxin in the immune system of growing pigs. Vet Rec 136:511–514. https://doi.org/10.1136/vr.136.20.511 PubMed DOI

Ramos A, Sanchis V, Marín S (2011) The prehistory of mycotoxins: related cases from ancient times to the discovery of aflatoxins. World Mycotoxin J 4:101–112. https://doi.org/10.3920/WMJ2010.1268 DOI

Reddy P, Hemsworth J, Guthridge KM, Vinh A, Vassiliadis S, Ezernieks V, Spangenberg GC, Rochfort S (2020) Ergot alkaloid mycotoxins: physiological effects, metabolism and distribution of the residual toxin in mice. Sci Rep 10:1–10. https://doi.org/10.1038/s41598-020-66358-2 DOI

RIVM - Rijksinstituut voor Volksgezondheid en Milieu, National Institute for Public Health and the Environment (2017) Assessment of the toxicity of citrinin. National Institute for Public Health and the Environment. Ministry of Health, Welfare and Sport 1–20. Available from: https://www.rivm.nl/sites/default/files/2018-11/Asssessment%20of%20the%20toxicity%20of%20citrinin_def.pdf

Rossi F, Gallo A, Bertuzzi T (2020) Emerging mycotoxins in the food chain. Med J Nutrition Metab 13:7–27. https://doi.org/10.3233/MNM-190345 DOI

SCF - Scientific Committee on Food (2000) Minute Statement on Patulin Expressed by the Scientific Committee on Food during the plenary meeting on 8 March 2000. Available from: https://food.ec.europa.eu/system/files/2020-12/sci-com_scf_out55_en.pdf

SCHER/SCCP/SCENIHR - Scientific Committee on Health and Environmental Risks/Scientific Committee on Consumer Products/ Scientific Committee on Emerging and Newly Identified Health Risks (2009) Risk assessment methodologies and approaches for genotoxic and carcinogenic substances. Available from: https://ec.europa.eu/health/ph_risk/committees/04_scher/docs/scher_o_113.pdf

Schiefer HB, Nicholson S, Kasali OB, Hancock DS, Greenhalgh R (1985) Pathology of acute 3-acetyldeoxynivalenol toxicity in mice. Can J Comp Med 49:315–318 PubMed PMC

Schollenberger M, Drochner W, Müller H-M (2007) Fusarium toxins of the scirpentriol subgroup: a review. Mycopathologia 164:101–118. https://doi.org/10.1007/s11046-007-9036-5 PubMed DOI

Schulz MC, Schumann L, Rottkord U, Humpf HU, Gekle M, Schwerdt G (2018) Synergistic action of the nephrotoxic mycotoxins ochratoxin A and citrinin at nanomolar concentrations in human proximal tubule-derived cells. Toxicol Lett 291:149–157. https://doi.org/10.1016/j.toxlet.2018.04.014 PubMed DOI

Selvaraj JN, Wang Y, Zhou L, Zhao Y, Xing F, Dai X (2015) A review of recent mycotoxin survey data and advanced mycotoxin detection techniques reported from China. Food Addit Contam Part A 32:440–452. https://doi.org/10.1080/19440049.2015.1010185 DOI

Shephard GS (2008) Impact of mycotoxins on human health in developing countries. Food Addit Contam Part A 25:146–151. https://doi.org/10.1080/02652030701567442 DOI

Singh ND, Sharma AK, Patil RD, Rahman S, Leishangthem GD, Kumar M (2014) Effect of feeding graded doses of Citrinin on clinical and teratology in female Wistar rats. Indian J Exp Biol 52:159–167 PubMed

Sirot V, Fremy J-M, Leblanc J-C (2013) Dietary exposure to mycotoxins and health risk assessment in the second French total diet study. Food Chem Toxicol 52:1–11. https://doi.org/10.1016/j.fct.2012.10.036 PubMed DOI

Skarkova J, Ostry V, Malir F, Roubal T (2013) Determination of ochratoxin A in food by high performance liquid chromatography. Anal Lett 46:1495–1504 DOI

Smith M-C, Madec S, Coton E, Hymery N (2016) Natural co-occurrence of mycotoxins in foods and feeds and their in vitro combined toxicological effects. Toxins 8:1–36. https://doi.org/10.3390/toxins8040094 DOI

Snowdon AL (1990) Colour Atlas of postharvest diseases and disorders of fruits and vegetables. General Introduction and Fruits, 1st edn. Wolfe Scientific, Lonon

Speijers GJA (1993) Subchronic toxicity experiment with rats fed a diet containing ergotamine-tartrate. National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands

Speijers GJA, Speijers MHM (2004) Combined toxic effects of mycotoxins. Toxicol Lett 153:91–98. https://doi.org/10.1016/j.toxlet.2004.04.046 PubMed DOI

Steinkellner H, Binaglia M, Dall’Asta C, Gutleb AC, Metzler M, Oswald IP, Parent-Massin D, Alexander J (2019) Combined hazard assessment of mycotoxins and their modified forms applying relative potency factors: zearalenone and T2/HT2 toxin. Food Chem Toxicol 131:110599. https://doi.org/10.1016/j.fct.2019.110599

Stockmann-Juvala H, Savolainen K (2008) A review of the toxic effects and mechanisms of action of fumonisin B1. Hum Exp Toxicol 27:799–809. https://doi.org/10.1177/0960327108099525 PubMed DOI

Sugita-Konishi Y, Kubosaki A, Takahashi M, Park BJ, Tanaka T, Takatori K, Hirose M, Shibutani M (2008) Nivalenol and the targeting of the female reproductive system as well as haematopoietic and immune systems in rats after 90-day exposure through the diet. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:1118–1127. https://doi.org/10.1080/02652030802093892 PubMed DOI

Sugita-Konishi Y, Park BJ, Kobayashi-Hattori K, Tanaka T, Chonan T, Yoshikawa K, Kumagai S (2006) Effect of cooking process on the deoxynivalenol content and its subsequent cytotoxicity in wheat products. Biosci Biotechnol Biochem 70:1764–1768. https://doi.org/10.1271/bbb.50571 PubMed DOI

Sumarah MW (2022) The deoxynivalenol challenge. J Agric Food Chem 70:9619–9624. https://doi.org/10.1021/acs.jafc.2c03690 PubMed DOI

Takahashi M, Shibutani M, Sugita-Konishi Y, Aihara M, Inoue K, Woo G-H, Fujimoto H, Hirose M (2008) A 90-day subchronic toxicity study of nivalenol, a trichothecene mycotoxin, in F344 rats. Food Chem Toxicol 46:125–135. https://doi.org/10.1016/j.fct.2007.07.005 PubMed DOI

Tomar RS, Blakley BR, DeCoteau WE (1987) Immunological responsiveness of mouse spleen cells after in vivo or in vitro exposure to 3-acetyldeoxynivalenol. Food Chem Toxicol 25:393–398. https://doi.org/10.1016/0278-6915(87)90175-x PubMed DOI

van der Fels-Klerx H, Stratakou I (2010) T-2 toxin and HT-2 toxin in grain and grain-based commodities in Europe: occurrence, factors affecting occurrence, co-occurrence and toxicological effects. World Mycotoxin J 3:349–367. https://doi.org/10.3920/WMJ2010.1237 DOI

Voss KA (1990) In vivo and in vitro toxicity of cyclopiazonic acid (CPA). In: Llewellyn GC, O’Rear CE (eds) Biodeterioration research, vol 3. Mycotoxins, biotoxins, wood decay, air quality, cultural properties, general biodeterioration, and degradation. Springer Science, New York, NY, USA, pp 67–84 DOI

WHO/IPCS - World Health Organization/International Programme on Chemical Safety (2009). Principles and methods for the risk assessment of chemicals in food Environmental Health Criteria No 240, pp. 1–34. Available from: https://www.who.int/publications/i/item/9789241572408

Wild CP, Gong YY (2010) Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis 31:71–82. https://doi.org/10.1093/carcin/bgp264 PubMed DOI

Wogan GN, Paglialunga S, Newberne PM (1974) Carcinogenic effects of low dietary levels of aflatoxin B1 in rats. Food Chem Toxicol 12:681–685. https://doi.org/10.1016/0015-6264(74)90239-9 DOI

Wouters FA, Speijers GJA (1996) 847. Patulin (WHO Food Additives Series 35). World health organization food additives series 35. In: INCHEM (Internationally Peer Reviewed Chemical Safety Information). https://inchem.org/documents/jecfa/jecmono/v35je16.htm . Accessed 27 May 2022

Wu W, He K, Zhou H-R, Bethiller F, Adam G, Sugita-Konishi Y, Watanabe M, Krantis A, Durst T, Zhang H, Pestka JJ (2014a) Effects of oral exposure to naturally-occurring and synthetic deoxynivalenol congeners on proinflammatory cytokine and chemokine mRNA expression in the mouse. Toxicol Appl Pharmacol 278:107–115. https://doi.org/10.1016/j.taap.2014.04.016 PubMed DOI PMC

Wu W, Zhou H-R, Bursian SJ, Pan X, Link JE, Berthiller F, Adam G, Krantis A, Durts T, Pestka JJ (2014b) Comparison of anorectic and emetic potencies of deoxynivalenol (vomitoxin) to the plant metabolite deoxynivalenol-3-glucoside and synthetic deoxynivalenol derivatives EN139528 and EN139544. Toxicol Sci 142:167–181. https://doi.org/10.1093/toxsci/kfu166 PubMed DOI PMC

Zinedine A, Soriano JM, Moltó JC, Mañes J (2007) Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: an oestrogenic mycotoxin. Food Chem Toxicol 45:1–18. https://doi.org/10.1016/j.fct.2006.07.03 PubMed DOI

Mycotoxins in brewing and malting: is every sample contaminated with mycotoxins?

Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS