Burns are a major global healthcare concern, often complicated by the presence of bacteria such as Pseudomonas aeruginosa in the wounds. Silver-based dressings are commonly used in the treatment of burns but can cause skin irritation and delay healing time. Medical-grade honey (MGH) provides an interesting alternative. This study investigated the antimicrobial effects and possible cytotoxicity of L-Mesitran Soft (MGH-gel) and its individual components, Medihoney (Manuka), Flammazine (silver sulphadiazine), and silver nitrate (AgNO3) in an ex vivo human burn wound model. Bacterial survival and wound healing parameters, including re-epithelialization and keratinocyte proliferation were assessed. L-Mesitran, Flammazine, and AgNO3 reduced P. aeruginosa numbers below detection levels. L-Mesitran Soft exhibited a significantly stronger antimicrobial effect compared to Medihoney. The individual components of L-Mesitran contributed significantly to its antibacterial efficacy, thus suggesting synergistic activities. Moreover, L-Mesitran, Flammazine, and AgNO3 slightly inhibited re-epithelialization while Medihoney treatment resulted in a complete lack of re-epithelialization and keratinocyte proliferation. Furthermore, clinical cases illustrated the effectiveness of MGH therapy in infected burns. Overall, L-Mesitran Soft had similar effects as silver-based products on bacterial load and epidermal regeneration, but outperformed Medihoney. Therefore, supplemented MGH could be used as an effective alternative to silver-based dressings for P. aeruginosa-infected burns.

Bambino Gesu' Children's Hospital Research Institute Division of Plastic and Maxillofacial Surgery Department of Surgery Sant' Onofrio Square 4 00165 Rome Italy

Department of Health Sciences Faculty of Medicine Masaryk University Brno Czech Republic; Department of Public Health Faculty of Medicine Masaryk University Brno Czech Republic; College of Polytechnics Jihlava Jihlava Czech Republic

Preclinical Research Association of Dutch Burn Centers P O Box 1015 1940 AE Beverwijk the Netherlands

Preclinical Research Association of Dutch Burn Centers P O Box 1015 1940 AE Beverwijk the Netherlands; Plastic Reconstructive and Hand Surgery AUMC Amsterdam the Netherlands

Triticum Exploitatie BV Sleperweg 44 6222 NK Maastricht the Netherlands

Triticum Exploitatie BV Sleperweg 44 6222 NK Maastricht the Netherlands; Department of Gynecology and Obstetrics Maastricht University Medical Center 6202 AZ Maastricht the Netherlands

Wound Clinic Linksfield Road 45 Dowerglen Johannesburg 1612 South Africa; Department of Health Sciences Faculty of Medicine Masaryk University Brno Czech Republic; Department of Public Health Faculty of Medicine Masaryk University Brno Czech Republic

Citace poskytuje Crossref.org

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