The removal of copper (Cu) in soils by green technology is less treated with urgency, as it is a plant micronutrient. We examined the efficiency of Cu shoot accumulation by herbaceous plants in Cu-contaminated and non-contaminated soils in Trhové Dusniky and Podles, respectively, in the Czech Republic. The total soil Cu content of 81 mg kg-1 in Trhové Dusniky indicated a slight contamination level compared to 50 mg kg-1, the permissible value by WHO, and < 35 in Podlesí, representing a clean environment. The Cu content was above the permissible value in plants (10 mg kg-1 by WHO) in herbaceous speciesat the control site without trees: Stachys palustris L. (10.8 mg kg-1), Cirsium arvense L. (11.3 mg kg-1), Achillea millefolium L. (12.1 mg kg-1), Anthemis arvense L. (13.2 mg kg-1), and Calamagrostis epigejos L. (13.7 mg kg-1). In addition, Hypericum maculatum Crantz (10.6 mg kg-1), Campanula patula L. (11.3 mg kg-1), C. arvense (15 mg kg-1), and the highest accumulation in shoot of Equisetum arvense L. (37.1 mg kg-1), all under the canopy of trees at the uncontaminated site, were above the WHO value. Leucanthemum Vulgare (Lam.) and Plantago lanceolata L. recorded 11.2 mg kg-1 and 11.5 mg kg-1, respectively, in the soil of the Cu-contaminated site. These herbaceous species can support the phyto-management of Cu-contaminated soils, especially E. arvense. Critical attention is well-required in the medicinal application of herbaceous plants in treating human ailments due to their Cu accumulation potentials above the threshold. Spontaneous surveys and analysis of Cu speciation in herbaceous species can reveal suitable plants to decontaminate soils and provide caution on consumable products, especially bioactive compounds.

Department of Agricultural Food Environmental and Animal Sciences University of Udine Via Delle Scienze 206 33100 Udine Italy

Department of Agroenvironmental Chemistry and Plant Nutrition Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Prague Kamýcká 129 165 00 Prague 6 Czech Republic

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Ambrosini VG, Rosa DJ, Bastos de Melo GW, Zalamena J, Cella C, Simão DG, Souza da Silva L, Pessoa Dos Santos H, Toselli M, Tiecher TL, Brunetto G (2018) High copper content in vineyard soils promotes modifications in photosynthetic parameters and morphological changes in the root system of ‘Red Niagara’ plantlets. Plant Physiol Biochem 128:89–98. https://doi.org/10.1016/j.plaphy.2018.05.011 DOI

Amin H, Arain BA, Jahangir TM, Abbasi AR, Mangi J, Muhammad AS, Amin F (2019) Copper (Cu) tolerance and accumulation potential in four native plant species: a comparative study for effective phytoextraction technique. Geol Ecol Lands 5:53–64. https://doi.org/10.1080/24749508.2019.1700671 DOI

Andrejić G, Kovačević M, Dželetović Ž, Aleksić U, Grdović I, Rakić T (2023) Potentially toxic element accumulation in two Equisetum species spontaneously grown in the flotation tailings. J Serb Chem Soc 00:28–28. https://doi.org/10.2298/JSC230113028A DOI

Ariyakanon N, and Winaipanich B (2006) Phytoremediation of copper contaminated soil by Brassica juncea (L.) and Bidens alba (L.) DC. var. radiata. J Sci Res Chula Univ 31:49–56

Asare MO, Száková J (2023) Are anthropogenic soils from dumpsites suitable for arable felds? Evaluation of soil fertility and transfer of potentially toxic elements to plants. Plant Soil 486:307–322. https://doi.org/10.1007/s11104-023-05870-6 DOI

Asare MO, Apoh W, Afriyie JO, Horák J, Šmejda L, Hejcman M (2020) Traces of German and British settlement in soils of the Volta Region of Ghana. Geoder Reg 21:e00270. https://doi.org/10.1016/j.geodrs.2020.e00224 DOI

Asare MO, Száková J, Tlustoš P (2022) The fate of secondary metabolites in plants growing on Cd-, As-, and Pb-contaminated soils— a comprehensive review. Environ Sci Pollut Res 30:11378–11398. https://doi.org/10.1007/s11356-022-24776-x DOI

Asare MO, Száková J, Tlustoš P (2023) Mechanisms of As, Cd, Pb, and Zn hyperaccumulation by plants and their effects on soil microbiome in the rhizosphere. Front Environ Sci 11:1157415. https://doi.org/10.3389/fenvs.2023.1157415 DOI

Asgarpanah J, Roohi E (2012) Phytochemistry and pharmacological properties of Equisetum arvense L. J Med Plants Res 6:3689–3693. https://doi.org/10.5897/JMPR12.234 DOI

Boruvka L, Vacha R (2006) Litavka river alluvium as a model area heavily polluted with potentially risk elements. NATO Sci Ser: IV 68:267–298 DOI

Čadková Z, Vořechovská L, Javorská D et al (2023) The oral bioavailability of soil-borne risk elements for small terrestrial mammals: Microtus arvalis (Pallas, 1778) and Apodemus sylvaticus L. and its implication in environmental studies. Environ Sci Pollut Res 30:62397–62409. https://doi.org/10.1007/s11356-023-26437-z DOI

Cannon HL, Shacklette HT, Bastron H (1968) Metal absorption by Equisetum (horsetail). Retrieved from, https://pubs.usgs.gov/bul/1278a/report.pdf . Accessed on 19/05/2023

Chamberlain LA, Aguayo T, Zerega NJC, Dybzinski R, Egerton-Warburton LM (2022) Rapid improvement in soil health following the conversion of abandoned farm fields to annual or perennial agroecosystems. Front Sust Food Syst 6:1010298. https://doi.org/10.3389/fsufs.2022.1010298 DOI

Chaplygin V, Mandzhieva S, Minkina T, Sushkova S, Barahov A, Nevidomskaya D, Kızılkaya R, Gülser C, Chernikova N, Mazarji M, Iljina L, Rajput V (2020) Accumulating capacity of herbaceous plants of the Asteraceae and Poaceae families under technogenic soil pollution with zinc and cadmium. Eurasian J Soil Sci 9:165–172

Chen Y, Zhao HX, Xie ZH, Huang HY, Zang SY, Lian B (2015) Heavy metal pollution characteristics in the Kaili coal mining region, Guizhou province, China. J Resid Sci Technol 12:S123–S131 DOI

Coffey T (1993) The history and folklore of North American wildflowers. Facts on File. Available via DIALOG. https://www.google.com/search?q=The+History+and+Folklore+of+North+American+Wild+Flowers&rlz=1C1GCEU_csCZ981CZ981&oq=The+History+and+Folklor . Accessed 3 January 2023

Cuske M, Gerstyzn L, Karzewacka A (2013) The influence of pH on solubility of copper in soils contaminated by copper industry in Legnica. Civil Environ Eng 11:31–39

Dane T, Naidu R, Ming H, Megharaj M (2012) Copper phytotoxicity in native and agronomical plant species. Ecotoxicol Environ Saf 85:23–29. https://doi.org/10.1016/j.ecoenv.2012.08.018 DOI

Dhadse S (2022) Vermiconversion of textile industrial sludge; waste management and nutrients recycling. In: Ahmad F, Sultan M (Eds) Agricultural Waste - New Insights. IntechOpen. https://doi.org/10.5772/intechopen.107260

Elleuch A, Chaâbene Z, Grubb DC, Drira N, Mejdoub H, Khemakhem B (2013) Morphological and biochemical behavior of fenugreek (Trigonella foenum-graecum) under copper stress. Ecotoxicol Environ Saf 98:46–53. https://doi.org/10.1016/j.ecoenv.2013.09.028 DOI

Eske J (2020) Copper toxicity: symptoms and treatment. Retrieved from https://www.medicalnewstoday.com/articles/copper-toxicity . Accessed on 15/5/2023

Ettler V, Mihaljevič M, Šebek O, Nechutný Z (2007) Antimony availability in highly polluted soils and sediments – a comparison of single extractions. Chemosphere 68:455–463 DOI

Francisco D, Ângela de Barro M, Menezes C (2009) Pretreatment procedures applied to samples to be analyzed by neutron activation analysis at CDTN/CNEN. International Nuclear Atlantic Conference – INAC. Available via DIALOG. https://inis.iaea.org/collection/NCLCollectionStore/_Public/41/100/41100126.pdf . Accessed 9 October 2022

Fu L, Chen C, Wang B, Zhou X, Li S, Guo P, Shen Z, Wang G, Chen Y (2015) Differences in copper absorption and accumulation between copper-exclusion and copper-enrichment plants: a comparison of structure and physiological responses. PLoS One 10:e0133424. https://doi.org/10.1371/journal.pone.0133424 DOI

Gan HY, Schöning I, Schall P, Ammer C, Schrump M (2020) Soil organic matter mineralization as driven by nutrient stoichiometry in soils under differently managed forest stands. Front For Glob Chang. https://doi.org/10.3389/ffgc.2020.00099 DOI

Ghazaryan K, Movsesyan H, Ghazaryan N, Watts BA (2019) Copper phytoremediation potential of wild plant species growing in the mine-polluted areas of Armenia. Environ Pollut 249:491–501. https://doi.org/10.1016/j.envpol.2019.03.070 DOI

Goswami S, Das S (2016) Copper phytoremediation potential of Calandula officinalis L. and the role of antioxidant enzymes in metal tolerance. Ecotoxicol Environ Saf 126:211–218. https://doi.org/10.1016/j.ecoenv.2015.12.030 DOI

Hassler M (2022) World Ferns. Synonymic checklist and distribution of ferns and lycophytes of the world. Available via DIALOG. www.worldplants.de/ferns/ . Accessed 11 November 2022

Hill GM, Shannon MC (2019) Copper and zinc nutritional issues for agricultural animal production. Biol Trace Elem Res 188:148–159. https://doi.org/10.1007/s12011-018-1578-5 DOI

Jacob JM, Karthik C, Saratale RG, Kumar SS, Prabakar D, Kadirvelu K (2018) Biological approaches to tackle heavy metal pollution: a survey of literature. J Environ Manag 217:56–70. https://doi.org/10.1016/j.jenvman.2018.03.077 DOI

Janssen BH (1996) Nitrogen mineralization in relation to C: N ratio and decomposability of organic materials. Plant Soil 181:39–45 DOI

Kalra YP, Maynard DG (1991) Methods manual for forest soil and plant analysis. Edmonton: Forestry Canada, Northwest region, Northern Forestry Center. Information Report NOR-X-319, p 126

Kumar V, Pandita S, Sidhu GPS, Sharma A, Khanna K, Kaur P, Bali AS, Setia R (2021) Copper bioavailability, uptake, toxicity and tolerance in plants: a comprehensive review. Chemosphere 262:127810. https://doi.org/10.1016/j.chemosphere.2020.127810 DOI

Łuczaj Ł, Svanberg I, Köhler P (2011) Marsh woundwort, Stachys palustris L. (Lamiaceae): an overlooked food plant. Gen Res Crop Evol 58:783–793. https://doi.org/10.1007/s10722-011-9710-9 DOI

Magnusson B, Örnemark U (2014) Eurachem guide: the fitness for purpose of analytical methods – a laboratory guide to method validation and related topics, 2nd edn. Retrieved from,  https://www.eurachem.org/images/stories/Guides/pdf/MV_guide_2nd_ed_EN.pdf.  Accessed 8 Oct 2022

European Medicines Agency (2016) European Medicine Annual Report 2016. Available via DIALOG: https://www.ema.europa.eu/en/documents/annual-report/2016-annual-report-european-medicines-agency_en.pdf . Accessed 8 October 2022

USEPA Method 3052 (1996) Microwave assisted acid digestion of siliceous and organically based matrices. Available via DIALOG. https://www.epa.gov/sites/production/files/2015-12/documents/3052.pdf . Accessed 7 October 2022

Mir AR, Picthtel J, Hayat S (2021) Copper: uptake, toxicity, and tolerance in plants and management of Cu-contaminated soil. Biometal 34:737–759 DOI

Nworie OE, Qin J, Lin C (2019) Trace element uptake by herbaceous plants from the soils at a multiple trace element-contaminated site. Toxics 7(1):3. https://doi.org/10.3390/toxics7010003 DOI

Pampolino MF, Laureles EV, Gines HC, Buresh RJ (2008) Soil carbon and nitrogen changes in long-term continuous lowland rice cropping. Soil Sci Soc Amer J 72:798–807 DOI

Public notice No. 153/2016 (2016) about the conditions for the protection of agricultural soil quality. Legal code of the Czech Republic, pp 2692–2699

Reeves R (2006) Hyperaccumulation of trace elements by plants. In: Morel JL, Echevarria G, Goncharova N (Eds.) Phytoremediation of metal-contaminated soils. NATO Science Series, 68. Springer , Dordrecht

Sharma N, Bakshi A, Sharma A, Kaur I, Nagpa AK (2021) Health risk associated with copper intake through vegetables in different countries. IOP Conference Ser Earth Environ Sci 8:012071. https://doi.org/10.1088/1755-1315/889/1/012071 DOI

Song S, Wang S, Shi M, Hu S, Xu D (2022a) Urban blue-green space landscape ecological health assessment based on the integration of pattern, process, function, and sustainability. Sci Reports 12:7707. https://doi.org/10.1038/s41598-022-11960-9 DOI

Song P, Xu D, Yue J, Ma Y, Dong S, Feng J (2022b) Recent advances in soil remediation technology for heavy metal contaminated sites: a critical review. Sci Total Environ 838:156417. https://doi.org/10.1016/j.scitotenv.2022.156417 DOI

Stutz S, Parker C (2016) Leucanthemum vulgare (oxeye daisy). Available via DIALOG https://www.cabidigitallibrary.org/doi/full/10.1079/cabicompendium.13357 . Accessed 27 November 2022

Suman J, Uhlik O, Jitka VJ, Macek T (2018) Phytoextraction of heavy metals: a promising tool for clean-up of polluted environment? Front Plant Sci 9:1476. https://doi.org/10.3389/fpls.2018.01476 DOI

Verdejo J, Ginocchio R, Sauvé S, Salgado E, Neaman A (2015) Thresholds of copper phytotoxicity in field-collected agricultural soils exposed to copper mining activities in Chile. Ecotoxicol Environ Saf 122:171–177. https://doi.org/10.1016/j.ecoenv.2015.07.026 DOI

Vondráčková S, Száková J, Drábek O, Tejnecký V, Hejcman M, Müllerová V, Tlustoš P (2015) Aluminium uptake and translocation in Al hyperaccumulator Rumex obtusifolius is affected by low-molecular-weight organic acids content and soil pH. PLoS ONE 10:e0123351. https://doi.org/10.1371/journal.pone.0123351 DOI

Widmer J, Norgrove L (2023) Identifying candidates for the phytoremediation of copper in viticultural soils: a systematic review. Environ Res 216:114518 DOI

World Health Organization [WHO] (1996) Permissible limits of heavy metals in soil and plants. Switzerland, Geneva

World Health Organization [WHO] (2019) Lead poisoning and health, World Health Organization. Available Via DIALOG. https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health . Accessed 30 December 2022

Xu L, Xing X, Liang J, Penge J, Zhou J (2019) In situ phytoremediation of copper and cadmium in a co-contaminated soil and its biological and physical effects. RSC Adv 9:993–1003 DOI

Yang B, Tong X, Lan Z, Cui Y, Xie X (2018) Influence of the interaction between sphalerite and pyrite on the copper activation of sphalerite. Minerals 8:16. https://doi.org/10.3390/min8010016 DOI

Yruela I (2009) Copper in plants: acquisition, transport, and interactions. Funct Plant Biol 36:409–430 DOI

Zandi P, Yang J, Możdżeń K, Barabasz-Krasny B (2020) A review of copper speciation and transformation in plant and soil/wetland systems. Adv Agron 160:249–293. https://doi.org/10.1016/bs.agron.2019.11.001 DOI

Zárubová P, Hejcman M, Vondráčková S, Minka L, Száková J, Tlustoš P (2015) Distribution of P, K, Ca, Mg, Cd, Cu, Fe, Mn, Pb, and Zn in wood and bark age classes of willows and poplars used for phytoextraction on soils contaminated by risk elements. Environ Sci Pollu Res 22:18801–18813. https://doi.org/10.1007/s11356-015-5043-0 DOI

Zheng J, Wang H, Li Z, Tang S, Chen Z (2008) Using elevated carbon dioxide to enhance copper accumulation in Pteridium revolutum, a copper-tolerant plant, under experimental conditions. Int J Phytoremed 10:1161–1172. https://doi.org/10.1080/15226510801913934 DOI

Кabata-Pendias A (2011) Trace elements in soils and plants. CRC Taylor Fr Group, London New York

Abilities of herbaceous plant species to phytoextract Cd, Pb, and Zn from arable soils after poly-metallic mining and smelting