Mitigation of arsenic poisoning induced oxidative stress and genotoxicity by Ocimum gratissimum L
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
38184283
DOI
10.1016/j.toxicon.2024.107603
PII: S0041-0101(24)00009-6
Knihovny.cz E-resources
- Keywords
- Arsenic, Comet assay, Lymphocytes, Oryctolagus cuniculus L., Oxidative stress, Phytochemicals,
- MeSH
- Antioxidants pharmacology MeSH
- Arsenic * toxicity MeSH
- Ocimum * metabolism MeSH
- DNA metabolism MeSH
- Glutathione metabolism MeSH
- Rabbits MeSH
- Arsenic Poisoning * MeSH
- Oxidative Stress MeSH
- Hydrogen Peroxide MeSH
- DNA Damage MeSH
- Superoxide Dismutase metabolism MeSH
- Random Amplified Polymorphic DNA Technique MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antioxidants MeSH
- Arsenic * MeSH
- DNA MeSH
- Glutathione MeSH
- Hydrogen Peroxide MeSH
- Superoxide Dismutase MeSH
Arsenic toxicity is a major problem across the world due to geogenic activity and has been supposed to generate free radicals and genotoxicity among the arsenic-poisoned population. There is a need to find suitable free radical quenching compounds for the arsenic-induced free radical-affected population. In the present study, Na3AsO3- induced oxidative stress and genotoxicity were evaluated in Oryctolagus cuniculus L, and quenching competency of Ocimum species was examined by applying enzymatic and non-enzymatic in vitro tests, comet assay, and Random Amplified Polymorphic Deoxyribonucleic acid - Polymerase Chain Reaction (RAPD-PCR) methods. In the present study, oxidative damage due to Na3AsO3 intoxication in O. cuniculus L has been confirmed followed by substantive genotoxicity, and in a further study, it has also been reported that the extract of O. gratissimum L lowers the oxidative stress in experimental animals confirmed by a decrease in Malondialdehyde (MDA) 4.78 ± 0.05 (nmol/mg protein), and an increase in Glutathione (GSH) 2.87 ± 0.50 (μmoles/mg proteins), Superoxide Dismutase (SOD) 1.78 ± 0.03(Units/mg protein), Catalase (CAT) 2.72 ± 0.02 (μmoles of H2O2 consumed/min/mg proteins) and Glutathione peroxidase (GPX) 7.43 ± 0.01 (μg of glutathione utilized/min/mg protein). A positive impact of extract of O. gratissimum L on protection of genotoxicity has been also confirmed by Random Amplified Polymorphic DNA (RAPD) based reduction in polymorphic bands of Deoxyribonucleic acid (DNA) from 6.5 to 3.16 and comet assay-based increase in head DNA % (87.86 ± 1.58), tail moment (1.07 ± 0.27) and decrease in tail DNA % (12.13 ± 1.58) & tail length (8.2 ± 1.46) at 5% P in lymphocytes. A significant level reduction in free radicals and reduction in DNA polymorphism has proved the competency of test material for the development of suitable antidotes against arsenicosis.
Department of Biotechnology Government 5 Y T PG Autonomous College Durg Chhattisgarh India
Women Scientist Department of Science and Technology Government of India India
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