Biological activity of silver nanoparticles synthesized using viticultural waste
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
38484919
DOI
10.1016/j.micpath.2024.106613
PII: S0882-4010(24)00080-9
Knihovny.cz E-resources
- Keywords
- Antibiofilm, Antimicrobial, Cytotoxicity, Insect model, Silver nanoparticle, Vitis vinifera,
- MeSH
- Anti-Infective Agents pharmacology chemistry MeSH
- Biofilms drug effects MeSH
- Bombyx MeSH
- Cell Line MeSH
- Gram-Negative Bacteria drug effects MeSH
- Keratinocytes drug effects MeSH
- Metal Nanoparticles * chemistry MeSH
- Yeasts drug effects MeSH
- Larva drug effects MeSH
- Humans MeSH
- Microbial Sensitivity Tests * MeSH
- Plant Extracts pharmacology chemistry MeSH
- Silver * pharmacology chemistry metabolism MeSH
- Green Chemistry Technology MeSH
- Particle Size MeSH
- Cell Survival drug effects MeSH
- Vitis * chemistry MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Infective Agents MeSH
- Plant Extracts MeSH
- Silver * MeSH
This research paper presents a novel approach to the green synthesis of silver nanoparticles (AgNPs) using viticultural waste, allowing to obtain NP dispersions with distinct properties and morphologies (monodisperse and polydisperse AgNPs, referred to as mAgNPs and pAgNPs) and to compare their biological activities. Our synthesis method utilized the ethanolic extract of Vitis vinifera pruning residues, resulting in the production of mAgNPs and pAgNPs with average sizes of 12 ± 5 nm and 19 ± 14 nm, respectively. Both these AgNPs preparations demonstrated an exceptional stability in terms of size distribution, which was maintained for one year. Antimicrobial testing revealed that both types of AgNPs inhibited either the growth of planktonic cells or the metabolic activity of biofilm sessile cells in Gram-negative bacteria and yeasts. No comparable activity was found towards Gram-positives. Overall, pAgNPs exhibited a higher antimicrobial efficacy compared to their monodisperse counterparts, suggesting that their size and shape may provide a broader spectrum of interactions with target cells. Both AgNP preparations showed no cytotoxicity towards a human keratinocyte cell line. Furthermore, in vivo tests using a silkworm animal model indicated the biocompatibility of the phytosynthesized AgNPs, as they had no adverse effects on insect larvae viability. These findings emphasize the potential of targeted AgNPs synthesized from viticultural waste as environmentally friendly antimicrobial agents with minimal impact on higher organisms.
Department of Biotechnology and Life Sciences University of Insubria Varese Italy
Department of Biotechnology University of Chemistry and Technology Prague Prague Czech Republic
References provided by Crossref.org
Preparation and Biological Activity of Lignin-Silver Hybrid Nanoparticles
Synthesis and Characterization of Lignin-Silver Nanoparticles
