A novel negative-sense single-stranded RNA virus showing genetic similarity to viruses of the genus Rubodvirus has been found in raspberry plants in the Czech Republic and has tentatively been named raspberry rubodvirus 1 (RaRV1). Phylogenetic analysis confirmed its clustering within the group, albeit distantly related to other members. A screening of 679 plant and 168 arthropod samples from the Czech Republic and Norway revealed RaRV1 in 10 raspberry shrubs, one batch of Aphis idaei, and one individual of Orius minutus. Furthermore, a distinct isolate of this virus was found, sharing 95% amino acid identity in both the full nucleoprotein and partial sequence of the RNA-dependent RNA polymerase gene sequences, meeting the species demarcation criteria. This discovery marks the first reported instance of a rubodvirus infecting raspberry plants. Although transmission experiments under experimental conditions were unsuccessful, positive detection of the virus in some insects suggests their potential role as vectors for the virus.

Faculty of Science University of South Bohemia 370 05 Ceske Budejovice Czech Republic

Institute of Entomology Biology Centre Czech Academy of Sciences 370 05 Ceske Budejovice Czech Republic

Institute of Plant Molecular Biology Biology Centre Czech Academy of Sciences 370 05 Ceske Budejovice Czech Republic

Norwegian Institute of Bioeconomy Research 1433 Aas Norway

Research and Breeding Institute of Pomology Holovousy Ltd 508 01 Horice Czech Republic

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