Evaluation of Gelatin-Based Poly(Ester Urethane Urea) Electrospun Fibers Using Human Mesenchymal and Neural Stem Cells
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
2022.07258.PTDC
Fundação para a Ciência e a Tecnologia
LA/P/0037/2020
Associate Laboratory Institute of Nanostructures, Nanomodelling and Nanofabrication-i3N
UIDP/50025/2020
Associate Laboratory Institute of Nanostructures, Nanomodelling and Nanofabrication-i3N
PTDC/CTM-COM/32606/2017
Fundação para a Ciência e Tecnologia
UIDB/50025/2020
Fundação para a Ciência e Tecnologia
PubMed
39072995
DOI
10.1002/mabi.202400014
Knihovny.cz E-zdroje
- Klíčová slova
- electrospinning, gelatin, mesenchymal stem cells, neural stem cells, poly(ester urethane urea),
- MeSH
- biokompatibilní materiály chemie farmakologie MeSH
- buněčná adheze účinky léků MeSH
- buněčná diferenciace účinky léků MeSH
- kultivované buňky MeSH
- lidé MeSH
- mezenchymální kmenové buňky * cytologie účinky léků metabolismus MeSH
- nervové kmenové buňky * cytologie účinky léků metabolismus MeSH
- pevnost v tahu MeSH
- polyestery * chemie farmakologie MeSH
- polyurethany * chemie farmakologie MeSH
- proliferace buněk účinky léků MeSH
- testování materiálů MeSH
- tkáňové inženýrství * metody MeSH
- tkáňové podpůrné struktury chemie MeSH
- viabilita buněk účinky léků MeSH
- želatina * chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biokompatibilní materiály MeSH
- poly(ester urethane)urea MeSH Prohlížeč
- polyestery * MeSH
- polyurethany * MeSH
- želatina * MeSH
Previously, a new biodegradable poly(ester urethane urea) was synthesized based on polycaprolactone-diol and fish gelatin (PU-Gel). In this work, the potential of this new material for neural tissue engineering is evaluated. Membranes with randomly oriented fibers and with aligned fibers are produced using electrospinning and characterized regarding their mechanical behavior under both dry and wet conditions. Wet samples exhibit a lower Young's modulus than dry ones and aligned membranes are stiffer and more brittle than those randomly oriented. Cyclic tensile tests are conducted and high values for recovery ratio and resilience are obtained. Both membranes exhibited a hydrophobic surface, measured by the water contact angle (WCA). Human mesenchymal stem cells from umbilical cord tissue (UC-MSCs) and human neural stem cells (NSCs) are seeded on both types of membranes, which support their adhesion and proliferation. Cells stained for the cytoskeleton and nucleus in membranes with aligned fibers display an elongated morphology following the alignment direction. As the culture time increased, higher cell viability is obtained on randomfibers for UC-MSCs while no differences are observed for NSCs. The membranes support neuronal differentiation of NSCs, as evidenced by markers for a neuronal filament protein (NF70) and for a microtubule-associated protein (MAP2).
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague 4 Czech Republic
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