To date, evaluating the diets of natural enemies like carabids has largely been limited to spatially explicit and short-term sampling. This leaves a knowledge gap for the intra-annual dynamics of carabid diets, and the provision and timing of delivery of natural pest control services. Season-long pitfall trapping of adult carabids was conducted in conventional winter wheat fields, from November 2018 to June 2019, in five French departments. Diagnostic Multiplex PCR of carabid gut contents was used to determine the dynamics of carabid diets. The overall detection rate of target prey DNA was high across carabid individuals (80%) but varied with the prey group. The rate of detection was low for pests, at 8.1% for slugs and 9.6% for aphids. Detection of intraguild predation and predation on decomposers was higher, at 23.8% for spiders, 37.9% for earthworms and 64.6% for springtails. Prey switching was high at the carabid community level, with pest consumption and intraguild predation increasing through the cropping season as the availability of these prey increased in the environment, while the detection of decomposer DNA decreased. Variation in diet through the cropping season was characterized by: (i) complementary predation on slug and aphid pests; and (ii) temporal complementarity in the predominant carabid taxa feeding on each pest. We hypothesize that natural pest control services delivered by carabids are determined by complementary contributions to predation by the different carabid taxa over the season.

Agroécologie AgroSup Dijon INRAE Université de Bourgogne Franche Comté 17 rue Sully BP 86510 21065 Dijon CEDEX France

Functional Diversity in Agro Ecosystems Crop Research Institute Drnovská 507 Ruzyně 161 06 Praha 6 Czech Republic

IGEPP INRAE Institut Agro Université de Rennes 35000 Rennes France

IGEPP INRAE Institut Agro Université de Rennes 35653 Le Rheu France

Mountain Agriculture Research Unit Institute of Ecology University of Innsbruck Technikerstraße 25 6020 Innsbruck Austria

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