Scedosporium apiospermum and Lomentospora prolificans secrete siderophores (iron scavengers) during hyphal proliferation. Siderophores are virulence factors and potential clinical biomarkers of invasive scedosporiosis and lomentosporiosis. Both strains secreted a uniform spectrum of siderophores, including coprogen B (CopB), N α-methyl-coprogen B, dimethyl-coprogen, and ferricrocin, with N α-methyl-coprogen B being the fastest secreted and most abundant coprogen. Under iron and zinc restriction, reflecting a nutrient-limited host environment, L. prolificans secreted 45 times more CopB than did S. apiospermum, presumably contributing to its higher virulence. This robust mobilization of CopB was further enhanced by zinc surplus. Additionally, two novel cyclic peptides, Scedocyclin A and B, were characterized inScedosporium boydii using the de novo sequencing tool CycloBranch. Utilizing matrix-assisted laser desorption/ionization, the portfolio of coprogens detected had limits of detection and quantitation of 4.9 and 14.6 fmol/spot in complex matrices, respectively, making them strong candidates for the next-generation, routine diagnosis of invasive scedosporiosis and lomentosporiosis through the Biotyper siderotyping.

Department of Analytical Chemistry Faculty of Science Palacký University in Olomouc 17 listopadu 1192 12 779 00 Olomouc Czechia

Department of Bacteriology and Mycology National Reference Laboratory for Mycological Diagnostics Public Health Institute in Ostrava Partyzánské náměstí 2633 7 702 00 Ostrava Czechia

Department of Software Engineering Faculty of Information Technology Czech Technical University Prague Thákurova 9 160 00 Prague Czechia

Department of the Medical Microbiology second Faculty of Medicine Charles University and Motol University Hospital 5 Úvalu 84 150 06 Prague Czechia

Division of Infectious Diseases and Geographic Medicine Stanford University School of Medicine Foundation for Research in Infectious Diseases P O Box 2734 Saratoga California 95070 United States

Division of Parasitology and Mycology European Excellence Center in Medical Mycology National Reference Center on Chronic Aspergillosis Rennes University Hospital Inserm UMR_S 1085 Irset 2 Rue Henri le Guilloux 35033 Rennes France

Institute of Laboratory Medicine Faculty of Medicine University of Ostrava Syllabova 19 703 00 Ostrava Czechia

Laboratory of Molecular Structure Characterization Institute of Microbiology of the Czech Academy of Sciences Vídeňská 1083 142 00 Prague Czechia

Zobrazit více v PubMed

WHO fungal priority pathogens list to guide research, development and public health action. https://www.who.int/publications/i/item/9789240060241 (accessed Dec 9 , 2023).

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