Antibiotic resistance is increasing among Staphylococcus saprophyticus strains isolated from urinary tract infection. This necessitates alternative therapies. For this, a lytic phage (vB_SsapS-104) against S. saprophyticus, which formed round and clear plaques on bacterial culture plates, was isolated from hospital wastewater and characterized. Microscopy analysis showed that it had a small head (about 50 nm), tail (about 80 nm), and a collar (about 22 nm in length and 19 nm in width) indicating to be a phage within Siphoviridae family. Phage vB_SsapS-104 showed a large latency period of about 40 min, rapid adsorption rate that was significantly enhanced by MgCl2 and CaCl2, and high stability to a wide range of temperatures and pH values. Restriction analyses demonstrated that phage consists of a double-stranded DNA with an approximate genome size of 40 Kb. BLAST results did not show high similarity (megablast) with other previously identified phages. But, in Blastn, similarity with Staphylococcus phages was observed. Phage vB_SsapS-104 represented high anti-bacterial activity against S. saprophyticus isolates in vitro as it was able to lyse 8 of the 9 clinical isolates (%88.8) obtained from a hospital in Gorgan, Iran. It was a S. saprophyticus-specific phage because no lytic activity was observed on some other pathogenic bacteria tested. Therefore, phage vB_SsapS-104 can be considered as a specific virulent phage against of S. saprophyitcus isolated from urinary tract infection. This study provided the partial genomic characterization of S. saprophyticus phage and its application against urinary tract infection associated with S. saprophyticus. This phage also can be considered as a good candidate for a therapeutic alternative in the future.

• MeSH
• antibakteriální látky farmakologie   MeSH
• DNA virů MeSH
• fágová terapie MeSH
• genom virový MeSH
• hostitelská specificita MeSH
• infekce močového ústrojí mikrobiologie   MeSH
• koncentrace vodíkových iontů MeSH
• latence viru MeSH
• lidé MeSH
• odpadní voda virologie   MeSH
• sekvenční analýza DNA MeSH
• Siphoviridae genetika   izolace a purifikace   ultrastruktura   MeSH
• stafylokokové bakteriofágy genetika   MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus saprophyticus účinky léků   virologie   MeSH
• teplota MeSH
• transmisní elektronová mikroskopie MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Írán MeSH