UBL5 is an atypical ubiquitin-like protein, whose function in metazoans remains largely unexplored. We show that UBL5 is required for sister chromatid cohesion maintenance in human cells. UBL5 primarily associates with spliceosomal proteins, and UBL5 depletion decreases pre-mRNA splicing efficiency, leading to globally enhanced intron retention. Defective sister chromatid cohesion is a general consequence of dysfunctional pre-mRNA splicing, resulting from the selective downregulation of the cohesion protection factor Sororin. As the UBL5 yeast orthologue, Hub1, also promotes spliceosome functions, our results show that UBL5 plays an evolutionary conserved role in pre-mRNA splicing, the integrity of which is essential for the fidelity of chromosome segregation.

• MeSH
• adaptorové proteiny signální transdukční genetika   MeSH
• chromatidy genetika   MeSH
• HeLa buňky MeSH
• lidé MeSH
• ligasy genetika   MeSH
• mitóza genetika   MeSH
• oční proteiny genetika   metabolismus   MeSH
• prekurzory RNA genetika   MeSH
• proteiny buněčného cyklu genetika   MeSH
• regulace genové exprese MeSH
• Saccharomyces cerevisiae - proteiny genetika   MeSH
• segregace chromozomů genetika   MeSH
• sestřih RNA genetika   MeSH
• spliceozomy genetika   MeSH
• ubikvitiny genetika   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH